Robert Kubina

Publications of Robert Kubina

• [The influence of oxidised cholesterol derivatives on morphology of hepatocytes in rat's liver].

  Authors: Anna Mazur, Andrzej Wiczkowski, Robert Kubina

  Wiadomości lekarskie (Warsaw, Poland : 1960). 01/2011; 64(2):79-83.

  Products of oxidizing cholesterol-called oxysterols, are having different physicochemical properties and various from not-modified sterols, biological activity. Amongst characteristic effects ofProducts of oxidizing cholesterol-called oxysterols, are having different physicochemical properties and various from not-modified sterols, biological activity. Amongst characteristic effects of these connections are: stopping the activity of HMG-CoA reductase, antiproliferative action, apoptosis induction, changes in the structure and functioning of the cell membrane and influence on remodeling of the immunological system. Mechanisms of oxysterols influence on the cellular level include a broad spectrum of complicated biological activities and they haven't been examined to the end yet. In order to experiment execution the rat livers have been collected and the microscope sections have been made by standard technics, staining by haematoxylin and eosin (H&E). In each preparation 222 hepatic cells surrounding 10 central veins have been analysed and 222 hepatic cells occuring around 10 triads. The total surface ofcell, nucleus and cytoplasm have been taken for analysis. In all researched cases the analysed parameters have been reduced in area liver traid and central vein. Decreasing of area value of cell, nucleus and cytoplasm have been observed. On the basis of our examinations we determine, that oxysterols derived from the diet have influence on parenchymatous liver cells morphology in regions of hepatic triad and the central vein. We have concluded, that cytotoxic effect of oxysterols depend mainly on stopping the growth of these hepatic cells and lead to many disorders connected with the influence ofoxysterols, not only to cells but also to the all organism.

• [Concentration of soluble ligand for receptor CD30 (sCD30L)--marker of apoptosis in women with ovarian tumor]

  Authors: Aleksandra Mielczarek-Palacz, Zdzisława Kondera-Anasz, Justyna Sikora, Krzysztof Sodowski, Jerzy Switała, Robert Kubina

  Ginekologia polska. 07/2009; 80(7):494-7.

  OBJECTIVES: Impairments of apoptosis processes are the basis of pathogenesis of many diseases, including ovarian tumors. The aim of the study was to evaluated the concentration of soluble ligand forOBJECTIVES: Impairments of apoptosis processes are the basis of pathogenesis of many diseases, including ovarian tumors. The aim of the study was to evaluated the concentration of soluble ligand for receptor CD30 (sCD30L)--marker of apoptosis in women with ovarian tumor. MATERIAL AND METHODS: The study comprised 80 women, aged from 21 to 62, and included 30 patients with Cystadenocarcinoma serosum la, 35 with Cystadenoma serosum and 15 women with Teratoma maturum. The control group consisted of 30 healthy women, aged 24 to 57, with no evidence of pathological disorders in the reproductive system. The concentration of sCD30L in the serum of all studied women and in the fluid of ovarian cyst of women with cystadenoma serous were measured by immunoenzymatic method ELISA. RESULTS: The highest level of sCD30L was observed in the serum of women with ovarian cancer and it was significantly higher when compared to the concentration in the serum of women with cystadenoma serous and teratoma maturum of the ovary (p < 0.0001). In the fluid of ovarian cyst, the concentration of this marker was significantly higher in comparison with the level in the serum (p < 0.0001). CONCLUSIONS: In women with ovarian tumors we observed impairments of the apoptosis process, which is associated with an increased concentration of sCD30L in the serum. These changes are more intense in women with ovarian cancer. Higher level of the study parameter in the fluid of ovarian cyst is associated with the local immune response suppression.