Xiaohua Wang

Tianjin University of Science and Technology, T’ien-ching-shih, Tianjin Shi, China

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Publications (7)12.79 Total impact

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    ABSTRACT: The salt-tolerant Torulopsis versatilis mutant strain, T3-5, is able to grow in YPD medium containing 22% salt, whereas the original strain, T, used for soy sauce fermentation was only able to grow in the presence of 20% or less. Biochemical analyses showed that strain T3-5 had a 22% higher intracellular glycerol level than T. Sequencing of the glycerol transporter gene FPS1 revealed a change from G to A at position 198 upstream of the start codon, changes from G to A at positions 93 and 1084, respectively, within the FPS1 gene, and insertions of T base at positions 285 and 310, respectively, downstream of the termination codon when comparing the corresponding sequences of strains T and T3-5. An inverse relationship between osmotic pressure and the FPS1 transcription level was observed for both T and T3-5 using qPCR. Therefore, it is possible that lower transcription levels of FPS1 are at least partly responsible for increased osmotolerance in strain T3-5. Overexpression of T and T3-5 FPS1 in Saccharomyces cerevisiae resulted in lower salt tolerance, especially in the presence of 12% salt. The data suggest that decreased expression levels of and/or alteration in the FPS1 protein contributed to increased salt tolerance of T3-5.
    International Journal of Food Science & Technology 03/2014; 49(3). · 1.24 Impact Factor
  • Baojiao Gao, Xiaohua Wang, Yanyan Zhang
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    ABSTRACT: An anion-imprinted material with high performance for recognizing chromate anion was prepared by adopting the novel molecule surface-imprinting technique put forward by our research group previously. Functional macromolecule polyvinylimidazole (PVI) was first grafted on the surfaces of micron-sized silica gel particles, forming the grafted particle PVI/SiO2. Afterwards, the ion imprinting was carried out towards the grafted macromolecule PVI by using 4CrO2−CrO42− anion as template and 1,6-dibromohexane as crosslinking agent, and chromate ion surface-imprinted material IIP-PVI/SiO2 was obtained. The binding characteristic of IIP-PVI/SiO2 towards 4CrO2−CrO42− anion was studied with both static and dynamic methods. The experimental results show that IIP-PVI/SiO2 has excellent binding affinity and high recognition selectivity for chromate anion. Before imprinting, the grafted particles PVI/SiO2 have similar adsorption capacities for both two anions, chromate anion and phosphate anion that was used as a contrast anion. However, after imprinting, the binding capacity of IIP-PVI/SiO2 towards phosphate anion decreases remarkably, whereas the binding capacity of IIP-PVI/SiO2 for chromate anion still remains very high, leading to a high selectivity coefficient (7.78) of IIP-PVI/SiO2 for 4CrO2−CrO42− anion with respect of 4PO3−PO43− ion.
    Materials Chemistry and Physics. 07/2013; 140(s 2–3):478–486.
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    ABSTRACT: Soy sauce is considered the most important food condiment in China for its distinct fragrance and salty taste. Highly valued for its unique tastes and flavours, soy sauce exhibits many beneficial functions, such as 4‐hydroxy‐2(or 5)‐ethyl‐5(or 2)‐methyl‐3(2H)‐furanone and 4‐ethylguaiacol. Soy sauce has been widely used in Asian countries for cooking. With the rapidly growing popularity of these unique tastes and flavours, it is important to have a comprehensive reference of its functional benefits. Meanwhile, the genes encoding the major enzymes of metabolism exhibit specialised roles. This review provides a valuable source for the abundant metabolism genes of strains and current knowledge on antioxidant activity, antihypertensive effect, anticarcinogenic activity, fortification and their relationships with soy sauce tastes and flavours.
    International Journal of Food Science & Technology 01/2013; 48(5). · 1.24 Impact Factor
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    ABSTRACT: Trehalose can protect organisms against disadvantage conditions, such as high osmolarity, heat, oxidation, desiccation and freezing. In this work, the ability to produce trehalose of soy sauce yeasts was studied first and the results showed that trehalose could be accumulated gradually in logarithmic phase and achieve a concentration peak in stationary phase. Then the relevance of growth conditions and intracellular trehalose level of soy sauce yeasts was investigated. Mutant strains (S3-2, T3-5) which can grow in hypertonic conditions, showed different results from their original organisms Zygosaccharomyces rouxii (S) and Torulopsis versatilis (T). For S and T could increase trehalose level from 7.0% and 7.5% to 8.4% and 9.6% with 9% salt stimulation, respectively. While the mutants could accumulate more trehalose under a higher salt (about 12%) stimulation compared to 0% NaCl situation, which suggested a significant feature of the mutants. And the original strains could increase the trehalose level from 8.4% and 9.6% to 10.1% and 10.9% respectively under heat shock (40 °C, 30mins). Moreover, the mutant strains could also product more trehalose in the thermal stimulation condition, but less than the original ones.
