Kaori Otani

Hokkaido University, Sapporo, Hokkaidō, Japan

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Publications (3)3.43 Total impact

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    ABSTRACT: Background and Objective Beta-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three-dimensional scaffold, using β-TCP, for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of implantation of the PLGA/β-TCP scaffold loaded with fibroblast growth factor-2 (FGF-2) on bone augmentation.Material and Methods The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by scanning electron miscroscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction, compressive testing, cell culture and a subcutaneous implant test. Subsequently, a bone-forming test was performed using 52 rats. The β-TCP scaffold, PLGA-coated scaffold, and β-TCP and PLGA-coated scaffolds loaded with FGF-2, were implanted into rat cranial bone. Histological observations were made at 10 and 35 d postsurgery.ResultsSEM and TEM observations showed a thin PLGA layer on the β-TCP particles after coating. High porosity (> 90%) of the scaffold was exhibited after PLGA coating, and the compressive strength of the PLGA/β-TCP scaffold was six-fold greater than that of the noncoated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples obtained following implantation of PLGA/β-TCP scaffold loaded with FGF-2 showed significant bone augmentation.Conclusion The PLGA coating improved the mechanical strength of β-TCP scaffolds while maintaining high porosity and tissue compatibility. PLGA/β-TCP scaffolds, in combination with FGF-2, are bioeffective for bone augmentation.
    Journal of Periodontal Research 06/2014; 50(2). DOI:10.1111/jre.12206 · 2.47 Impact Factor
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    ABSTRACT: We prepared highly porous beta-tricalcium phosphate (β-TCP) scaffolds with different open-cell structure sizes. The aim of this study was to examine whether the open-cell size of the scaffold affected osteoinduction in combination with fibroblast growth factor-2 (FGF2) in rats. Polyurethane foam was immersed in β-TCP slurry and sintered in a furnace. Porous β-TCP scaffolds were prepared in three cell sizes (0.6, 0.4 and 0.3 mm) and characterized. Subsequently, each scaffold with FGF2 was implanted to rat cranial bone. Histomorphometric analyses were taken at 35 days post-surgery. The results showed that each β-TCP scaffold exhibited fully interconnected porosity, and frequently allowed bone tissue ingrowth. The 0.4-mm cell sized scaffold significantly promoted bone augmentation compared to the 0.3-mm type. Resorption of the β-TCP scaffold of 0.4-mm cell size was frequently accelerated. In conclusion, FGF2-loaded β-TCP scaffolds with 0.4-mm cell size would be effective for bone tissue engineering.
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    ABSTRACT: This study evaluated apical periodontal healing after root-end sealing using 4-META/MMA-TBB resin (SB), and root-end filling using reinforced zinc oxide eugenol cement (EBA) or mineral trioxide aggregate (MTA) when root canal infection persisted. Apical periodontitis was induced in mandibular premolars of beagles by contaminating the root canals with dental plaque. After 1 month, in the SB group, SB was applied to the resected surface following apicoectomy. In the EBA and MTA groups, a root-end cavity was prepared and filled with EBA or MTA. In the control group, the root-end was not filled. Fourteen weeks after surgery, histological and radiographic analyses in a beagle model were performed. The bone defect area in the SB, EBA and MTA groups was significantly smaller than that in the control group. The result indicated that root-end sealing using SB and root-end filling using EBA or MTA are significantly better than control.
    Dental Materials Journal 07/2011; 30(4):485-92. DOI:10.4012/dmj.2010-158 · 0.97 Impact Factor

Publication Stats

10 Citations
3.43 Total Impact Points


  • 2011-2014
    • Hokkaido University
      • Department of Periodontology and Endodontology
      Sapporo, Hokkaidō, Japan