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Guo-Wei Li,
Xi-Ying Zhang,
Chun-Sheng Wang,
Yan-Jiao Zhang,
Xue-Wei Xu,
Chang Liu,
Hai Li,
Ang Liu,
Zhong Xu,
Xiao-Yan Song,
Xiu-Lan Chen,
Bai-Cheng Zhou,
Yu-Zhong Zhang
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ABSTRACT: A Gram-negative, aerobic, catalase- and oxidase- positive, non-flagellated, rod-shaped bacterium, designated strain P-50-3T, was isolated from seawater of the Pacific. The strain grew at 10-40oC (optimum at 30oC) and with 0-12% (w/v, optimum with 2%) NaCl. It reduced nitrate to nitrite but did not hydrolyze gelatin, starch or Tween 80. Analysis of 16S rRNA gene sequences showed that strain P-50-3T clustered tightly with the genus Albimonas and shared the highest 16S rRNA gene sequence similarity (94.3%) with type strain of Albimonas donghaensis. The major respiratory quinone was Q-10 and the major cellular fatty acids were C18:1ω7c, C18:0, 11-methyl C18:1ω7c and C16:0. Polar lipids included phosphatidylglycerol (PG), phosphatidylcholine (PC), two unidentified aminolipids and an unidentified lipid. The genomic G+C content of strain P-50-3T was 69.0 mol%. On the basis of the polyphasic data obtained in this study, strain P-50-3T represents a novel species within the genus Albimonas, for which the name Albimonas pacifica sp. nov. is proposed. The type strain of Albimonas pacifica is P-50-3T (=KACC 16527T=CGMCC 1.11030T). An emended description of the genus Albimonas Lim et al. 2008 is also proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2013; · 2.11 Impact Factor
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ABSTRACT: A yellow-colored, rod-shaped, Gram-negative and facultatively aerobic gliding bacterium, designed strain P7-3-5T, was isolated from intertidal sand of Yellow Sea, China. Analysis of 16S rRNA gene sequences revealed that strain P7-3-5T formed a distinct lineage within the family Flavobacteriaceae, sharing 94.2-96.9% sequence similarities with type strains of the most closely related genera including Hyunsoonleella, Jejuia, Marinivirga and Algibacter. The strain grew at 4-40°C and with 0.5-5.0% (w/v) NaCl. It reduced nitrate to nitrite and hydrolyzed gelatin and DNA. The major cellular fatty acids were iso-C15:0, iso-C15:1 G and anteiso-C15:0 and the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine (PE), three unidentified aminolipids (AL1-3) and four unidentified lipids (L1-4). Genomic DNA G+C content of strain P10-2-4T was 32.1%. The polyphasic data from this study suggested that strain P7-3-5T represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Arenitalea lutea gen. nov., sp. nov. is proposed. The type strain of Arenitalea lutea is P7-3-5T (=CGMCC 1.12213T= KACC 16457T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
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Xi-Ying Zhang, Guo-Wei Li,
Chun-Sheng Wang,
Yan-Jiao Zhang,
Xue-Wei Xu,
Hai Li,
Ang Liu,
Chang Liu,
Bin-Bin Xie,
Qi-Long Qin,
Zhong Xu,
Xiu-Lan Chen,
Bai-Cheng Zhou,
Yu-Zhong Zhang
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ABSTRACT: A marine prosthecate bacterium, designated strain P-1km-3T, was isolated from deep seawater of the Pacifica. Cells of strain P-1km-3T were Gram-negative, aerobic, catalase- and oxidase- positive, dimorphic rods with a single polar prostheca or flagellum. The strain hydrolyzed gelatin and grew at 4-40°C (optimum at 30°C) and with 0.5-12% (w/v, optimum with 2%) NaCl. Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain P-1km-3T belonged to the family Hyphomonadaceae in the class Alphaproteobacteria and represented a separate lineage, located between the genera Oceanicaulis and Woodsholea. Sequence similarities of strain P-1km-3T with type strains of Oceanicaulis and Woodsholea species were 93.2-93.9%. The predominant cellular fatty acids in strain P-1km-3T were C18:1ω7c, C18:0, 11-methyl C18:1ω7c, C17:0 and C19:0 cyclo ω8c. The major respiratory quinone of strain P-1km-3T was Q-10. Polar lipids of strain P-1km-3T comprised glucuronopyranosyldiglyceride (GUDG), mono-glycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG), an unidentified phospholipid (PL) and an unidentified lipid (L). The genomic G+C content of strain P-1km-3T was 66.0 mol%. On the basis of the polyphasic data presented in this study, strain P-1km-3T is proposed to represent a novel genus and species, namely Marinicauda pacifica gen. nov., sp. nov., within the family Hyphomonadaceae. The type strain of Marinicauda pacifica is P-1km-3T (=KACC 16526T=CGMCC 1.11031T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2012; · 2.11 Impact Factor
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Ang Liu,
Xi-Ying Zhang,
Chun-Xiao Chen,
Bin-Bin Xie,
Qi-Long Qin,
Chang Liu, Guo-Wei Li,
Hai Li,
Zhong Xu,
Xiu-Lan Chen,
