Publications (5)2.4 Total impact
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Article: Comparison of the DNA Fragmentation and the Sperm Parameters after Processing by the Density Gradient and the Swim up Methods.
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ABSTRACT: The swim up and the density-gradient centrifugation are the two main techniques which are used to separate the viable motile sperm fraction in the assisted reproductive technology. However, there are several published studies about these methods, but there is no sufficient evidence for recommending the superiority of one of them. This study was designed to study the efficiency of the swim-up and the density gradient techniques to recover the spermatozoa with a high degree of motility, a normal morphology and a low level of DNA fragmentation. A total of 35 semen samples were included in the study. The semen samples were collected, one part of the semen was spread on a slide and the remainder was prepared by using the swim-up or the density gradient techniques. The recovered spermatozoa were evaluated for concentration, motility, and normal morphology. A comet assay was carried out to assay the DNA fragmentation in all the samples. There were significant differences in the sperm parameters between the density gradient and the swim up techniques. Also, the swim-up technique showed a significantly higher level of DNA fragmentation as compare to the density gradient technique. The results of this study demonstrated several benefits of the gradients method in the separation of normal and motile spermatozoa with healthy DNA, in comparison to the swim up method.Journal of clinical and diagnostic research : JCDR. 11/2012; 6(9):1451-3. -
Article: Apolipoprotein E genotypes of fertile and infertile men.
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ABSTRACT: Lipid components of spermatozoa have an important role in the functional activity of this cell. The protein, apolipoprotein E (apoE), has a central role in lipid transport. The aim of this study was to investigate the distribution of APOE genotypes, ϵ3ϵ3, ϵ3ϵ4, and ϵ2ϵ3, and the corresponding alleles in fertile and infertile males, and to assess the semen parameters from the patients carrying the different alleles. In addition, the levels of cholesterol, phospholipid, and triacylglycerol in spermatozoa, isolated by PureSperm gradient and from seminal plasma in samples from infertile males was compared with respect to the APOE genotype. APOE genotypes were determined by PCR-RFLP on DNA extracted from peripheral blood leucocytes in 108 fertile and 107 infertile men. There was a significant difference between the distribution of APOE genotypes in fertile as compared to infertile males (χ(2) = 9.1, df = 2, p = 0.011). The presence of genotype ϵ3ϵ4 conferred a 3.82 risk factor for male infertility {Odds ratio = 3.82 (1.46-10, p = 0.006)}. Our findings showed that the distribution of APOE genotypes and alleles differed between fertile and infertile individuals and may be a risk factor for male infertility. We suggest that the effects of APOE genotypes may be linked to differences in the efficacy of the expressed apoE isoforms in promoting sperm maturation during epididymal transit.Systems biology in reproductive medicine 05/2012; 58(5):263-7. · 0.80 Impact Factor -
Article: Association between nitric oxide and 8-hydroxydeoxyguanosine levels in semen of diabetic men
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ABSTRACT: The incidence of diabetes mellitus is rapidly increasing in the world. One of the complications of diabetes includes disturbance of the reproductive tract, such as infertility, erectile dysfunction, and endocrine disruption. Nitric oxide (NO) is a free radical produced by most cells including the human male and female reproductive tracts. NO has a dual role where low concentrations are essential for homeostatic cellular biology and physiology, but high levels have detrimental effects relating to cellular damage from this reactive oxygen species (ROS). 8-hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside of DNA that is currently used as a biomarker of cellular oxidative stress, where urinary levels can correlate with diabetic nephropathy and retinopathy. Our aim was to investigate the relationship between nitrate/nitrite levels and 8-OHdG levels in the semen of diabetic and non-diabetic men. Concentrations of nitrate/nitrite and 8-OHdG were examined in seminal plasma of 32 diabetic and 35 non-diabetic men. The level of nitrate/nitrite was assayed by colorimetric reaction and 8-OHdG was measured by ELISA. Our results showed that the seminal plasma nitrate/nitrite levels were significantly higher in the diabetic group (p < 0. 01). There were also significantly higher 8-OHdG levels in diabetic men compared to non-diabetic men (p < 0.05). Regression analysis indicated that in diabetic men, nitrate/nitrite levels correlated well with 8-OHdG levels (r = 0.64, p < 0.001). A significant trend between nitrate/nitrite and sperm parameters was not observed. Our data suggests that high levels of nitrate/nitrite in the semen of diabetic men is suggestive of reactive oxygen species induced DNA damage that is correlated with 8-OHdG levels but not sperm parameters. These results support the further investigation of NO and 8-OHdG as biomarkers for assessing male infertility.11/2011; 57(6):292-295. -
Article: Association between nitric oxide and 8-hydroxydeoxyguanosine levels in semen of diabetic men.
