Mitsuru Yokoyama

University of Occupational and Environmental Health, Kitakyūshū, Fukuoka, Japan

Are you Mitsuru Yokoyama?

Claim your profile

Publications (1)3.86 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In recent years, forensic scientists showed that an individual's genetic profile can be retrieved from touched objects. Degraded DNA is believed to originate from epidermal cells and to be responsible for this phenomenon, yet the mechanism has not been confirmed. In the present study, we carried out a morphological and immunohistochemical investigation of nuclear DNA in differentiating keratinocytes in the skin and also a genetic analysis of DNA on swabs of human skin. Immunoelectron microscope analysis showed that single-stranded DNA was found both in the cornified layer of the skin and in swabs. Real-time-PCR assay proved that the DNA in the swabs was derived from the human DNA. Electron microscopic analysis of shadow-cast showed the presence of small DNA fragments in the swabs. It is conceivable that these DNA fragments on touched objects may originate from the epidermal cells of the cornified layer that are constantly sloughed off and leave for skin surface with sweat.
    Forensic Science International: Genetics 01/2009; 3(1):32-6. · 3.86 Impact Factor

Publication Stats

10 Citations
3.86 Total Impact Points

Institutions

  • 2009
    • University of Occupational and Environmental Health
      • School of Medicine
      Kitakyūshū, Fukuoka, Japan