Junying Tang

Publications (4)4.45 Total impact

• Article: A peculiar site: melanoma of the cervix.

  Jing Zhang, Yi Cao, Lin Xiao, Junying Tang, Liangdan Tang
  American journal of obstetrics and gynecology 11/2011; 205(5):508.e1-3. · 3.28 Impact Factor
• Article: Caspase-3 Activation by Exponential Decay Nanosecond Pulsed Electric Fields on Tumor-Bearing BALB/c Nude Mice In Vivo

  Yan Mi, Chenguo Yao, Chengxiang Li, Caixin Sun, Junying Tang, Fangli Yang, Yanqing Wen
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  ABSTRACT: In order to study the apoptosis-induction effects of exponential decay nanosecond pulsed electric fields (EDnsPEF) in vivo , tumor models in 20 female BALB/c nude mice were established by inoculating them with human melanoma cells A375. These mice were randomly divided into treated group (exposed to EDnsPEF with intensity of 20 kV/cm and duration of 300 ns) and control group equally. Twenty days later, tumor growth in the treated group was effectively inhibited ( P <; 0.01 compared with that in control group), typical morphological apoptosis characteristics in ultrastructure were observed by transmission electron microscope, and expression of caspase-3 and caspase-3 messenger ribonucleic acid were obviously increased ( P <; 0.01) using immunofluorescence and RT-PCR, respectively. These experimental results contributed evidence of tumor-growth inhibition by EDnsPEF exposure in vivo . The mechanism was apoptosis-induction effects of EDnsPEF on cancer cells by activating caspase-3. This study presented in vivo evidence of caspase-3 activation for apoptosis-induction effects of EDnsPEF, and this supported possible drug-free tumor therapy utilizing EDnsPEF.
  IEEE Transactions on Plasma Science 09/2010; · 1.17 Impact Factor
• Conference Proceeding: Experiment and mechanism research of SKOV3 cancer cell apoptosis induced by nanosecond pulsed electric field

  Chenguo Yao,, Yan Mi,, Xiaoqian Hu,, Chengxiang Li,, Caixin Sun,, Junying Tang,, Xiaojuan Wu,
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  ABSTRACT: This paper studies the apoptosis of human ovarian carcinoma cell Line (SKOV3) induced by the nanosecond pulsed electric field (10kV/cm, 100ns, 1 Hz) and its effect on intracellular calcium concentration ([Ca²⁺] i ). These cells were doubly marked by Annexin V-FITC/PI, and the apoptosis rate was analyzed with flow cytometry. After AO/EB staining the morphological changes were observed under fluorescent microscope, and their ultrastructural changes were observed under scanning electron microscope (SEM). With Fluo-3/AM as calcium fluorescent marker, laser scanning confocal microscope (LSCM) was used to detect the effect of nsPEF on [Ca²⁺] i and the source of Ca²⁺. The results showed that the early apoptosis rate of the treatment group was (22.21±2.71)%, significantly higher than that of the control group (3.04±0.44)% (P<0.01). The typical features of apoptotic cell have been observed by fluorescent microscope and SEM. It is proved that nsPEF can induce apoptosis of SKOV3 cells and result in distinct increase in [Ca²⁺] i (P<0.01), which was independent of extracellular calcium concentration (P>0.05). Since nsPEF can penetrate cell membrane due to its high frequency components, one of the mechanisms of nsPEF-induced apoptosis may be that activating intracellular calcium stores can increase the [Ca²⁺] i , and consequently, the apoptotic signal pathway can be induced.
  Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE; 09/2008
• Article: Experiment and mechanism research of SKOV3 cancer cell apoptosis induced by nanosecond pulsed electric field.

  Chenguo Yao, Yan Mi, Xiaoqian Hu, Chengxiang Li, Caixin Sun, Junying Tang, Xiaojuan Wu
  [show abstract] [hide abstract]
  ABSTRACT: This paper studies the apoptosis of human ovarian carcinoma cell Line (SKOV3) induced by the nanosecond pulsed electric field (10kV/cm, 100ns, 1 Hz) and its effect on intracellular calcium concentration ([Ca2+]i). These cells were doubly marked by Annexin V-FITC/PI, and the apoptosis rate was analyzed with flow cytometry. After AO/EB staining the morphological changes were observed under fluorescent microscope, and their ultrastructural changes were observed under scanning electron microscope (SEM). With Fluo-3/AM as calcium fluorescent marker, laser scanning confocal microscope (LSCM) was used to detect the effect of nsPEF on [Ca2+]i and the source of Ca2+. The results showed that the early apoptosis rate of the treatment group was (22.21+/-2.71)%, significantly higher than that of the control group (3.04+/-0.44)% (P<0.01). The typical features of apoptotic cell have been observed by fluorescent microscope and SEM. It is proved that nsPEF can induce apoptosis of SKOV3 cells and result in distinct increase in [Ca2+]i (P0.01), which was independent of extracellular calcium concentration (P>0.05). Since nsPEF can penetrate cell membrane due to its high frequency components, one of the mechanisms of nsPEF-induced apoptosis may be that activating intracellular calcium stores can increase the [Ca2+]i, and consequently, the apoptotic signal pathway can be induced.
  Conference proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 02/2008; 2008:1044-7.