Jan Pietruski

Philipps University of Marburg, Marburg, Hesse, Germany

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Publications (17)20.7 Total impact

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    ABSTRACT: The use of antibacterial photodynamic therapy (aPDT) additionally to scaling and root planing (SRP) has been shown to positively influence the clinical outcomes. However, at present, it is unknown to what extent aPDT may represent a potential alternative to the use of systemic antibiotics in nonsurgical periodontal therapy in patients with aggressive periodontitis (AP). The aim of this study was to evaluate the outcomes following nonsurgical periodontal therapy and additional use of either aPDT or amoxicillin and metronidazole (AB) in patients with AP. Thirty-six patients with AP displaying at least three sites with pocket depth (PD) ≥6 mm were treated with SRP and either systemic administration of AB for 7 days or with two episodes of aPDT. The following clinical parameters were evaluated at baseline and at 6 months: plaque index (PI), bleeding on probing (BOP), PD, gingival recession (GR) and clinical attachment level (CAL). Thirty-five patients have completed the 6-month evaluation. At 6 months, mean PD was statistically significantly reduced in both groups (from 5.0 ± 0.8 to 3.0 ± 0.6 mm with AB and from 5.1 ± 0.5 to 3.9 ± 0.8 mm with aPDT (p < 0.001)). AB yielded statistically significantly higher improvements in the primary outcome parameter PD (p < 0.001) when compared to aPDT. The number of pockets ≥7 mm was reduced from 141 to 3 after AB (p < 0.001) and from 137 to 45 after aPDT (p = 0.03). Both therapies resulted in statistically significant reductions in all parameters compared to baseline. While both treatments resulted in statistically significant clinical improvements, AB showed statistically significantly higher PD reduction and lower number of pockets ≥7 mm compared to aPDT. In patients with AP, the two times application of aPDT in conjunction with nonsurgical periodontal therapy cannot be considered an alternative to the systemic use of amoxicillin and metronidazole.
    Clinical Oral Investigations 02/2014; · 2.20 Impact Factor
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    ABSTRACT: The aim of the study was clinical evaluation of photodynamic therapy efficacy in the treatment of oral leukoplakia lesions. Twenty three consecutive patients aged 21-79 were included to the study. In all patients 44 homogeneous, flat leukoplakia lesions were clinically diagnosed and confirmed histopoathologically. Photodynamic therapy was performed with the use of Photolon(®) photosensitizer, containing 20% chlorine-e6 and 10% dimethyl sulfoxide and a semiconductor laser, with power up to 300mW and a wavelength of 660nm. Ten illumination sessions were conducted with the use of superficial light energy density of 90J/cm(2). At baseline the mean size of leukoplakia lesion was 6.5 cm(2)±5.10 while after photodynamic therapy 3cm(2)±2.99. Significant reduction (on average by 53.8%) of leukopakia lesions sizes was observed after therapy. Twelve (27.27%) lesions had been completely, 22 (50%) partially cured, although 10 (22.73%) lasted unchanged. The efficacy of PTD was comparable in women and men irrespective of age. There have been no adverse site effects during therapy noted. Within the limits of the study it can be concluded that photodynamic therapy with the use of Chlorine-e6 can lead to considerable reduction of oral leukoplakia lesions size thus may be useful in clinical practice. However there is a need of further studies on larger number of cases and longer follow up time.
    Photodiagnosis and photodynamic therapy 11/2013;
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    ABSTRACT: The puprose of this study was to evaluate retention force of conical double crowns in two material connections: gold casting alloy/gold casting alloy and gold casting alloy/gold electroforming alloy. 12 crown pairs of both material connections with the cone angles of 2°, 4° and 6° were made. Experiment of 10.000 in-and-out cycles was performed using a new device which allows the retentive force to be measured in continuous way without necessity of moving the samples to another device. It has been found that the higher the retentive force values, the lower the cone angle. Dispersion of the retention value was similar in both groups, but when cone angle was 2° or 4°, stability of retention force with the passage of time was higher in combinations with electroformed copings. The optimum solution was the cast alloy/cast alloy connection but only with cone angle 6°. However, retentive values seem to be too low to achieve proper retention of dentures.
