Aline M C Ramos-Fregonezi

Universidade Federal do Rio Grande do Sul, Porto Alegre, Estado do Rio Grande do Sul, Brazil

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Publications (3)7.76 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The garden petunia is derived from Petunia axillaris (white flowered) and a purple-flowered species in the P. integrifolia group; it is still unclear which purple-flowered species was used in the initial crosses. This widely cultivated hybrid is an ideal model for different areas of scientific inquiry. We analyzed three taxa of the P. integrifolia group considered to be the most probable parental candidates, along with a random sample of garden petunia representatives that were selected for their genetic variability. We used cpDNA trnH-psbA and trnS-trnG haplotypes and seven nuclear microsatellites in a population approach to investigate the genetic variability. This is the first time information from plastid DNA sequences and nuclear microsatellites has been combined to infer evolutionary relationships in these taxa. Our results suggest that P. interior is the purple ancestor of garden petunias, and we postulate that the initial crosses must have been between both parents as a mother plant. Our work will contribute to the clarification of the evolutionary relationships among the Petunia ×hybrida and P. integrifolia taxa group and could be useful in breeding programs to transfer desired traits from wild to cultivated species.
    American Journal of Botany 12/2013; · 2.59 Impact Factor
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    ABSTRACT: • Premise of the study: Microsatellite primers were developed in Aureliana fasciculata var. fasciculata, a shrub that inhabits the Atlantic Rainforest, to investigate the intraspecific genetic structure of the species. • Methods and Results: Nine pairs of microsatellite primers were designed that were based on a simple sequence repeat (SSR)-enriched library. Seven loci were amplified successfully. Two populations of A. fasciculata var. fasciculata were genotyped and all loci were polymorphic, with two to five alleles per locus per population detected. Cross-species amplification was achieved in other members of the genus and members of the sister genus Athenaea. • Conclusions: The development of these microsatellite markers will contribute to future population genetic studies in A. fasciculata var. fasciculata. Cross-amplification among other species in the genus and in the sister genus Athenaea might be a useful tool to establish patterns of genetic variability within the Atlantic Rainforest.
    American Journal of Botany 03/2012; 99(4):e173-5. · 2.59 Impact Factor
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    ABSTRACT: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.
    American Journal of Botany 09/2011; 98(10):e277-9. · 2.59 Impact Factor

Publication Stats

7.76 Total Impact Points

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Institutions

  • 2011–2012
    • Universidade Federal do Rio Grande do Sul
      • Departamento de Genética
      Porto Alegre, Estado do Rio Grande do Sul, Brazil