Yu Kakimoto

Publications (5)9.04 Total impact

• Source

  Available from: Tatsuaki Tsuruyama

  Dataset: journal.pone.0041607

  Yu Kakimoto, Tatsuaki Tsuruyama, Takushi Yamamoto, Masaru Furuta, Hirokazu Kotani, Munetaka Ozeki, Akihiko Yoshizawa, Hironori Haga, Keiji Tamaki
• Source

  Available from: Tatsuaki Tsuruyama

  Article: Novel In Situ Pretreatment Method for Significantly Enhancing the Signal In MALDI-TOF MS of Formalin-Fixed Paraffin-Embedded Tissue Sections

  Yu Kakimoto, Tatsuaki Tsuruyama, Takushi Yamamoto, Masaru Furuta, Hirokazu Kotani, Munetaka Ozeki, Akihiko Yoshizawa, Hironori Haga, Keiji Tamaki
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  ABSTRACT: The application of matrix-assisted laser desorption/ionization (MALDI)-based mass spectrometry (MS) to the proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue presents significant technical challenges. In situ enzymatic digestion is frequently used to unlock formalin-fixed tissues for analysis, but the results are often unsatisfactory. Here, we report a new, simplified in situ pretreatment method for preparing tissue sections for MS that involves heating with vapor containing acetonitrile in a small airtight pressurized space. The utility of the novel method is shown using FFPE tissue of human colon carcinoma. The number and intensity of MALDI peaks obtained from analysis of pretreated tissue was significantly higher than control tissue not subjected to pretreatment. A prominent peak (m/z 850) apparently specific to cancerous tissue was identified as a fragment of histone H2A in FFPE tissue pretreated using our method. This highly sensitive treatment may enable MALDI-MS analysis of archived pathological FFPE samples, thus leading to the identification of new biomarkers.
  PLoS ONE 08/2012; 7(8):e41607. · 4.09 Impact Factor
• Article: Pituitary abscess presenting a very rapid progression: report of a fatal case.

  Hirokazu Kotani, Hitoshi Abiru, Masashi Miyao, Yu Kakimoto, Chihiro Kawai, Munetaka Ozeki, Tatsuaki Tsuruyama, Keiji Tamaki
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  ABSTRACT: Pituitary abscess is a rare disease presenting with nonspecific clinical symptoms, and diagnosis is often difficult. This disease is potentially life-threatening, but most cases have a chronic and indolent course. We report a case of a 60-year-old man with a pituitary abscess associated with pituitary adenoma who died 5 days after the onset of clinical symptoms without a definitive diagnosis. Postmortem computed tomography and autopsy findings revealed a sellar mass with cystic change and extension toward the optic chiasm. Histopathology of the lesion demonstrated an abscess with suppurative meningitis and encephalitis. The disturbance of the cardiac autonomic nervous system because of hypothalamus involvement was suggested as the cause of rapid progression and death. This case provides useful information for clinicians to avoid a lethal outcome.
  The American journal of forensic medicine and pathology: official publication of the National Association of Medical Examiners 07/2012; 33(3):280-3. · 0.71 Impact Factor
• Article: Transmesenteric hernia due to double-loop formation in the small intestine: a fatal case involving a toddler.

  Yu Kakimoto, Hitoshi Abiru, Hirokazu Kotani, Munetaka Ozeki, Tatsuaki Tsuruyama, Keiji Tamaki
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  ABSTRACT: We report a unique case of transmesenteric hernia resulting in death, which went undiagnosed during a recent hospital visit. The victim was a 2.5-year-old girl who - with the exception of chronic constipation - had no medical history. One night she complained of abdominal pains and was taken to a pediatric hospital where doctors performed an abdominal X-ray and echography. No significant findings suggesting bowel obstruction (e.g. air-fluid levels or dilation of the bowel) were obtained on examinations and bloody feces were not observed in this particular episode. As her abdominal pain gradually attenuated, the doctor allowed her to return home. A few hours later, she lost consciousness and expired despite resuscitation efforts attempted at an emergency hospital. A subsequent autopsy revealed that the small bowel had herniated through a defect in the mesentery resulting in two consecutive and inversely forming loops, in which each loop protruded on either side of the mesentery. This rare morphological anatomy seems to have progressed in a two-step process. The girl's mild abdominal pain was likely induced by herniation and formation of the first intestinal loop, followed by severe shock occurring when the subsequent intestinal segment invaginated into the same defect forming the second loop on the opposite side of the mesentery. This case illustrates the difficulty of diagnosing transmesenteric hernia due to the presentation of unspecific symptoms; especially in infants and toddlers. Furthermore, this report demonstrates the value of a complete autopsy in cases of sudden and unexpected deaths involving children.
  Forensic science international 08/2011; 214(1-3):e39-42. · 2.10 Impact Factor
• Article: Intrasubject reproducibility of prefrontal cortex activities during a verbal fluency task over two repeated sessions using multi-channel near-infrared spectroscopy.

  Yu Kakimoto, Yukika Nishimura, Naomi Hara, Motohiro Okada, Hisashi Tanii, Yuji Okazaki
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  ABSTRACT: To determine whether intrasubject reproducibility could be observed in the frontal cortex and to assess the mental-health status of subjects in each session. We measured changes in oxygenated hemoglobin concentration ([oxy-Hb]) during a letter version of the verbal fluency task using near-infrared spectroscopy imaging in twenty healthy adults over two sessions approximately two months apart. Additionally, the mental-health status of the subjects in each session was evaluated according to the State-Trait Anxiety Inventory, the Zung Self-rating Depression Scale, the Profile of Mood States, and the revised edition of the Neuroticism-Extroversion-Openness Personality Inventory. The association between those scores and [oxy-Hb] changes during the verbal fluency task in each session was investigated. Performance on the verbal fluency task was about equal across the two sessions, and frontal activation during the task was observed globally in approximately the same region. In the test-retest reliability, acceptable values were shown in both the Intraclass Correlation Coefficients of the mean [oxy-Hb] changes and the correlation coefficients of the whole waveforms for each subject in the two sessions. Mental-health status as measured by several questionnaires was within the healthy range, and no correlation with the frontal activation was seen, except in several channels. The current results suggest that the measurement experience exerted very little influence, except for in a very small region. In addition, the intrasubject reproducibility of frontal activation measured by multi-channel near-infrared spectroscopy was well demonstrated in mentally healthy subjects at intervals of two months.
  Psychiatry and Clinical Neurosciences 06/2009; 63(4):491-9. · 2.13 Impact Factor