    IERI Procedia. 01/2013; 5:321–326.
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    ABSTRACT: In this study, 5-aminosalicylic acid (5-ASA) was successfully grafted onto the poly(glycidyl methacrylate) (PGMA) macromolecular chains of PGMA/SiO(2) to obtain adsorbent ASA-PGMA/SiO(2). The adsorption properties of ASA-PGMA/SiO(2) for phenolic compounds were studied through batch and column methods. The experimental results showed that ASA-PGMA/SiO(2) possesses strong adsorption ability for phenolic compounds, and its adsorption capacity for phenol, 4-chlorophenol, and p-nitrophenol reaches 1.0, 1.1, and 1.32 mmolg(-1), respectively. In addition, pH has a great influence on the adsorption capacity. The adsorption isotherm data obeyed the Langmuir model well than Freundlich model. The desorption of phenolic compounds from the ASA-PGMA/SiO(2) adsorbent was most effectively achieved in a 0.1 molL(-1) sodium hydroxide solution. Consecutive adsorption-desorption experiments showed that the ASA-PGMA/SiO(2) adsorbent could be reused almost without any loss in the adsorption capacity.
    Journal of hazardous materials 11/2011; 201-202:74-81. · 4.14 Impact Factor
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    ABSTRACT: In this study, 5-aminosalicylic acid was successfully grafted onto the poly(glycidyl methacrylate) (PGMA) macromolecular chains of PGMA/SiO(2) to obtain a novel adsorbent designated as ASA-PGMA/SiO(2). The adsorption properties of ASA-PGMA/SiO(2) for heavy metal ions were studied through batch and column methods. The experimental results showed that ASA-PGMA/SiO(2) possesses strong chelating adsorption ability for heavy metal ions, and its adsorption capacity for Cu(2+), Cd(2+), Zn(2+), and Pb(2+) reaches 0.42, 0.40, 0.35, and 0.31 mmol g(-1), respectively. In addition, pH has a great influence on the adsorption capacity in the studied pH range. The adsorption isotherm data greatly obey the Langmuir and Freundlich model. The desorption of metal ions from ASA-PGMA/SiO(2) is effective using 0.1 mol l(-1) of hydrochloric acid solution as eluent. Consecutive adsorption-desorption experiments showed that ASA-PGMA/SiO(2) could be reused almost without any loss in the adsorption capacity.
    Journal of hazardous materials 06/2011; 192(3):956-62. · 4.14 Impact Factor
  • Baojiao Gao, Li Fang, Xiaohua Wang, Jiying Men
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    ABSTRACT: In this work, a new and highly efficient method of surface-initiated free radical graft-polymerizations on the surfaces of silica gel particles was put forward, and the graft-polymerization of methacrylic acid (MAA) was conducted. This method was convenient, feasible and highly effective. Coupling agent γ-mercaptopropyltrimethoxysilane(MPTS) was first bonded onto the surfaces of silica gel particles, obtaining the modified particles MPTS-SiO2, onto which mercapto groups were chemically attached, so a redox initiation system of graft-polymerization was constituted by the mercapto group on the surfaces of MPTS-SiO2 particles and the cerium (IV) salt in the solution. And then the surface-initiated free radical graft-polymerization of MAA on the surfaces of silica gel particles was carried out, resulting in the grafted particles PMAA/SiO2 with a very high grafting density (35 g/100 g) of PMAA. The grafted particles PMAA/SiO2 were characterized by infrared spectrum (FTIR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA). The effects of the main factors on the new surface-initiated graft polymerization were emphatically examined, and the corresponding mechanism of the graft-polymerization was investigated in depth. The experimental results show that the mercapto group-cerium salt system analogous to the hydroxyl group-cerium salt system, can also effectively initiate vinyl monomers to be graft-polymerized on the surfaces of solid particles, and furthermore, it is a highly effective surface-initiated graft-polymerization method. In this graft-polymerization system, several factors such as sulfuric acid concentration, the used amount of cerium salt and the reaction temperature affect the grafting density greatly. For the graft-polymerization of MAA, the appropriate reaction conditions are as follows: reaction time of 3 h, reaction temperature of 50 °C, cerium concentration of 5.0 × 10−3 M, acid (H+ ion) concentration of 0.15 M and MAA concentration of 0.5 M.
    Journal of Polymer Research 19(11). · 2.02 Impact Factor