Yong Zhao

Publications (2)2.34 Total impact

• Article: Expression of apoptosis-related genes in the endometrium of polycystic ovary syndrome patients during the window of implantation.

  Ling Yan, Aiming Wang, Lei Chen, Wei Shang, Min Li, Yong Zhao
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  ABSTRACT: The present study investigated the expression of the apoptosis-related genes fas-associated via death domain (FADD) and Bcl-2 in the endometrium during the window of implantation in polycystic ovary syndrome (PCOS) patients. The aim was to explore the role of cell apoptosis in endometrial receptivity during this period. The subjects were divided into experimental and control group. The experimental group comprised 12 infertile women with PCOS, and the control group comprised 12 women who were infertile because of tubal pathological factors but had normal menstrual cycles. Endometria were collected by biopsy 7d after ovulation. Six samples from each group were randomly selected and subjected to gene chip analyses. The expression of endometrial FADD and Bcl-2 was determined by immunohistochemistry, and cell apoptosis was detected by the TUNEL method. Compared with the control group, 194 differentially expressed genes were found in the PCOS group, 102 of which were upregulated and 92 were downregulated. The differentially expressed genes were divided into 15 types according to function. Among the nine genes related to cell apoptosis, five (including Bcl-2) were upregulated and four were downregulated (including FADD). Bcl-2 expression during the window of implantation in the PCOS group increased compared with the control group, showing a significant difference (P<0.05). FADD expression in the PCOS group notably decreased compared with that in the control group, which also showed a significant difference (P<0.05). Cell apoptosis analysis showed a significant difference between the average apoptotic indices in the PCOS and control groups (P<0.05). Significant differences were observed between the endometrial gene expression in the PCOS and control groups. The decrease in cell apoptosis during the window of implantation in PCOS patients may be one of the causes of the reduced endometrial receptivity.
  Gene 07/2012; 506(2):350-4. · 2.34 Impact Factor
• Article: [Sperm DNA damage and unexplained recurrent spontaneous abortion].

  Cheng-Jun Liu, Ai-Ming Wang, Wei Shang, Min Li, Wei-Zhou Wang, Yu-Liang Shen, Yong Zhao
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  ABSTRACT: To explore the relationship of sperm DNA damage with unexplained recurrent spontaneous abortion (URSA). Sperm DNA fragmentation was evaluated by sperm chromatin dispersion (SCD), and the results were expressed in terms of DNA fragmentation index (DFI). DFIs were measured in the male partners of 56 women with URSA (the experiment group) and of 31 without URSA (the control group). The DFI was shown to be (11.0% - 56.9%) in the experiment group, 21 (37.5%) of the subjects over 30%, as compared with (10.0% -36.8%) in the control group, only 8 (25.8%) of the subjects over 30%, significantly higher in the former than in the latter (29.4% vs 25.5%, P < 0.05). There is a relationship between sperm DNA damage and URSA.
  Zhonghua nan ke xue = National journal of andrology 07/2011; 17(7):619-21.