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ABSTRACT: The incidence of diabetes mellitus is rapidly increasing in the world. One of the complications of diabetes includes disturbance of the reproductive tract, such as infertility, erectile dysfunction, and endocrine disruption. Nitric oxide (NO) is a free radical produced by most cells including the human male and female reproductive tracts. NO has a dual role where low concentrations are essential for homeostatic cellular biology and physiology, but high levels have detrimental effects relating to cellular damage from this reactive oxygen species (ROS). 8-hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside of DNA that is currently used as a biomarker of cellular oxidative stress, where urinary levels can correlate with diabetic nephropathy and retinopathy. Our aim was to investigate the relationship between nitrate/nitrite levels and 8-OHdG levels in the semen of diabetic and non-diabetic men. Concentrations of nitrate/nitrite and 8-OHdG were examined in seminal plasma of 32 diabetic and 35 non-diabetic men. The level of nitrate/nitrite was assayed by colorimetric reaction and 8-OHdG was measured by ELISA. Our results showed that the seminal plasma nitrate/nitrite levels were significantly higher in the diabetic group (p < 0. 01). There were also significantly higher 8-OHdG levels in diabetic men compared to non-diabetic men (p < 0.05). Regression analysis indicated that in diabetic men, nitrate/nitrite levels correlated well with 8-OHdG levels (r = 0.64, p < 0.001). A significant trend between nitrate/nitrite and sperm parameters was not observed. Our data suggests that high levels of nitrate/nitrite in the semen of diabetic men is suggestive of reactive oxygen species induced DNA damage that is correlated with 8-OHdG levels but not sperm parameters. These results support the further investigation of NO and 8-OHdG as biomarkers for assessing male infertility.Systems biology in reproductive medicine 11/2011; 57(6):292-5. · 0.80 Impact Factor -
Article: Genetic variation of hormone sensitive lipase and male infertility.
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ABSTRACT: Hormone sensitive lipase (HSL) is a triacylglycerol hydrolase and cholesterol esterase that is essential for male fertility. The aim of the study was to investigate the distribution of C-60G polymorphism of HSL gene and alleles in fertile and infertile males living in Hamadan, Iran. In addition, lipase activity was determined in these two groups. The HSL genotype was determined by PCR-RFLP and the lipase activity was detected by turbidometery in 164 fertile and 169 infertile males. A significant difference in HSL genotype distribution was observed between groups (χ2 = 8.1, df = 2, p = 0.017). Infertile males showed a higher percentage of CC as well as a lower percentage of CG and GG genotype compared with fertile individuals. The presence of CC to CG + GG genotype conferred a 2.4-fold risk for male infertility (odds ratio = 2.4 (1.3 - 4.5), p = 0.006). In addition, lipase activity was remarkably higher (p < 0.001) in fertile males (94 ± 66 U/L) compared to the infertile subjects (50.6 ± 49 U/L). This suggests that genetic variation of HSL may be a risk factor for male infertility.Systems biology in reproductive medicine 09/2011; 57(6):288-91. · 0.80 Impact Factor