    Acta of bioengineering and biomechanics / Wroclaw University of Technology 01/2013; 15(1):35-42. · 0.98 Impact Factor
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    ABSTRACT: Summary The aim of this randomized, controlled clinical study was to compare the short-term effects of nonsurgical periodontal therapy with the additional administration of systemic antibiotics (AB) and the same therapy with additional photodynamic therapy (PDT) in the treatment of patients with aggressive periodontitis (AP). Thirty-six patients with AP received full-mouth nonsurgical periodontal treatment (SRP) and were then randomly divided into two groups of 18 subjects each. Group AB received amoxicillin and metronidazole three times a day for 7 days. Group PDT received two applications of PDT on the day of SRP as well as at follow-up after 7 days. The following clinical parameters were measured at baseline and 3 months after therapy: plaque index (PLI), bleeding on probing (BOP), probing depth (PD), gingival recession (GR), and clinical attachment level (CAL). After 3 months, PD was significantly reduced in both groups (from 5.0 ± 0.8 mm to 3.2 ± 0.4 mm with AB, and 5.1± 0.5 mm to 4.0 ± 0.8 mm with PDT; both p < 0.001), while AB revealed significantly lower values compared to PDT (p = 0.001). In both groups, GR was not significantly changed. CAL was significantly reduced in both groups (PDT: 5.7±0.8 mm to 4.7±1.1 mm; p = 0.011; AB: 5.5 ±1.1 mm to 3.9± 1.0 mm; p < 0.001) and differed significantly between the groups (p = 0.025). The number of residual pockets (PD ≥4 mm) and positive BOP was reduced by AB from 961 to 377, and by PDT from 628 to 394. Pockets with PD ≥ 7 mm were reduced by AB from 141 to 7, and by PDT from 137 to 61. After 3 months, both treatments led to statistically significant clinical improvements. The systemic administration of antibiotics, however, resulted in significantly higher reduction of PD and a lower number of deep pockets compared to PDT.
    Schweizerische Monatsschrift für Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia / SSO 01/2013; 123(6):532-8.
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    ABSTRACT: The puprose of this study was to evaluate retention force of conical double crowns in two material connections: gold casting alloy/gold casting alloy and gold casting alloy/gold electroforming alloy. 12 crown pairs of both material connections with the cone angles of 2°, 4° and 6° were made. Experiment of 10.000 in-and-out cycles was performed using a new device which allows the retentive force to be measured in continuous way without necessity of moving the samples to another device. It has been found that the higher the retentive force values, the lower the cone angle. Dispersion of the retention value was similar in both groups, but when cone angle was 2° or 4°, stability of retention force with the passage of time was higher in combinations with electroformed copings. The optimum solution was the cast alloy/cast alloy connection but only with cone angle 6°. However, retentive values seem to be too low to achieve proper retention of dentures.
    Acta of bioengineering and biomechanics / Wroclaw University of Technology 01/2013; 15(1):35-42. · 0.98 Impact Factor
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    ABSTRACT: This study presents the results of a long-term clinical evaluation of conical crown-retained dentures fabricated using different technologies. Four different material connections between the outer and inner crowns were used: cast gold/cast gold, cast gold/electroforming, nonprecious alloy/electroforming, and titanium abutment/electroforming. Technical failures and retention values were assessed. The best clinical outcome was found with dentures in which both crowns were cast from gold alloy. The most frequent technical failures were observed in restorations with electroformed outer crowns. Better clinical outcomes were noted when the electroformed outer crowns were used in dentures retained by implants as compared to dentures on natural dentition.
