M Kmeťová

Publications (3)2.03 Total impact

• Article: Detection ofpap-,sfa- andafa-specific DNA sequences inEscherichia coli strains isolated from extraintestinal material

  E. Bogyiová, M. Kmeťová, E. Biroš, L. Siegfried
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  ABSTRACT: P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence ofEscherichia coli strains to extraintestinal host-cell surface. Detection ofpap-,sfa- andafa-specific sequences performed by PCR revealed 74%pap +, 65%sfa +, and 8.3%afa + strains in a group of 84 extraintestialE. coli isolates. Detection in a group of fecal strains showed 29%pap +, 21%sfa + and 4%afa + strains.pap together withsfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization ofpap-andsfa-operons on a common pathogenicity island. The occurrence ofafa-specific sequence among 56 urine strains was 11%, although noafa + strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinalE. coli strains.
  Folia Microbiologica 04/2012; 47(6):723-726. · 0.68 Impact Factor
• Article: Enterovirulent E. coli in inflammatory and noninflammatory bowel diseases

  K. Čurová, M. Kmeťová, M. Sabol, L. Gombošová, I. Lazúrová, L. Siegfried
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  ABSTRACT: We determined the incidence of enterovirulent E. coli (EVEC; which can to cause gastrointestinal infections) in strains isolated from patients with both of the major inflammatory bowel diseases (IBD), Crohn’s disease (CD) and ulcerative colitis (UC) and from patients with noninflammatory bowel diseases (nonIBD). Cell detachment E. coli (CDEC) were detected in 14 % of all strains. A significant difference in the presence of CDEC was found between the groups of strains isolated from UC (24.1 %), nonIBD (11.9 %) and CD (4.7 %). Enteroaggregative E. coli (EAggEC) were detected in 2.5 %, typical enteropathogenic strains (EPEC) in 1.3 % and enterotoxigenic ones (ETEC) in 1.5 %. Enteroinvasive (EIEC) and shigatoxin producing E. coli (STEC) were not detected. Some strains showed a high invasion level in gentamicin-protection assay. These strains could therefore belong to adherent-invasive E. coli (AIEC) because they are free of genes encoding invasins (ipaH, ial) and are equipped with fimA gene. However, complete characterization of these strains and their classification as AIEC will require further tests.
  Folia Microbiologica 04/2012; 54(1):81-86. · 0.68 Impact Factor
• Source

  Available from: PubMed Central

  Article: Genes of intestinal Escherichia coli and their relation to the inflammatory activity in patients with ulcerative colitis and Crohn's disease.

  Laura Gombošová, I Lazúrová, M Zakuciová, K Curová, M Kmeťová, D Petrášová, L Siegfried
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  ABSTRACT: Escherichia coli gene fimA was the most frequent gene that occurred in the intestine of all investigated groups. All subjects with fimA gene had significantly higher values of tumor necrosis factor alpha (TNF-α) and CRP than those with other E. coli genes. There was also a tendency to increased serum interleukin (IL)-6 levels in patients carrying the fimA gene; however, no relation was observed to serum IL-8 and IL-10. Patients with Crohn's disease had significantly higher IL-6 than those with ulcerative colitis (UC) and controls. The highest levels of TNF-α were detected in the UC group. There were no significant differences in serum IL-8 and IL-10 between all three groups. The presence of E. coli gene fimA in the large bowel of patients with IBD is related to the immunological activity of the disease which may be important from the aspect of therapeutical strategy.
  Folia Microbiologica 08/2011; 56(5):367-72. · 0.68 Impact Factor