Publications (2)7.68 Total impact
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Article: Effects of DAPT and Atoh1 overexpression on hair cell production and hair bundle orientation in cultured Organ of Corti from neonatal rats.
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ABSTRACT: In mammals, hair cells do not undergo spontaneous regeneration when they are damaged and result in permanent hearing loss. Previous studies in cultured Organ of Corti dissected from neonatal animals have shown that both DAPT (r-secretase inhibitor in the Notch signal pathway) treatment and Atoh1 overexpression can induce supernumerary hair cells. The effects of simultaneous DAPT treatment and Atoh1 over expression in the cells of cultured Organ of Corti from neonatal rats are still obscure. In this study, we set out to investigate the interaction of DAPT treatment and Atoh1 overexpression as well as culture time and the location of basilar fragment isolated form neonatal rat inner ear. Our results showed that DAPT treatment induced more hair cells in the apical turn, while Atoh1 overexpression induced more extra hair cells in the middle turn of the cultured Organ of Corti. When used together, their effects are additive but not synergistic. In addition, the induction of supernumerary hair cells by both DAPT and Atoh1 overexpression is dependent on the treatment time and the location of the cochlear tissue. Moreover, DAPT treatment causes dramatic changes in the orientation of the stereociliary bundles of hair cells, whereas Atoh1 overexpression didn't induce drastic change of the polarity of stereociliary bundles. Taken together, these results suggest that DAPT treatment are much more potent in inducing supernumerary hair cells than Atoh1 overexpression and that the new hair cells mainly come from the trans-differentiation of supporting cells around hair cells. The orientation change of stereociliary bundle of hair cells may be attributed to the insertion of the newly formed hair cells. The immature hair bundles on the newly formed hair cells may also contribute to the overall chaos of the stereociliary bundle of the sensory epithelia.PLoS ONE 01/2011; 6(10):e23729. · 4.09 Impact Factor -
Article: The modulation function and realizing method of holographic functional screen.
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ABSTRACT: The modulation function of holographic functional screen (HFS) in the real-time, large-size full-color (RLF), three-dimensional (3D) display system is derived from angular spectrum analysis. The directional laser speckle (DLS) method to realize the HFS is proposed. A HFS by the DLS method was fabricated and used in the experiment. Experimental results show that the HFS is valid in the RLF 3D display, and that the derived modulation function is valuable for the design of the HFS. The research results are important to realize the RLF 3D display system which will find many applications such as holographic video.Optics Express 12/2010; 18(26):27820-6. · 3.59 Impact Factor