    The International journal of periodontics & restorative dentistry 08/2012; 32(4):467-75. · 1.01 Impact Factor
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    ABSTRACT: The study objective was clinical assessment of the efficacy of photodynamic therapy (PDT) in the treatment of oral lichen planus (OLP). There were 23 patients aged 31-82 included in the study with oral lichen planus diagnosed clinically and histopathologically. In all patients photodynamic therapy was performed with the use of chlorin e6 (Photolon(®)), containing 20 % chlorin e6 and 10 % dimethyl sulfoxide as a photosensitizer. PDT was performed using a semiconductor laser, with power up to 300 mW and a wavelength of 660 nm. A series of illumination sessions was conducted with the use of superficial light energy density of 90 J/cm(2). Changes of lesion size were monitored at one, two, five, and ten PDT appointments from the series of ten according to the authors' own method. The sizes of clinical OLP lesions exposed to PDT were reduced significantly (on average by 55 %). The best effects were observed for the lesions on the lining mucosa (57.6 %). The therapy was statistically significantly less effective when masticatory mucosa was affected (reduction, 30.0 %). Due to substantial efficacy and noninvasiveness, PDT can be useful in the treatment of OLP lesions.
    Lasers in Medical Science 07/2012; · 2.42 Impact Factor
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    ABSTRACT: The aim of this study has been to compare the clinical and radiographic outcome of periodontal intrabony defect treatment by open flap debridement alone or in combination with nanocrystalline hydroxyapatite bone substitute application. Thirty patients diagnosed with advanced periodontits were divided into two groups: the control group (OFD), in which an open flap debridement procedure was performed and the test group (OFD+NHA), in which defects were additionally filled with nanocrystalline hydroxyapatite bone substitute material. Plaque index (PI), gingival index (GI), bleeding on probing (BOP), pocket depth (PD), gingival recession (GR) and clinical attachment level (CAL) were measured prior to, then 6 and 12months following treatment. Radiographic depth and width of defects were also evaluated. There were no differences in any clinical and radiographic parameters between the examined groups prior to treatment. After treatment, BOP, GI, PD, CAL, radiographic depth and width parameter values improved statistically significantly in both groups. The PI value did not change, but the GR value increased significantly after treatment. There were no statistical differences in evaluated parameters between OFD and OFD+NHA groups 6 and 12months after treatment. Within the limits of the study, it can be concluded that the additional use of nanocrystalline hydroxyapatite bone substitute material after open flap procedure does not improve clinical and radiographic treatment outcome.
    Annals of anatomy = Anatomischer Anzeiger: official organ of the Anatomische Gesellschaft 06/2012; · 1.96 Impact Factor
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    ABSTRACT: The enzymatic profile of lysosomal exoglycosidases in middle ear cholesteatoma has not been well known. The assessment of glycoconjugate catabolism may contribute to a better understanding of cholesteatoma pathogenesis. The study aim was to evaluate catabolic processes of glycoproteins, glycolipids, and proteoglycans in cholesteatoma through outlining the concentration of N-acetyl-β-hexosaminidase (HEX), β-glucuronidase (GLUC), and β-galactosidase (GAL) activity as well as in serum of cholesteatoma patients and healthy volunteers. Acquired cholesteatomas (n = 25) and normal retroauricular skin specimens (n = 25) were taken during surgery as well as serum from cholesteatoma patients and healthy volunteers. HEX, GAL, and GLUC activity was assessed on basis of p-nitrophenol release from derivatives of the substrate (HEX: N-acetylglucosamine i N-acetylgalactosamine, GAL from galactose, and GLUC from glucuronide). The mean concentration of activity of HEX 1142.39 pKat/ml, GAL 8.90 pKat/ml, and GLUC 14.06 pKat/ml was significantly higher compared with the concentration of enzyme activity in normal tissue: HEX 267.65 pKat/ml, GAL 3.44 pKat/ml, and GLUC 3.90 pKat/ml. In the serum of cholesteatoma patients, the mean concentration of enzyme activities were as follows: HEX 641.62 pKat/ml, GAL 4.55 pKat/ml, and GLUC 12.80 pKat/ml and were significantly higher compared with the concentration of HEX activity (215.75 pKat/ml), GAL (1.89 pKat/ml), and GLUC (5.51 pKat/ml) in the serum of the healthy control group. In cholesteatoma compared with the normal tissue, there is an increase of the glycoconjugate catabolism due to significantly higher concentration of HEX, GAL, and GLUC activity in cholesteatoma. Cholesteatoma causes systemic reaction due to the increase of HEX, GAL, and GLUC activity in patient serum.
