Mitsuru Yokoyama

University of Occupational and Environmental Health, Kitakyūshū, Fukuoka, Japan

Are you Mitsuru Yokoyama?

Claim your profile

Publications (2)8.19 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Fullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
    Journal of Hazardous Materials 08/2011; 194:324-30. · 4.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In recent years, forensic scientists showed that an individual's genetic profile can be retrieved from touched objects. Degraded DNA is believed to originate from epidermal cells and to be responsible for this phenomenon, yet the mechanism has not been confirmed. In the present study, we carried out a morphological and immunohistochemical investigation of nuclear DNA in differentiating keratinocytes in the skin and also a genetic analysis of DNA on swabs of human skin. Immunoelectron microscope analysis showed that single-stranded DNA was found both in the cornified layer of the skin and in swabs. Real-time-PCR assay proved that the DNA in the swabs was derived from the human DNA. Electron microscopic analysis of shadow-cast showed the presence of small DNA fragments in the swabs. It is conceivable that these DNA fragments on touched objects may originate from the epidermal cells of the cornified layer that are constantly sloughed off and leave for skin surface with sweat.
    Forensic Science International: Genetics 01/2009; 3(1):32-6. · 3.86 Impact Factor

Publication Stats

13 Citations
8.19 Total Impact Points


  • 2009–2011
    • University of Occupational and Environmental Health
      • School of Medicine
      Kitakyūshū, Fukuoka, Japan