J Phromjai

Publications (3)8.42 Total impact

• Article: In vitro cultivation of shrimp Taura syndrome virus (TSV) in a C6/36 mosquito cell line.

  N Arunrut, J Phromjai, W Gangnonngiw, N Kanthong, S Sriurairatana, W Kiatpathomchai
  Journal of Fish Diseases 08/2011; 34(10):805-10. · 2.00 Impact Factor
• Article: Association analysis of susceptibility candidate region on chromosome 5q31 for tuberculosis.

  C Ridruechai, S Mahasirimongkol, J Phromjai, H Yanai, N Nishida, I Matsushita, J Ohashi, N Yamada, S Moolphate, S Summanapan, C Chuchottaworn, W Manosuthi, P Kantipong, S Kanitvittaya, P Sawanpanyalert, N Keicho, S Khusmith, K Tokunaga
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  ABSTRACT: Chromosome 5q31 spans the T helper (Th) 2-related cytokine gene cluster, which is potentially important in Th1/Th2 immune responses. The chromosome 5q23.2-31.3 has been recently identified as a region with suggestive evidence of linkage to tuberculosis in the Asian population. With the aim of fine-mapping a putative tuberculosis susceptibility locus, we investigated a family-based association test between the dense single nucleotide polymorphism (SNP) markers within chromosome 5q31 and tuberculosis in 205 Thai trio families. Of these, 75 SNPs located within candidate genes covering SLC22A4, SLC22A5, IRF1, IL5, RAD50, IL13, IL4, KIF3A and SEPT8 were genotyped using the DigiTag2 assay. Association analysis revealed the most significant association with tuberculosis in haplotypes comprising SNPs rs274559, rs274554 and rs274553 of SLC22A5 gene (P(Global)=2.02 x 10(-6)), which remained significant after multiple testing correction. In addition, two haplotypes within the SLC22A4 and KIF3A region were associated with tuberculosis. Haplotypes of SLC22A5 were significantly associated with the expression levels of RAD50 and IL13. The results show that the variants carried by the haplotypes of SLC22A4, SLC22A5 and KIF3A region potentially contribute to tuberculosis susceptibility among the Thai population.
  Genes and immunity 07/2010; 11(5):416-22. · 4.22 Impact Factor
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  Available from: int-res.com

  Article: Different reactions obtained using the same DNA detection reagents for Thai and Korean hepatopancreatic parvovirus of penaeid shrimp.

  J Phromjai, W Sukhumsirichart, C Pantoja, D V Lightner, T W Flegel
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  ABSTRACT: Hepatopancreatic parvovirus (HPV) can cause stunted growth and death in penaeid shrimp including Penaeus monodon. We used PCR primers and a commercial DNA probe designed from HPV of Penaeus chinensis (HPVchin) to examine HPV-infected Thai P. monodon (HPVmon). We found that the PCR primers produced a 732 bp DNA amplicon rather than the 350 bp amplicon obtained with HPVchin template and that the DNA probe gave weak to variable in situ DNA hybridization results. In addition, hybridization to PCR products from HPVmon was weak compared with hybridization with PCR products from HPVchin. By contrast, the 732 bp amplicon hybridized strongly with HPVmon-infected cells by in situ hybridization but not with uninfected shrimp tissue or other shrimp viruses, thus confirming its origin from HPVmon. Cloning, sequencing and analysis of the 732 bp amplicon showed that 696 bp (excluding the primer sequences) contained 47% GC content and had only 78% homology to 701 aligned bases from a 3350 bp DNA fragment of HPVchin from GenBank. These results explain why the reagents based on HPVchin gave a different PCR product and weak hybridization results with HPVmon, and they show that multiple primers or degenerate primers may be necessary for general detection of HPV varieties. Together with previously published information on the estimated total genome sizes for HPVchin (approximately 4 kb) and HPVmon (approximately 6 kb), these data support the contention that HPVchin and HPVmon are different varieties or species, in spite of their similar histopathology.
  Diseases of Aquatic Organisms 10/2001; 46(2):153-8. · 2.20 Impact Factor