Juliane Caroline Leão

Publications (3)5.23 Total impact

• Article: Zymographic and ultrastructural evaluations after low-level laser irradiation on masseter muscle of HRS/J strain mice.

  Mamie Mizusaki Iyomasa, Elen Camargo Rizzi, Juliane Caroline Leão, João Paulo Mardegan Issa, Fernando José Dias, Yamba Carla Lara Pereira, Maria José Vieira Fonseca, Fabiana Testa Moura de Carvalho Vicentini, Ii-Sei Watanabe
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  ABSTRACT: Low-level laser therapy (LLLT) has been widely used in the treatment of the stomatognathic system dysfunction; however, its biological effect remains poorly understood. This study evaluated the effect of LLLT (GaAlAs, 780 nm, 20 J/cm², 40 mW) on masseter muscle of HRS/J mice after different numbers of laser irradiations (three, six, and ten) for 20 s in alternate days. Three experimental groups were defined according to the number of laser irradiations and three control groups (n = 5) were used. On the third day after the last irradiation, all animals were killed and the masseter muscle was removed and processed for the following analysis: (a) transmission electron microscopy, (b) zymography, (c) immunohistochemistry for vascular endothelial growth factor (VEGF) and VEGFR-2. The results showed: (a) with six laser applications, a dilation of T tubules, and sarcoplasmic reticulum cistern, increased pinocytosed vesicles in the endothelium; with ten laser applications, few pinocytic vesicles in the endothelium and condensed mitochondria. (b) Under the conditions of this study, the synthesis of other matrix metalloproteinases was not observed, only the MMP-2 and -9. (c) After ten laser irradiations, immunostaining was observed only for VEGFR-2. We conclude that after six laser applications, ultrastructural changes may facilitate the Ca(+2) transfer to cytosol and increase the fluid transport from one surface to another. The ultrastructural changes and no immunostaining for VEGF with ten applications may decrease the metabolic activity as well as damage the angiogenic process, suggesting that an effective number of laser applications may be less than ten, associating to this therapy a better cost-benefit.
  Lasers in Medical Science 07/2012; · 2.00 Impact Factor
• Article: Histomorphological and Angiogenic Analyzes of Skin Epithelium After Low Laser Irradiation in Hairless Mice

  Juliane Caroline Leão, João Paulo Mardegan Issa, Dimitrius Leonardo Pitol, Ellen Camargo Rizzi, Fernando José Dias, Selma Siéssere, Simone Cecílio Hallak Regalo, Mamie Mizusaki Iyomasa
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  ABSTRACT: It is not well-understood how low-laser therapy affects the skin of the applied area. This study analyzes skin of the masseteric region of mice from the HRS/J strain after three different application regimens (three, six or ten applications per regimen) of low intensity laser at 20 J/cm2 and 40 mW for 20 sec on alternate days. Three experimental groups according to the number of laser applications (three, six or ten) and three control groups (N = 5 animals for each group) were used. On the third day after the last irradiation, all animals were sacrificed and the skin was removed and processed to analyze the relative occupation of the test area by each epithelial layer and the aspects of neovascularization. Data were submitted to statistical analyzes. The irradiated groups compared to their respective controls at each period of time, showed no significant difference in relative occupation of the test area by the layers and epithelium areas for three and six applications, but for ten applications, a significant decrease (P < 0.05) in the basal and granulosum layers, and epithelium areas were found. From the comparisons of the three irradiated groups together, the group with six laser applications showed statistical difference (P < 0.05) in total epithelium and on the layers. Vascular endothelial growth factor (VEGF) and VEGFR-2 immunoreactivities were similar for the control and irradiated groups. Results suggested a biostimulatory effect with low risks associated with superficial tissues, when the treatment aims the deeper layers after six applications. Anat Rec, 2011. © 2011 Wiley-Liss, Inc.
  The Anatomical Record Advances in Integrative Anatomy and Evolutionary Biology 07/2011; 294(9):1592 - 1600. · 1.47 Impact Factor
• Article: Low-level laser intensity application in masseter muscle for treatment purposes.

  Élen Camargo Rizzi, João Paulo Mardegan Issa, Fernando José Dias, Juliane Caroline Leão, Simone Cecílio Hallak Regalo, Selma Siéssere, Ii-sei Watanabe, Mamie Mizusaki Iyomasa
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  ABSTRACT: This study evaluated with histochemical analysis how the number of laser applications can affect the masseter muscle. In dentistry today, the laser is used in patients with temporomandibular disorders (TMDs), mainly for radiating pain in the masticatory muscles, whose origins may be associated with malocclusion, although the laser effects are not well understood on the cellular level. Thirty mice (HRS/J lineage) were randomly distributed into groups according to the number of laser applications (three, six, and 10). For each group of laser applications (experimental, n = 5), it was considered the control group (n = 5), which was not irradiated. All animals inhaled halothane (2-bromo-2-chloro-1, 1, 1-trifluoroethane, minimum 99%, Sigma Aldrich, India) before each laser irradiation performed on the left masseter muscle region, on alternate days with 20 J/cm(2), 40 mW, for 20 sec. The muscle samples were collected for histochemical analysis with succinate dehydrogenase (SDH) enzyme 72 h after the last application. (a) A decrease in area of light fibers type (35.91% ± 6.9%; 32.08% ± 6.3%, and 27.88% ± 6.3%), according to the increase of laser applications (p < 0.05); (b) significant increase (p < 0.05) in the area of intermediate fibers, with an increase of laser application (11.08% ± 3.9%; 16.52% ± 5.7%, and 15.96% ± 3.9%), although the increase with 10 applications was small; (c) area increase of dark fibers in the group with three laser applications (0.16% ± 0.3%) (p < 0.05), and in groups with six and 10 laser applications, respectively (9.68% ± 6.0% and 9.60% ± 4.0%). The SDH enzyme activity revealed that the number of laser applications increases the metabolic pattern of the muscle fibers. A minimal difference in metabolic activity between six and 10 applications of a laser suggests that further analyses should be done to confirm that six applications are enough to produce the same clinical effects, thereby contributing data to professionals from different fields in regard to the cost-benefit ratio of this therapy.
  Photomedicine and laser surgery 10/2010; 28 Suppl 2:S31-5. · 1.76 Impact Factor