Publications (2)1.84 Total impact
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Article: Y chromosome Microdeletions in Infertile Men with Severe Oligozoospermia
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ABSTRACT: This study was designed to determine the correlation between Y chromosome azoospermia factor (AZF) subregions microdeletions and severe oligozoospermia in Infertile Men. Subjects included 89 infertile men with severe oligozoospermia who had been referred to the Royan-2 infertility center for assisted reproduction. DNA was isolated from blood samples. Polymerase chain reaction (PCR) amplification of 6 loci spanning the AZFa, AZFb and AZFc subregions of the Y chromosome using sY84, sY86, sY127, sY134, sY254 and sY255 was performed. Microdeletions of the Y chromosome were found in 26 of the patients (29%). Microdeletions in the AZFa subregion (61%) were encountered more often than in AZFb (21%) and in AZFc (18%) in the patients. Our study showed that men with severe oligozoospermia should be evaluated for Yq11 microdeletions before deciding to operate varicoceles or else scheduling them for assisted reproductive techniques and there is a specific correlation between Y chromosome AZF subregions microdeletions and severe oligozoospermia.Journal of Basic and Applied Scientific Research. 02/2013; 3:786-791. -
Article: The prevalence of common CFTR mutations in Iranian infertile men with non-CAVD obstructive azoospermia by using ARMS PCR techniques.
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ABSTRACT: PURPOSE: To evaluate five common cystic fibrosis trans-membrane conductance regulator (CFTR) mutations (ΔF508, G542X, R117H, W1282X and N1303K) in the Iranian infertile men with noncongenital absence of vas deferens (CAVD) obstructive azoospermia. METHODS: The common CFTR gene mutations were tested on blood samples from 53 infertile men with non-CAVD obstructive azoospermia and 50 normal men as control individuals. Genomic DNA is extracted from the whole blood and the common CFTR mutations have been detected by the amplification refractory mutation system (ARMS) techniques. RESULTS: The common CFTR mutations were found positive in 5/53)9.43%(for ΔF508 and 4/53)7.55%(for G542X mutation of all patients tested. Also, no CFTR mutations were detected in the normal men. CONCLUSION: The common CFTR mutations were detected in 9/53(17%) infertile men with non-CAVD obstructive azoospermia. Pre-treatment CFTR mutation analysis remains critical to distinguish cystic fibrosis (CF) genotypes for men with non CAVD obstructive azoospermia.Journal of Assisted Reproduction and Genetics 10/2011; · 1.84 Impact Factor