    Otology & neurotology: official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 06/2012; 33(4):596-603. · 1.44 Impact Factor
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    ABSTRACT: Lysosomal exoglycosidases, such as α-mannosidases (MAN) and β-galactosidases (GAL), are found in different glycoside hydrolase sequence-based families. Considerable research has proved plays the role of MAN, which play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer. Therefore the study aim was to investigate the activities of MAN and GAL in larynx cancer compared to controls. Larynx cancer (n = 21) and normal healthy tissue (n = 21) were collected from patients during total laryngectomy. A biopsy of macroscopically healthy tissue in the area of the lower 1/3 of omohyoid muscle was taken for frozen sections in each case and these served as controls. The release of p-nitrophenol from p-nitrophenol derivatives of MAN and GAL was used. In all specimens we observed significantly higher activity of investigated enzymes in larynx cancer compared with controls. The mean release of MAN from activated cells was 3.702 ±1.3245 nkat/g wet tissue compared to controls (1.614 ±0.8220 nkat/g wet tissue). The mean release of GAL from the activated cells was 3.383 ±2.1980 nkat/g wet tissue compared to controls (2.137 ±1.3685 nkat/g wet tissue). Differences in observed activity were statistically significant. The present data indicate that MAN and GAL are significantly and consistently elevated in larynx cancer growth. It also means that catabolic reactions involving glycoproteins, glycolipids and proteoglycans may play a role in larynx cancer. Further research should also evaluate the relative importance of these particular exoglycosidases in indicating the progress of the disease in considering the spectrum of identified marker mediators.
    Contemporary Oncology / Wspólczesna Onkologia 01/2012; 16(2):154-158. · 0.22 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the 4-year clinical outcomes following regenerative surgery in intrabony defects with either EMD + BCP or EMD. Twenty-four patients with advanced chronic periodontitis, displaying one-, two-, or three-walled intrabony defect with a probing depth of at least 6 mm, were randomly treated with either EMD + BCP (test) or EMD alone (control). The following clinical parameters were evaluated at baseline, at 1 year and at 4 years after regenerative surgery: plaque index, gingival index, bleeding on probing, probing depth, gingival recession, and clinical attachment level (CAL). The primary outcome variable was CAL. No differences in any of the investigated parameters were observed at baseline between the two groups. The test group demonstrated a mean CAL change from from 10.8 ± 1.6 mm to 7.4 ± 1.6 mm (p < 0.001) and to 7.6 ± 1.7 mm (p < 0.001) at 1 and 4 years, respectively. In the control group, mean CAL changed from 10.4 ± 1.3 at baseline to 6.9 ± 1.0 mm (p < 0.001) at 1 year and 7.2 ± 1.2 mm (p < 0.001) at 4 years. At 4 years, two defects in the test group and three defects in the control group have lost 1 mm of the CAL gained at 1 year. Compared to baseline, at 4 years, a CAL gain of ≥3 mm was measured in 67% of the defects (i.e., in 8 out of 12) in the test group and in 75% of the defects (i.e., in 9 out of 12) in the control group. There were no statistically significant differences in any of the investigated parameters at 1 and at 4 years between the two groups. Within their limits, the present results indicate that: (a) the clinical improvements obtained with both treatments can be maintained over a period of 4 years, and (b) in two- and three-walled intrabony defects, the addition of BCP did not additionally improve the outcomes obtained with EMD alone. In two- and three-walled intrabony defects, the combination of EMD + BCP did not show any advantage over the use of EMD alone.
