Publications (2)4.86 Total impact
-
Article: Importance of observation interval in two-dimensional video analysis of individual diatom cells.
[show abstract] [hide abstract]
ABSTRACT: The effect of the observation interval on two-dimensional trajectory analysis of motility of individual diatom cells of Navicula sp. was studied by comparing thinned-out observation data. The trajectory of cell movement was visualized accurately even after thinning the data interval. However, the analysis of velocity fluctuations of individual cells was found to be significantly affected by the data interval. Reproducibility of the results was guaranteed by analyzing many independent cells. In addition, comparison between automatic and manual determination of cell positions proved that automatic determination was a reliable process. Our data indicated that two-dimensional trajectory analysis using a computer can be a powerful technique to study diatom locomotion.Biophysics of Structure and Mechanism 04/2012; 41(6):545-50. · 2.44 Impact Factor -
Article: Two-dimensional trajectory analysis of the diatom Navicula sp. using a micro chamber.
[show abstract] [hide abstract]
ABSTRACT: We describe a trajectory analysis of diatom cell locomotion by combining a micro chamber and two-dimensional position coordinate analysis. By shutting cells in a micro chamber, continuous microscopic observation of Navicula sp. cells was possible. The trajectory of each cell was visualized once every second by using position coordinate analysis although time resolution of previous papers were range of minutes. Our data revealed frequent change of movement direction. Furthermore, the correlation between the distances moved, the velocity, and the acceleration of the cells was discussed in detail.Journal of microbiological methods 09/2011; 87(3):316-9. · 2.43 Impact Factor
