[Show abstract][Hide abstract] ABSTRACT: A novel Gram-stain positive, aerobic, non-motile, spore-forming actinobacterium, designated YIM 75926(T), was isolated from a soil sample collected at soil forest in Yuanmo county of Yunnan province, south-west China. Its taxonomic position was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain YIM 75926(T) belongs to the genus Pseudonocardia and was closely related to Pseudonocardia halophobica DSM 43089(T) (98.1% similarity). Strain YIM 75926(T) had MK-8 (H(4)) as the predominant menaquinone. The whole organism hydrolysates mainly consisted of meso-diaminopimelic acid, mannose, glucose, galactose and arabinose. The major cellular fatty acids were iso-C(16:0) (37.16%) and C(16:0) (12.43%). The DNA G+C content of strain YIM 75926(T) was 70.6 mol%. The resultant phylogenetic trees further showed that strain YIM 75926(T) belong to Pseudonocardia and had a distinct subclade within the evolutionary radiation of the genus Pseudonocardia. On the basis of its comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 75926(T) represent a novel species of the genus Pseudonocardia, named Pseudonocardia yuanmoensis sp. nov. The type strain is YIM 75926(T) (=CCTCC AA 2011017(T) = JCM 18055(T)).
Antonie van Leeuwenhoek 12/2011; 101(4):753-60. DOI:10.1007/s10482-011-9689-4 · 1.81 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A novel actinomycete strain, designated YIM 75904T, was isolated from a soil sample collected from dry-hot river valley in Dongchuan county, Yunnan province, south-west China. Its taxonomic status was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences clearly showed that the isolate YIM 75904T formed a distinct clade within the genus Amycolatopsis, and was closely related to Amycolatopsis sacchari K24T (99.3% similarity). Strain YIM75904T had a type-IV cell wall lacking of mycolic acids. Organism had MK-9(H4) as the predominant menaquonine, contained meso-diaminopimelic acid (meso-DAP), galactose, glucose, and arabinose as cell wall components, iso-C16:0, iso-C15:0, anteiso-C17:0 and anteiso-C15:0 as the major cellular fatty acids. The DNA G+C content of strain YIM 75904T was 68.5 mol%. The results of physiological and biochemical tests and DNA-DNA hybridization displayed the phenotypic and genotypic characters of strain YIM 75904T were different from its closest phylogenetic neighbour. Therefore, it is proposed that strain YIM 75904T represents a novel species of the genus Amycolatopsis, for which named Amycolatopsis dongchuanensis sp. nov.. The type strain is YIM 75904T (=CCTCC AA 2011016T =JCM 18054T).
International Journal of Systematic and Evolutionary Microbiology 12/2011; DOI:10.1099/ijs.0.038125-0 · 2.51 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A strain of thermotolerant actinomycete, designated YIM 75948(T), was isolated from a soil sample in Yunnan province, China. The strain grew at 25-55 °C (optimum 37 °C). The substrate mycelium and aerial mycelium produced on Czapek's agar were both pale yellow to white. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were mannose, ribose, glucose, galactose and arabinose. The major fatty acids were iso-C(15:0), iso-C(16:0) and C(16:0) and the predominant respiratory quinone was MK-9(H(4))(.) The polar lipids consisted of phosphatidylethanolamine, phosphatidylethanolamine with hydroxy fatty acids, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. The genomic DNA G+C content was 73.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 75948(T) belonged in the genus Actinokineospora and that its closest relative among recognized species was Actinokineospora fastidiosa DSM 43855(T) (97.6% sequence similarity). The mean level of DNA-DNA relatedness between the novel strain and A. fastidiosa DSM 43855(T) was, however, only 47.8%. Based on the phenotypic, chemotaxonomic and phylogenetic data and the results of the DNA-DNA hybridizations, strain YIM 75948(T) represents a novel species of the genus Actinokineospora for which the name Actinokineospora soli sp. nov. is proposed. The type strain is YIM 75948(T) ( =JCM 17695(T) =DSM 45613(T)). The description of the genus Actinokineospora is emended to reflect the fact that the genomic DNA G+C contents of A. fastidiosa DSM 43855(T) and the type strain of Actinokineospora soli sp. nov. recorded in the present study fell above the range given in previous descriptions of this genus.
International Journal of Systematic and Evolutionary Microbiology 10/2011; 62(Pt 8):1845-9. DOI:10.1099/ijs.0.035832-0 · 2.51 Impact Factor