    Clinical Oral Investigations 09/2011; 16(4):1191-7. · 2.20 Impact Factor
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    ABSTRACT: A comparison of the clinical status and salivary MMP levels after SRP alone or with ozonotherapy in patients with aggressive and chronic periodontitis. The study was performed in 52 generally healthy subjects with chronic or aggressive periodontitis. Group CP-S consisted of 12 patients with chronic periodontitis, who underwent scaling and root planing (SRP). In group CP-O there were 25 patients with chronic periodontitis who additionaly to SRP underwent ozonotherapy. The same therapy was performed in group AP, containing 15 patients with aggressive periodontitis. Plaque index, approximal plaque index, bleeding on probing, sulcus bleeding index, probing pocket depth and clinical attachment loss were measured at baseline, at two weeks and two months post-therapy. The levels of MMP-1, MMP-8 and MMP-9 were estimated in non-stimulated saliva with an ELISA method. All the clinical parameters assessed in the study groups were reduced after treatment. SRP with additional ozonotherapy provided an increase in MMP levels in patients with chronic periodontitis and a reduction in MMP levels in patients with aggressive periodontitis. SRP followed by ozonotherapy does not lead to further improvement in clinical periodontal parameters in patients with AP and CP.
    Advances in Medical Sciences 12/2010; 55(2):297-307. · 0.80 Impact Factor
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    ABSTRACT: Assessment of the effect of treatment with aprotinin-containing drug on the clinical status of the periodontal tissue and on the concentrations of metalloproteinases released in the course of periodontitis (MMP-1, MMP-2) as well as their tissue inhibitors (TIMP-1 and TIMP-2) in the saliva of patients with chronic periodontitis (CP). The study involved 25 subjects with CP (39-68 years), including 16 women and 9 men. The patients were prescribed aprotinin preparation to be taken for 2 weeks. The control group (C) involved 14 healthy subjects (41-65 years), including 10 women and 4 men. Two periodontal indices were assessed: the approximal plaque index (API) and bleeding on probing index (BOP). Periodontal pocket depth and clinical attachment level were also evaluated. The concentrations of MMP-1 and MMP-2 as well as TIMP-1 and TIMP-2 were determined by the ELISA method. The mean salivary MMP-1 concentration in patients with CP was significantly higher before and after treatment, as compared to healthy subjects. The mean salivary MMP-2 concentration in CP patients at baseline was also higher as compared to the C group and increased after treatment. The mean salivary TIMP-1 and TIMP-2 concentration in CP patients was higher as compared to C group and increased after treatment. Since the mean MMPs levels were found to be growing it can be assumed that aprotinin has no significant effect on the regulation of MMPs in the saliva of CP patients. It thus seems that aprotinin application after scaling has no additional therapeutic effect.
    Advances in Medical Sciences 09/2009; 54(2):239-46. · 0.80 Impact Factor
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    ABSTRACT: The aim of the study was the clinical assessment of the periodontium in patients with aggressive periodontitis (AP) after treatment with doxycycline hyclate. Moreover, an attempt was made to evaluate the effect of the treatment on the salivary concentrations of beta-glucuronidase, HEX, HEX A and HEX B in AP patients. Sixteen patients with aggressive periodontitis, aged 28-45 years, were enrolled in the study. The patients were treated with a doxycycline hyclate preparation (Periostat) for 2 months at a dose of 20 mg twice a day. The clinical examination was performed twice, directly prior to pharmacological treatment and after its termination. The following clinical parameters were evaluated: the plaque index (PI), the sulcus bleeding index (SBI), the pocket probing depth (PPD) and the clinical attachment level (CAL). Biochemical determination of beta-glucuronidase, HEX, HEX A and HEX B concentrations in non-stimulated saliva was performed before and after treatment. In AP patients, the values of PI, SBI and CAL before and after treatment were comparable. The mean pocket probing depth before treatment was 3.5 mm, which decreased significantly after treatment (3.2 mm). The values expressed as pKat/kg protein for specific enzymatic activities of HEX, HEX A, HEX B and beta-glucuronidase in the saliva of AP patients before and after doxycycline treatment were similar. A 2-month treatment with doxycycline is too short to obtain clinical changes. Although the assessment of the activity of such enzymes as beta-glucuronidase, HEX, HEX A and HEX B in the saliva of AP patients allows detection of periodontal inflammation, it cannot be used to determine the risk of its development and therefore has no practical significance.
