Adam Fraser

Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States

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Publications (4)37.21 Total impact

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    ABSTRACT: Experimental reproducibility is fundamental to the progress of science. Irreproducible research decreases the efficiency of basic biological research and drug discovery and impedes experimental data reuse. A major contributing factor to irreproducibility is difficulty in interpreting complex experimental methodologies and designs from written text and in assessing variations among different experiments. Current bioinformatics initiatives either are focused on computational research reproducibility (i.e. data analysis) or laboratory information management systems (LIMS). Here we present a software tool, ProtocolNavigator, which addresses the largely-overlooked challenges of interpretation and assessment. It provides a biologist-friendly, open-source, emulation based tool for designing, documenting, and reproducing biological experiments. Availability and implementation: ProtocolNavigator was implemented in Python 2.7, using the wx module to build the graphical user interface. It is a platform independent software and freely available from http://protocolnavigator.org/index.html under the GPL v2 license.
    Bioinformatics 08/2014; DOI:10.1093/bioinformatics/btu554 · 4.62 Impact Factor
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    ABSTRACT: Automated microscopes have enabled the unprecedented collection of images at a rate that precludes visual inspection. Automated image analysis is required to identify interesting samples and extract quantitative information for high-content screening (HCS). However, researchers are impeded by the lack of metrics and software tools to identify image-based aberrations that pollute data, limiting experiment quality. The authors have developed and validated approaches to identify those image acquisition artifacts that prevent optimal extraction of knowledge from high-content microscopy experiments. They have implemented these as a versatile, open-source toolbox of algorithms and metrics readily usable by biologists to improve data quality in a wide variety of biological experiments.
    Journal of Biomolecular Screening 09/2011; 17(2):266-74. DOI:10.1177/1087057111420292 · 2.01 Impact Factor
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    ABSTRACT: There is a strong and growing need in the biology research community for accurate, automated image analysis. Here, we describe CellProfiler 2.0, which has been engineered to meet the needs of its growing user base. It is more robust and user friendly, with new algorithms and features to facilitate high-throughput work. ImageJ plugins can now be run within a CellProfiler pipeline. AVAILABILITY AND IMPLEMENTATION: CellProfiler 2.0 is free and open source, available at http://www.cellprofiler.org under the GPL v. 2 license. It is available as a packaged application for Macintosh OS X and Microsoft Windows and can be compiled for Linux. CONTACT: anne@broadinstitute.org SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
    Bioinformatics 02/2011; 27(8):1179-80. DOI:10.1093/bioinformatics/btr095 · 4.62 Impact Factor
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    ABSTRACT: Advances in imaging techniques and high-throughput technologies are providing scientists with unprecedented possibilities to visualize internal structures of cells, organs and organisms and to collect systematic image data characterizing genes and proteins on a large scale. To make the best use of these increasingly complex and large image data resources, the scientific community must be provided with methods to query, analyze and crosslink these resources to give an intuitive visual representation of the data. This review gives an overview of existing methods and tools for this purpose and highlights some of their limitations and challenges.
    Nature Methods 03/2010; 7(3 Suppl):S26-41. DOI:10.1038/nmeth.1431 · 25.95 Impact Factor