Jia-Xuan Zhang

Publications (2)2.86 Total impact

• Article: Cloning, soluble expression, rapid purification and characterization of human Cofilin1.

  Jia-Xin Lu, Yang-Fei Xiang, Jia-Xuan Zhang, Huai-Qiang Ju, Zhen-Ping Chen, Qiao-Li Wang, Wei Chen, Xin-Lei Peng, Bo Han, Yi-Fei Wang
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  ABSTRACT: Cofilin1 is an actin-binding protein that plays a critical role in the regulation of actin cytoskeleton and consequently affects various physiological processes. In this study, the human Cofilin1 cDNA was cloned into the expression vector pET-28a(+) with a 6 × His tag and expressed as soluble protein in Escherichia coli BL21(DE3). Approximately 78 mg of Cofilin1, which showed high activity as determined by native PAGE, could be purified from each liter of LB medium by His-tag affinity chromatography and gel filtration. Further, high-titer IgG against Cofilin1 was positively detected after immunization in rabbits and the polyclonal antibodies were purified and identified. Together, this report provides the first protocol to efficiently obtain human Cofilin1 with high biological activity and immunogenicity using E. coli BL21 (DE3) expression system.
  Protein Expression and Purification 03/2012; 82(1):186-91. · 1.59 Impact Factor
• Article: SNX-2112, a novel Hsp90 inhibitor, induces G2/M cell cycle arrest and apoptosis in MCF-7 cells.

  Shao-Xiang Wang, Huai-Qiang Ju, Kai-Sheng Liu, Jia-Xuan Zhang, Xiao Wang, Yang-Fei Xiang, Rui Wang, Jin-Yun Liu, Qiu-Ying Liu, Min Xia, Guo-Wen Xing, Zhong Liu, Yi-Fei Wang
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  ABSTRACT: SNX-2112 is a heat shock protein 90 (Hsp90) inhibitor with anticancer properties currently in clinical trials. This study investigated the effects of SNX-2112 on inhibition of cell growth, the cell cycle, and apoptosis in MCF-7 human breast cancer cells, in addition to the various molecular mechanisms. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometric analysis suggest that SNX-2112 inhibits cell growth in a time- and dose-dependent manner more potently than 17-(allylamino)-17-demethoxygeldanmycin (17-AAG), a traditional Hsp90 inhibitor, probably as a result of cell-cycle arrest at the G2/M phase and the induction of apoptosis. Downregulation of Bcl-2 and Bcl-xL, upregulation of Bax, cleavage of caspase-9 and poly (ADP-ribose) polymerase (PARP), and degradation of the breast cancer-related Hsp90 client proteins human epidermal growth factor receptor-2 (HER2), Akt, Raf-1, and nuclear factor kappa-B kinase (IKK) were observed in SNX-2112 treated cells by Western blot assay. These findings suggest that the molecular mechanisms of cell-growth inhibition by SNX-2112 involve activation of the mitochondrial apoptotic pathway and the degradation of breast cancer-related proteins.
  Bioscience Biotechnology and Biochemistry 08/2011; 75(8):1540-5. · 1.28 Impact Factor