Publications (2)7.12 Total impact
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Article: Resveratrol derivative-rich melinjo (Gnetum gnemon L.) seed extract suppresses multiple angiogenesis-related endothelial cell functions and tumor angiogenesis.
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ABSTRACT: Angiogenesis is a promising target for cancer prevention and treatment. This study aimed to determine the antiangiogenic effects of melinjo (Gnetum gnemon L.) seed extract and its resveratrol derivative components, such as gnetin C (GC), gnetin L (GL), gnemonoside A (GMA), gnemonoside C (GMC), and gnemonoside D (GMD). An ethanol extract of melinjo seeds (EEMS) and the two gnetins markedly inhibited the proliferation and tube formation of human umbilical vein endothelial cells (HUVEC) stimulated with vascular endothelial growth factor and basic fibroblast growth factor. The inhibitory effects of GC and GL were much stronger than those of resveratrol. GMC and GMD inhibited only proliferation, whereas GMA had almost no effect on the two endothelial cell functions. The EEMS and GC also reduced the cell viability of tube-forming HUVEC, with accompanying ERK1/2 inactivation, and suppressed the migration of HUVEC. Furthermore, dietary intake of EEMS significantly inhibited tumor angiogenesis in a mouse dorsal air sac assay. In conclusion, we found that the EEMS and its resveratrol derivatives, particularly GC, suppress multiple angiogenesis-related endothelial cell functions and/or tumor angiogenesis, indicating that the melinjo seeds and the natural resveratrol derivatives may be useful for cancer prevention and treatment.Molecular Nutrition & Food Research 09/2011; 55(11):1730-4. · 4.30 Impact Factor -
Article: Stilbenoids isolated from the seeds of Melinjo (Gnetum gnemon L.) and their biological activity.
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ABSTRACT: A 50% EtOH extract of the dried endosperms of melinjo (Gnetum gnemon L.) was purified by a combination of column chromatography, including highly porous polymer, octadecylated silica (ODS), hydroxypropylated dextran (LH-20), and silica gel, resulting in the isolation of a new stilbenoid 1, named gnetin L, along with five previously identified stilbenoids 2-6 (gnetin C, gnemonosides A, C, and D, and resveratrol). All of these stilbenoids showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity similar to that of ascorbic acid and dl-alpha-tocopherol. With the exception of gnemonoside A (3), these stilbenoids showed moderate antimicrobial activity. Inhibition of lipase from porcine pancreas was recognized in four stilbenoids excluding gnemonoside A (3) and resveratrol (6). Gnetin C (2), gnemonoside C (4), and gnemonoside D (5) inhibited the hydrolysis of starch by alpha-amylase from porcine pancreas. An EtOH extract containing these stilbenoids also showed DPPH radical scavenging effect, lipase and alpha-amylase inhibition activity, and antimicrobial activity against food microorganisms and enterobacteria. The present study indicates that melinjo and extracts containing these constituents are useful as health supplements.Journal of Agricultural and Food Chemistry 03/2009; 57(6):2544-9. · 2.82 Impact Factor
