Xinjian Zhang

Publications (2)3.37 Total impact

• Article: Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillussubtilis chitinase gene chi113

  Xinjian Zhang, Yujie Huang, Paul R. Harvey, Yan Ren, Guangzhi Zhang, Hongzi Zhou, Hetong Yang
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  ABSTRACT: Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N2-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P<0.05) increased in vitro inhibition of the plant pathogenic fungi Rhizoctonia solani, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, Bipolaris sorokiniana, Verticillium dahliae and Gaeumannomyces graminis var. tritici compared with P418. In planta disease suppression assays indicated that P418-37 significantly (P<0.05) enhanced suppression of wheat sheath blight (R. cerealis), cotton Fusarium wilt (F. oxysporium f.sp. vasinfectum) and tomato gray mould (Botrytis cinerea), relative to the wild type. KeywordsBurkholderia vietnamiensis– Chitinase –Disease suppression–Transposon
  Biotechnology Letters 04/2012; 34(2):287-293. · 1.68 Impact Factor
• Article: Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113.

  Xinjian Zhang, Yujie Huang, Paul R Harvey, Yan Ren, Guangzhi Zhang, Hongzi Zhou, Hetong Yang
  [show abstract] [hide abstract]
  ABSTRACT: Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) increased in vitro inhibition of the plant pathogenic fungi Rhizoctonia solani, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, Bipolaris sorokiniana, Verticillium dahliae and Gaeumannomyces graminis var. tritici compared with P418. In planta disease suppression assays indicated that P418-37 significantly (P < 0.05) enhanced suppression of wheat sheath blight (R. cerealis), cotton Fusarium wilt (F. oxysporium f.sp. vasinfectum) and tomato gray mould (Botrytis cinerea), relative to the wild type.
  Biotechnology Letters 02/2012; 34(2):287-93. · 1.68 Impact Factor