Bai-Cheng Zhou,
Yu-Zhong Zhang
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ABSTRACT: A Gram-negative, motile, aerobic, oxidase and catalase-positive and rod-shaped bacterium, designed strain P10-2-4T, was isolated from an intertidal sand sample collected from coastal area of Qingdao (Yellow Sea), China. The strain reduced nitrate to nitrite and grew at 4-33°C and with 0.5-12% (w/v) NaCl. The predominant cellular fatty acids were summed feature 3 (C16:1ω7c and/or iso-C15:02-OH), C18:1ω7c and C16:0. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The major respiratory quinone was Q-8. Genomic DNA G+C content of strain P10-2-4T was 45.1%. Phylogenetic analyses of 16S rRNA gene sequences revealed that strain P10-2-4T belonged to the genus Neptunomonas, sharing the highest 16S rRNA gene sequence similarity (98.1%) with type strain of Neptunomonas japonica and 96.9%, 96.5% and 95.9% 16S rRNA gene sequence similarities with those of N. antarctica, N. concharum and N. naphthovorans, the other three recognized Neptunomonas species. But DNA-DNA relatedness value between strain P10-2-4T and its closest phylogenetic relative N. japonica JCM 14595T was 35.6%. Furthermore, strain P10-2-4T could be distinguished from representatives of known species in the genus Neptunomonas by combination of several phenotypic characteristics, such as salt and temperature tolerance, nitrate reduction, DNase activity and assimilation of substrates. Data from this polyphasic taxonomic study suggested that strain P10-2-4T represents a novel species in the genus Neptunomonas, for which the name Neptunomonas qingdaonensis sp. nov. is proposed. The type strain of Neptunomonas qingdaonensis is P10-2-4T (=CGMCC 1.10971T= KCTC 23686T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2012; · 2.11 Impact Factor
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Chun-Xiao Chen,
Xi-Ying Zhang,
Chang Liu,
Yong Yu,
Ang Liu, Guo-Wei Li,
Hai Li,
Xiu-Lan Chen,
Bo Chen,
Bai-Cheng Zhou,
Yu-Zhong Zhang
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ABSTRACT: A Gram-negative, aerobic, non-motile, pink-pigmented and rod-shaped strain, designated ZS3-33T, was isolated from Antarctic intertidal sandy sediment. The strain grew optimally at 15°C and with 1.0% (w/v) NaCl. It reduced nitrate to nitrite and hydrolyzed Tween 20. It could not produce Bacteriochlorophyll a. The predominant cellular fatty acid was C18:1ω7c and the predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, two unidentified aminophospholipids and an unidentified aminolipid. Analyses of 16S rRNA gene sequences revealed that strain ZS3-33T belonged to the genus Pseudorhodobacter, showing 97.4% 16S rRNA gene sequence similarity to type strain of Pseudorhodobacter ferrugineus and 95.3% sequence similarity to type strain of P. aquimaris. GyrB gene sequence similarities of strain ZS3-33T to type strains of P. ferrugineus and P. aquimaris were 87.6% and 81.7%, respectively. DNA-DNA relatedness between strain ZS3-33T and P. ferrugineus DSM 5888T was 56.6%. Genomic DNA G+C content of strain ZS3-33T was 57.1 mol%. Based on the polyphasic data in this study, strain ZS3-33T represents a novel species of the genus Pseudorhodobacter, for which the name Pseudorhodobacter antarcticus sp. nov. is proposed. The type strain is ZS3-33T (=CGMCC 1.10836T= KCTC 23700T). An emended description of the genus Pseudorhodobacter Uchino et al. 2002 emend. Jung et al. 2012 is also proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30(T), was isolated from Antarctic intertidal sandy sediment. The strain grew at 4-35 °C (optimum, 25 °C) and in 0-25% (w/v) NaCl (optimum, 3.0-4.0%). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30(T) were summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(16:0), C(18:1)ω9c, C(16:1)ω9c, C(12:0) 3-OH and C(12:0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30(T) was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30(T) was affiliated with the genus Marinobacter. It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus Marinobacter, namely Marinobacter maritimus (98.3%), Marinobacter psychrophilus (98.1%) and Marinobacter goseongensis (97.1%), but the DNA-DNA relatedness values between strain ZS2-30(T) and the above three species were all lower than 45%. Moreover, strain ZS2-30(T) could be distinguished from closely related species of the genus Marinobacter by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30(T) is considered to represent a novel species in the genus Marinobacter, for which the name Marinobacter antarcticus sp. nov. is proposed. The type strain of Marinobacter antarcticus is ZS2-30(T) ( = CGMCC 1.10835(T) = KCTC 23684(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2011; 62(Pt 8):1838-44. · 2.11 Impact Factor
