Honglie Hou

Publications (2)4.73 Total impact

• Article: Cloning and characterization of telomerase reverse transcriptase gene in Trichinella spiralis.

  Yanan Cai, Yongxing Ai, Quan Zhao, Jianhua Li, Guilian Yang, Pengtao Gong, Qiuyue Wang, Honglie Hou, Guocai Zhang, Lingdan Li, Ju Yang, He Li, Jun Zheng, Shuhong Li, Xichen Zhang
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  ABSTRACT: The telomerase reverse transcriptase (TERT) is primarily known for its ability to elongate telomeres for maintaining chromosomal integrity and delaying cellular senescence. It plays an important role in cell proliferation, differentiation, tumorigenesis, and aging. Telomerase includes two core components-an internal RNA moiety acting as a template of DNA extension and a catalytic subunit (TERT) which provides catalytic activity. Here, we described the cloning, sequence, and characterization of the TERT gene from Trichinella spiralis (T. spiralis). The prediction results of amino acid sequence showed that it possessed all the motifs characteristics of the TERT family members. T. spiralis TERT (Ts_TERT) cDNA contains an open reading frame encoding a protein with 1,201 amino acids with moleculer mass of 139 kDa and isoelectric point of 9.673, and the protein contains the conserved reverse transcriptase motifs 1, 2, A, B, C, D, and E, as well as the TERT-specific T motifs and the N-terminal conserved motifs GQ, CP, and QFP. While RT-PCR analysis indicates that TERT mRNA is expressed in T. spiralis adult worm, newborn larvae, and muscle larvae.
  Parasitology Research 07/2011; 110(1):411-7. · 2.15 Impact Factor
• Article: Prevalence of Dirofilaria immitis infection in dogs from Dandong, China.

  Honglie Hou, Guoshun Shen, Wei Wu, Pengtao Gong, Quan Liu, Juan You, Yanan Cai, Jianhua Li, Xichen Zhang
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  ABSTRACT: The aim of the present study was to estimate the prevalence and risk factors of Dirofilaria immitis infection in dogs from Dandong, China. A total of 886 dogs were examined for D. immitis infection by microscopic examination and PCR, indicating that the prevalence was 16.6% (213/886) and 24.0% (147/886), respectively. The odds of infection were significantly higher in older dogs and dogs sheltered in outdoor, compared to the younger ones and ones sheltered in indoor. No significant difference of infection was observed in different genders, and between pure breed and cross-breed dogs in the same rearing conditions. These results indicated that the risk of exposure to D. immitis in dogs is high in Dandong, China, and prophylaxis against the parasite is advisable to decrease the incidence of canine dirofilariosis.
  Veterinary Parasitology 06/2011; 183(1-2):189-93. · 2.58 Impact Factor