Keyla Cristiny da Silva Gonçalves

Publications (1)2.15 Total impact

• Source

  Available from: André Luis Souza dos Santos

  Article: Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes.

  Fernanda de Aquino Marinho, Keyla Cristiny da Silva Gonçalves, Selma Soares de Oliveira, Ana-Carolina de Siqueira Couto de Oliveira, Maria Bellio, Claudia Masini d'Avila-Levy, André Luis Souza dos Santos, Marta Helena Branquinha
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  ABSTRACT: In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.
  Memórias do Instituto Oswaldo Cruz 06/2011; 106(4):507-9. · 2.15 Impact Factor