Xiaoli Chen

Publications (4)3.8 Total impact

• Article: A monoclonal antibody against the catalytic domain of PTP1B.

  Fanwei Zeng, Erru Ni, Shengyu Wang, Sha Lv, Chang Cao, Haipeng Zhang, Haiyan Lv, Xiaoli Chen, Jianghua Yan, Fanghong Luo
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  ABSTRACT: Protein tyrosine phosphatase 1B (PTP1B), a member of the protein tyrosine phosphatase (PTP) family, plays a crucial role in metabolic signaling, with insulin and leptin signaling being well studied. New evidence indicates that PTP1B is also involved in cancer. In the present study, we report on the establishment of a monoclonal antibody specific for catalytic domain of PTP1B (PTP1Bc) generated through the hybridoma method. The monoclonal antibody is measured to have a titer of 4.1×10(6) against PTP1Bc in indirect ELISA. Western blot and immunofluorescent analyses indicated that this antibody can specifically combine native PTP1B in MDA-MB-231 and MDA-MB-453 cells. This monoclonal antibody against PTP1Bc can help enhance the understanding of PTP1B-related physiological and pathological mechanisms and may act as a therapeutic agent for diabetes, obesity, and cancer in the future.
  Hybridoma (2005) 06/2012; 31(3):209-13. · 0.42 Impact Factor
• Article: Novel superparamagnetic iron oxide nanoparticles for tumor embolization application: preparation, characterization and double targeting.

  Xiaoli Chen, Haiyan Lv, Min Ye, Shengyu Wang, Erru Ni, Fanwei Zeng, Chang Cao, Fanghong Luo, Jianghua Yan
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  ABSTRACT: The goal of this study was to develop novel embolic nanoparticles for targeted tumor therapy with dual targeting: magnetic field-guided and peptide-directed targeting. The embolic nanoparticles SP5.2/tTF-OCMCs-SPIO-NPs were prepared by surface-modifying of superparamagnetic iron oxide nanoparticles (SPIO-NPs) with o-carboxymethylchitosans (OCMCs) and SP5.2/tTF (SP5.2: a peptide binding to VEGFR-1; tTF: truncated tissue factor) to improve their stability and to target over-expressing VEGFR-1 cells. The physicochemical characterization results showed that the OCMCs-SPIO-NPs have a spherical or ellipsoidal morphology with an average diameter of 10-20 nm. And they possess magnetism with a saturation magnetization of 66.1 emu/g, negligible coercivity and remanence at room temperature. In addition, the confocal microscopy, Prussian blue staining and FX activation analysis respectively demonstrated the peptide-directed targeting, magnetic field-guided targeted and blood coagulation activity of the SP5.2/tTF-OCMCs-SPIO-NPs. These properties separately belong to SP5.2, Fe(3)O(4) and tTF moieties of the SP5.2/tTF-OCMCs-SPIO-NPs. Thus these SP5.2/tTF-OCMCs-SPIO-NPs with double-targeting function should have a potential application in embolization therapy of tumor blood vessels.
  International journal of pharmaceutics 04/2012; 426(1-2):248-55. · 2.96 Impact Factor
• Article: Monoclonal antibody against NRP-1 b1b2.

  Xiang Li, Fanghong Luo, Shengyu Wang, Erru Ni, Xiaoyong Tang, Haiyan Lv, Xiaoli Chen, Lukui Chen, Jianghua Yan
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  ABSTRACT: Neuropilin-1 is a member of the neuropilins family protein, which contains a large extracellular domain (a1a2, b1b2 and c), a single transmembrane domain, and a short cytoplasmic tail. NRP-1 plays a critical role in angiogenesis and stimulates endothelial cell division and migration by binding VEGF(165) with b1b2 domain. In the present study, we report the establishment of a monoclonal antibody (A6-26-11-26 clone) specific for NRP-1 b1b2 through hybridoma method. Western blot analysis indicates that our NRP-1 b1b2 MAb can combine both NRP-1 b1b2 and NRP-1 originated from tumor cells. This monoclonal antibody against NRP-1 b1b2 will be useful in the further development of cancer target strategy.
  Hybridoma (2005) 08/2011; 30(4):369-73. · 0.42 Impact Factor
• Conference Proceeding: Influencing Mechanism of Apparent Space Dimensions on Interface Aesthetics and Apparent Usability.

  Tian Lei, Yingbin Zhou, Xiang Li, Xiaoli Chen
  Design, User Experience, and Usability. Theory, Methods, Tools and Practice - First International Conference, DUXU 2011, Held as Part of HCI International 2011, Orlando, FL, USA, July 9-14, 2011, Proceedings, Part II; 01/2011