Zhongxin Lu

Government of British Columbia, Canada, Vancouver, British Columbia, Canada

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Publications (6)9.48 Total impact

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    ABSTRACT: An indirect hemagglutination (IHA) test that could detect antibodies against Haemophilus parasuis was developed. The full-length cDNA sequence of the oligopeptide permease ABC transporter membrane protein (OppA) gene was cloned, and inserted into the prokaryotic expression vector pET-30a(+) to construct recombinant plasmid pET-30a-OppA. The recombinant OppA protein was expressed partly in soluble form in Escherichia coli BL21 (DE3) and then purified by Ni(2+) column. Furthermore, the recombinant OppA protein was used as an antigen to develop an IHA assay for detecting antibodies against H. parasuis. Results showed that this IHA test could detect species-specific antibodies against H. parasuis. Compared with currently available ELISA, the IHA test had a sensitivity of 85.0% and a specificity of 95.0%. The overall agreement between these two methods was 90.0%. The developed IHA test was used to evaluate the seroprevalence of H. parasuis in Hubei Province, China. The H. parasuis seroprevalence rate ranged from 5.5% to 26.2% in 325 tested clinical serum samples that were collected from three different pig farms in Hubei Province, China. The IHA test developed in this study will greatly contribute to the epidemiological surveys and immunization surveillance of H. parasuis. Copyright © 2015. Published by Elsevier B.V.
    Acta tropica 04/2015; 148. DOI:10.1016/j.actatropica.2015.04.009 · 2.27 Impact Factor
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    ABSTRACT: Mycoplasma capricolum sub sp. capripneumoniae (Mccp) is the causative agent of contagious capripleuropneumonia (CCBB) in goats. To develop more effective vaccines against CCBB, three protein sequences were optimized, in terms of codon usage bias, GC content, restriction enzymes, CIS-acting elements and repeat sequences, which are critical for efficient gene expression. These three proteins, respectively are the pyruvate dehydrogenase El component alpha subunit (BDHA), pyruvate dehydrogenase complex El component beta subunit (BDHB) and dihydrolip oy lysine- residue acetyltransferase components of the pyruvate dehydrogenase complex (BDHC). The three optimized genes were expressed in E. coli and the relevant proteins were analyzed further by in vivo expression detection and antibody detection. The results showed the following: (1) A significantly higher level of serum antibodies against the three proteins were detected with convalescent sera and immunized sera, which demonstrated that the three proteins were expressed in vivo. (2) Goats antisera tests against the three proteins showed that they could all elicit humoral immunity with potential antigenicity and reactogenicity. (3) The results suggested that these three proteins (BDHA, BDHB and BDHC) have strong potential as vaccine candidates.
    Asian Journal of Animal and Veterinary Advances 05/2013; 8(5):723-731. DOI:10.3923/ajava.2013.723.731 · 0.87 Impact Factor
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    ABSTRACT: Mycoplasma capricolum subsp. capripneumoniae is the causative agent of contagious caprine pleuropneumonia, a devastating disease of goats listed by the World Organization for Animal Health. Here we report the first complete genome sequence of this organism (strain M1601, a clinically isolated strain from China).
    Journal of bacteriology 11/2011; 193(21):6098-9. DOI:10.1128/JB.05980-11 · 2.81 Impact Factor
  • Jian Xu · Yuefeng Chu · Pengcheng Gao · Ping Zhao · Ying He · Genqiang Yan · Zhongxin Lu ·
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    ABSTRACT: To study the heterogeneity and immunogenic variability among Mycoplasma ovipneumoniae (M. ovipneumoniae) isolates from different regions of China. The heterogeneity of 17 strains of M. ovipneumoniae isolated from 8 regions of China was studied by the amplified fragment length polymorphism (AFLP) and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The software NTsys-2. 10e was used to analyze the profiles obtained from the AFLP and SDS-PAGE. The proteins reacted with the antiserum against M. ovipneumoniae type strain Y98 were then detected by Western-blot. Seventeen strains of M. ovipneumoniae were divided into 8 AFLP groups based on the source regions when the coefficient was 0.78. They were also divided into 8 SDS-PAGE groups based on the source regions when the coefficient was 0.85. A total of 6 immunogenic proteins were detected within 8 strains of M. ovipneumoniae, and their molecular weights were 105 kDa, 83 kDa, 65 kDa, 42 kDa, 40 kDa or 26 kDa, respectively. Interestingly, the 83 kDa and 40 kDa proteins were conserved in all the 8 isolates. M. ovipneumoniae isolates from some regions of China were genetically different, but the 83 kDa and 40 kDa antigenic proteins were conserved among the tested isolates. This study can provide some insights for the diagnosis and vaccine development of the disease caused by M. ovipneumoniae.
    ACTA MICROBIOLOGICA SINICA 10/2011; 51(10):1421-6.
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    Yuefeng Chu · Xinmin Yan · Pengcheng Gao · Ping Zhao · Ying He · Jixing Liu · Zhongxin Lu ·
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    ABSTRACT: The current study investigated an outbreak of mixed infection with Goatpox virus (GTPV), Orf virus (ORFV), and Mycoplasma capricolum subsp. capripneumoniae (MCCP) that occurred on a Chinese goat farm, with a case fatality rate of 60.2%. The observed clinical signs were ecthyma and accelerated respiration with frequent coughing. Specific fragments of the p32 gene of GTPV, B2L gene of ORFV, and 16S ribosomal RNA gene of MCCP were synchronously amplified by polymerase chain reaction (PCR) from the tissues of 12 dead goats. The PCR products were cloned, sequenced, and aligned with related reference sequences in GenBank for further identification of the pathogens. The present study reports a mixed infection with GTPV, ORFV, and MCCP in goats.
    Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 07/2011; 23(4):786-9. DOI:10.1177/1040638711407883 · 1.35 Impact Factor
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    ABSTRACT: Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants. In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene. The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.
    Virology Journal 04/2010; 7(1):78. DOI:10.1186/1743-422X-7-78 · 2.18 Impact Factor

Publication Stats

33 Citations
9.48 Total Impact Points


  • 2011
    • Government of British Columbia, Canada
      Vancouver, British Columbia, Canada
  • 2010-2011
    • Lanzhou Veterinary Research Institute
      Kao-lan-hsien, Gansu Sheng, China