[Show abstract][Hide abstract] ABSTRACT: Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this sporogenic lactic acid bacterium to grow at 50-55 °C and pH 5.0 makes this organism an attractive microbial biocatalyst for production of optically pure lactic acid at industrial scale not only from glucose derived from cellulose but also from xylose, a major constituent of hemicellulose. This bacterium is also considered as a potential probiotic. Complete genome sequence of a representative strain, B. coagulans strain 36D1, is presented and discussed.
Standards in Genomic Sciences 12/2011; 5(3):331-40. · 3.17 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Thermotoga sp. strain RQ2 is probably a strain of Thermotoga maritima. Its complete genome sequence allows for an examination of the extent and consequences of gene flow within Thermotoga species and strains. Thermotoga sp. RQ2 differs from T. maritima in its genes involved in myo-inositol metabolism. Its genome also encodes an apparent fructose phosphotransferase system (PTS) sugar transporter. This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Thermotogales. These are the first reported PTS transporters in the Thermotogales.
Journal of bacteriology 10/2011; 193(20):5869-70. · 2.69 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Kosmotoga olearia strain TBF 19.5.1 is a member of the Thermotogales that grows best at 65°C and very well even at 37°C. Information about this organism is important for understanding the evolution of mesophiles from thermophiles. Its genome sequence reveals extensive gene gains and a large content of mobile genetic elements. It also contains putative hydrogenase genes that have no homologs in the other member of the Thermotogales.
Journal of bacteriology 10/2011; 193(19):5566-7. · 2.69 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Background / Purpose:
Zymomonas mobilis is an alpha-proteobacterium that converts sugars into ethanol several-fold faster than yeasts, with simpler requirements and yielding minimal biomass. Additionally, its genome is only 2-Mb large and thus more inviting to genetic engineering manipulations. For these reasons, large industries as well as research and academic laboratories currently invest in the study and improvement of this organism.Z. mobilis has been explored for years at the University of Athens, Greece (studies on plasmids, stress, mutagenesis, genetic tool development etc). At present, two on-going projects with the US DOE-JGI investigate Z. mobilis genomics and transcriptomics. Six different strains belonging to two of its major subspecies (mobilis and pomaceae), isolated from various geographic locations, were chosen to be examined in a comparative genomics context.
The organisms sequenced, despite belonging to the same species, bear differences in gene content and order in both their chromosomal and extrachromosomal (plasmid) genomic component. This variability suggests that the pangenome of the species maybe larger than thought and provides basis for functional genomics prospects.
Hellenic Society for Computational Biology and Bioinformatics 2010 Conference; 12/2010