Nicolas A Giraldo

Los Andes University (Colombia), Bogotá, Bogota D.C., Colombia

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Publications (3)9.45 Total impact

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    ABSTRACT: Chronic persistent infections have been associated with T lymphocytes functional impairment. The aim of this study was to compare the activation status, the proliferative potential and the expression of CD28 and CD3ζ chain on T lymphocytes between chronic chagasic patients and uninfected controls. Forty-two chronic chagasic patients, 28 healthy individuals and 32 non-chagasic cardiomyopathy donors were included. Peripheral blood was marked for CD3, CD4, CD8, HLA-DR, CD28, CD38 and intracellular CD3ζ. Peripheral blood mononuclear cells were stained with carboxyfluorescein diacetate succinimidylester and incubated with T. cruzi lysate or phytohemagglutinin for five days. Cells from 3 healthy controls were incubated with T. cruzi trypomastigotes separated with transwells; and the expression of CD3ζ chain and proliferation index was determined. Heart-infiltrating cells from two chronic chagasic patients were tested for the aforementioned cellular markers. Chagasic patients displayed higher frequencies of CD4+/HLA-DR+/CD38+ (8.1%±6.1) and CD8+/HLA-DR+/CD38+ (19.8±8.9) T cells in comparison with healthy (1.6±1.0; 10.6±8.0) and non-chagasic cardiomyopathy donors (2.9±2.9; 5.8±6.8). Furthermore, the percentage of CD4+ activated T cells was higher in chagasic patients with cardiac involvement. CD8+ T cells proliferation index in chagasic donors (1.7±0.3) was lower when compared with healthy (2.3±0.3) and non-chagasic cardiomyopathy individuals (3.1±1.1). The frequencies of CD4+/CD28+ and CD8+/CD28+ T cells, as well as the CD3ζ(bright)/CD3ζ(dim)% ratios in CD4+ and CD8+ were lower in chagasic patients when compared with both control groups. The CD3ζ(bright)/CD3ζ(dim)% ratio and proliferative indexes for CD4+ and CD8+ T lymphocytes decreased gradually in those cells cultivated with parasites and displayed lower values than those incubated with medium alone. Finally, heart-infiltrating T cells from two T. cruzi infected patients also expressed activation markers and down-regulate CD28 and CD3ζ. CD8+ T lymphocytes from chagasic donors displayed reduced proliferative capacity, which might be associated with CD3ζ down-regulation and diminished CD28 expression on CD4 T cells.
    PLoS Neglected Tropical Diseases 01/2013; 7(1):e2038. · 4.57 Impact Factor
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    ABSTRACT: CD4+/CD8+ double positive (DP) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases. In chronic viral infections, this cell subset has effector memory phenotype and displays antigen specificity. No previous studies of double positive T cells in parasite infections have been carried out. Seventeen chronic chagasic patients (7 asymptomatic and 10 symptomatic) and 24 non-infected donors, including 12 healthy and 12 with non-chagasic cardiomyopathy donors were analyzed. Peripheral blood was stained for CD3, CD4, CD8, HLA-DR and CD38, and lymphocytes for intracellular perforin. Antigen specificity was assessed using HLA*A2 tetramers loaded with T. cruzi K1 or influenza virus epitopes. Surface expression of CD107 and intracellular IFN-γ production were determined in K1-specific DP T cells from 11 chagasic donors. Heart tissue from a chronic chagasic patient was stained for both CD8 and CD4 by immunochemistry. Chagasic patients showed higher frequencies of DP T cells (2.1% ± 0.9) compared with healthy (1.1% ± 0.5) and non-chagasic cardiomyopathy (1.2% ± 0.4) donors. DP T cells from Chagasic patients also expressed more HLA-DR, CD38 and perforin and had higher frequencies of T. cruzi K1-specific cells. IFN-γ production in K1-specific cells was higher in asymptomatic patients after polyclonal stimulation, while these cells tended to degranulate more in symptomatic donors. Immunochemistry revealed that double positive T cells infiltrate the cardiac tissue of a chagasic donor. Chagasic patients have higher percentages of circulating double positive T cells expressing activation markers, potential effector molecules and greater class I antigenic specificity against T. cruzi. Although K1 tetramer positive DP T cell produced little IFN-γ, they displayed degranulation activity that was increased in symptomatic patients. Moreover, K1-specific DP T cells can migrate to the heart tissue.
    PLoS Neglected Tropical Diseases 08/2011; 5(8):e1294. · 4.57 Impact Factor
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    Nicolas Giraldo, John M. Gonzalez
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    ABSTRACT: Objetivo. Se realizó una búsqueda sistemática de la literatura científica con el objetivo de conocer el número de artículos colombianos publicados con el uso de citometría de flujo, sus aplicaciones y el aporte a la literatura mundial sobre el tema.Método. Se utilizaron las bases de datos electrónicas PubMed, LILACS e ISI Web of Knowledge, y se buscaron los términos “flow cytometry” and “Colombia” o “citometría de flujo” y “Colombia”. Se incluyeron trabajos de investigación experimentales que usaron la citometría de flujo y cuyo primero o último autor pertenecieran a grupos de investigación colombianos.Resultados. Se encontraron 74 artículos que cumplían los criterios de inclusión, publicados desde agosto de 1997 hasta marzo de 2010. La gran mayoría de los trabajos pertenecía al área biomédica y en 54% se aplicó al estudio de enfermedades infecciosas. El número de publicaciones por año aumentó desde 1997 hasta 2004, cuando se presentó un pico de 11 artículos. En total, se encontraron 27 grupos de investigación, pertenecientes a 13 instituciones. El 39,2% (n=29) de los trabajos fueron publicados por grupos de investigación de la Universidad de Antioquia. El tipo de aplicación de la citometría de flujo más usada fue el análisis de inmunofenotipo (58,1%).Conclusiones. Para el periodo comprendido entre agosto de 1997 y marzo de 2010, PubMed reportó 76.312 publicaciones relacionadas con la citometría de flujo. Por consiguiente, el aporte de las publicaciones colombianas a la literatura mundial es tan sólo de 0,096%.
    Biomédica: revista del Instituto Nacional de Salud 03/2010; 30(S1):32. · 0.32 Impact Factor