    Advances in Medical Sciences 02/2006; 51 Suppl 1:158-61. · 0.96 Impact Factor
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    ABSTRACT: Chlorhexidine gluconate is a relatively commonly used chemotherapeutic in the treatment of periodontitis (P), exhibiting antimicrobial capabilities against Gram-negative and Gram-positive bacteria, and fungi. This compound is a component of various preparations for topical use in the form of solutions for mouthrinsing or peri-irrigation, gels, varnishes, chips and even chewing gums. The aim of the study was the clinical evaluation of periodontium after treatment with one of the drugs containing chlorhexidine gluconate (Corsodyl) as compared to professional tooth cleaning in patients with chronic periodontitis. MATERAL AND METHODS: Forty subjects enrolled in the study were divided into four groups, 10 in each group, according to the mode of treatment (Corsodyl rinse, Corsodyl gel, Corsodyl gel + surgical dressing, scaling). The greatest differences between baseline and follow-up examinations were observed in the group where surgical dressing was applied in addition to Corsodyl gel and in the group treated with scaling. Chlorhexidine gluconate should be more frequently used as a drug adjunct to classic periodontal therapy, especially in the forms allowing its direct application to the periodontal pockets.
    Advances in Medical Sciences 02/2006; 51 Suppl 1:162-5. · 0.96 Impact Factor
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    M Pietruska, J Zak, J Pietruski, J Wysocka
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    ABSTRACT: Lipopolysaccharides (LPS), a major component of the cell membrane of gram-negative bacteria, are the main stimulants of the host immune response, initiating inflammatory changes and responsible for periodontal tissue destruction. The mCD14, which is found primarily on monocytes and macrophages, is the key membranous receptor involved in LPS binding. CD14 is also present in the serum as a soluble form (sCD14) released due to shedding from monocytes. The aim of the study was to assess CD14 expression on peripheral blood monocytes in patients with generalized aggressive periodontitis (GAP). The level of sCD14 was also determined in the serum of GAP patients. The study group consisted of 16 patients with generalized aggressive periodontitis, the control group had 13 systemically and periodontally healthy subjects. The expression of mCD14 was determined by flow cytometry and expressed as mean intensity of fluorescence (MIF). Serum sCD14 level was examined with ELISA method. The expressions of mCD14 on monocytes in GAP patients and control subjects were comparable. No statistically significant differences were noted in the mean serum sCD14 level between GAP and control subjects. As periodontitis is a local disorder affecting a small fragment of the oral cavity it seems likely that chronic bacterial infection existing there is not reflected in the peripheral parameters.
    Advances in Medical Sciences 02/2006; 51 Suppl 1:166-9. · 0.96 Impact Factor
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    ABSTRACT: Aggressive forms of periodontitis lead to rapid bone destruction resulting in extensive losses in children's and young adults' dentition. Adhesion molecule deficiency syndrome and abnormalities in the expression of various adhesion molecules on peripheral blood leukocytes can be observed in prepubertal and aggressive periodontitis (AP) patients. The aim of the study was thus to assess the expression of selected cell adhesion molecules (CAMs; CD11a, CD11b, CD11c, CD54, and CD62L) on monocytes, neutrophils, and lymphocytes of the peripheral blood in patients with AP. The study involved 16 patients with AP and a control group of 13 generally healthy subjects with healthy periodontium. CAM expressions were determined by flow cytometry and presented as mean fluorescence intensity (MFI) and percentage of cells showing expression of the assessed adhesion molecules. Neutrophil CAM expressions in AP patients were comparable with those of the control group. MFI of CD62L on monocytes in AP patients was significantly lower than that of the controls. Lymphocytes showed increased CD11b expression compared with the control group. The percentage of leukocytes showing CAM expression in both groups was similar. Only the percentage of lymphocytes with CD11b in AP patients was significantly higher than in healthy controls. Because of the evident lack of differences between patients and controls and the great amount of individual dispersion of the results, the above CAMs on peripheral blood leukocytes in generally healthy patients with AP do not seem to be characteristic markers of this disease.
    Archivum Immunologiae et Therapiae Experimentalis 53(3):266-71. · 2.82 Impact Factor