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A.A. Akhavan,
M.R. Yaghoobi-Ershadi,
A. Khamesipour, H. Mirhendi,
M.H. Alimohammadian,
Y. Rassi,
M.H. Arandian,
R. Jafari,
H. Abdoli,
N. Shareghi,
M. Ghanei,
N. Jalali-zand
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ABSTRACT: Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is a great public health problem in the Old World. Leishmania major is widely distributed in populations of rodents in arid and savannah regions. In this study, seasonal variation of natural
infection with Leishmania parasites in Rhombomys opimus (Rodentia: Gerbillinae) population of an endemic focus of ZCL in Iran was monitored. The study was conducted from October
2007 to October 2008 in the central part of the country. Nested polymerase chain reaction (PCR) assay was used for the detection
and identification of Leishmania parasites, and the results were confirmed by PCR-restriction fragment length polymorphism (RFLP). The results showed that
Leishmania infection rate was 55.8% (29 out of 52 gerbils) using nested PCR. The highest and lowest Leishmania infection rates were observed in fall and summer, respectively. Gerbils that were found to be infected only with L. major were 5.8%, and that with Leishmania turanica were 23.1%. A mixed natural infection was seen in the rodents with L. major and L. turanica (21.2%), with L. major and L. gerbilli (1.9%), and with all the three species (3.9%). Leishmania major infection alone was seen in fall and winter whereas mixed infection of L. major and L. turanica was observed in all seasons except in summer. Leishmania turanica infection was observed throughout the year. It is concluded that L. major, L. gerbilli, and L. turanica circulate in the population of R. opimus in central part of Iran. Leishmania major infection is usually accompanied by L. turanica in naturally infected gerbils with the highest rate in fall. It is recommended that the role of L. turanica in the epidemiology and transmission of ZCL should be reconsidered.
La leishmaniose cutanée zoonotique (LCZ) due à Leishmania major constitue un problème de santé publique dans l’ancien monde. L. major est une espèce largement distribuée dans les populations de rongeurs vivant dans les régions arides et de savane. Dans la
présente étude, la variation saisonnière de l’infection naturelle par les parasites du genre Leishmania chez la Grande Gerbille Rhombomys opimus (Rodentia: Gerbillinae) au sein d’une population d’un foyer endémique de LCZ en Iran a été contrôlée. L’étude a été menée
d’octobre 2007 à octobre 2008 dans la partie centrale de l’Iran. Une PCR nichée a été utilisée pour la détection et l’identification
du parasite Leishmania, et les résultats ont été confirmés par PCR-RFLP. Les résultats ont montré que le taux d’infection par Leishmania était de 55,8 % (29 sur 52 animaux) en utilisant la PCR nichée. Les taux d’infection par Leishmania les plus forts et les plus faibles ont été observés, respectivement, en automne et en été; 5,8 % des gerbilles étaient infectées
par L. major isolément et 23,1% par L. turanica isolément. Une infection mixte naturelle a été observée chez les rongeurs associant L. major et L. turanica (21,2 %), ou associant L. major et L. gerbilli (1,9 %), ou associant les trois espèces (3,9 %). L’infection due à L. major isolément a été observée en automne et en hiver. Une infection mixte associant L. major et L. turanica a été observée en toutes saisons, sauf en été. L’infection à L. turanica a été observée tout au long de l’année. En conclusion, les trois espèces L. major, L. gerbilli et L. turanica circulent dans la population de R. opimus dans la partie centrale de l’Iran. L. major est habituellement accompagnée par L. turanica chez les gerbilles naturellement infectées avec un plus fort taux en automne. Le rôle de L. turanica dans l’épidémiologie et la transmission de la LCZ devrait être reconsidéré.
Keywords
Rhombomys opimus
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Leishmania major
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L. gerbilli
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L. turanica
-Seasonal variation-Zoonotic cutaneous leishmaniasis-Nested PCR-Badrood-Iran-Middle South Asia
Mots clés
Rhombomys opimus
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Leishmania major
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L. gerbilli
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L. turanica
-Variation saisonnière-Leishmaniose cutanée zoonotique-PCR niche-Badrood-Iran-Moyen-Orient
Bulletin de la Société de pathologie exotique 04/2012; 103(2):84-89.
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ABSTRACT: Echinococcus granulosus, the aetiologic agent of cystic echinococcosis (CE), is one of the most important zoonotic helminthes worldwide. Isolates of the parasite show considerable genetic variation in different intermediate hosts. Several genotypes and species are described in different eco-epidemiological settings. This study investigated E. granulosus genotypes existing in livestock and humans from the province of Kerman, located in south-eastern Iran, using sequencing data of cox1 and nad1 mitochondrial genes. Fifty-eight E. granulosus isolates, including 35 from sheep, 11 from cattle, 9 from camels and 3 from goats, were collected from slaughterhouses throughout Kerman. One human isolate was obtained from a surgical case of CE. Mitochondrial cox1 and nad1 regions were amplified using polymerase chain reaction (PCR) and 38 isolates were sequenced. Genotypes G1 (73.7%), G3 (13.2%) and G6 (13.1%) were identified from the isolates. G1 was the most common genotype from sheep (86.7%), cattle (80%), camels (44.4%) and goats (100%). Sheep, cattle and camels were also found to be infected with the G3 genotype (buffalo strain). The human isolate was identified as the G6 genotype. Results showed that the G3 genotype occurred in different animal hosts in addition to G1 and G6 genotypes.
Journal of Helminthology 07/2011; 86(3):263-70. · 1.38 Impact Factor
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ABSTRACT: Different molecular methods for the discrimination of Candida glabrata, C. bracarensis and C. nivariensis were evaluated and the prevalence of these species among Danish blood isolates investigated. Control strains were used to determine fragment length polymorphism in the ITS1, ITS2, ITS1-5.8S-ITS2 regions and in the D1/D2 domain of 26S rDNA using primers designed for this study. A total of 133 blood isolates previously identified as C. glabrata were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH) method. The size of ITS1 allowed differentiation between C. glabrata (483), C. nivariensis (361) and C. bracarensis (385), whereas the ITS2 region was of similar size in C. nivariensis (417) and C. glabrata (418). Sequence analysis of the ITS region suggested that many restriction enzymes were suitable for RFLP differentiation of the species. Enzymatic digestion of the D1/D2 domain with TatI produced unique band sizes for each of the three species. PCR-RFLP and PNA-FISH were in agreement for all of the isolates tested. None of the 133 Danish blood isolates were C. nivariensis or C. bracarensis. Fragment size polymorphism of ITS1 and RFLP of the D1/D2 domain or the ITS region are useful methods for the differentiation of the species within the C. glabrata group. C. bracarensis and C. nivariensis are rare among Danish C. glabrata blood isolates.
European Journal of Clinical Microbiology 05/2011; 30(11):1409-16. · 2.86 Impact Factor
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A A Akhavan,
M R Yaghoobi-Ershadi,
A Khamesipour, H Mirhendi,
M H Alimohammadian,
Y Rassi,
M H Arandian,
R Jafari,
H Abdoli,
N Shareghi,
M Ghanei,
N Jalali-zand
[show abstract]
[hide abstract]
ABSTRACT: Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is a great public health problem in the Old World. Leishmania major is widely distributed in populations of rodents in arid and savannah regions. In this study, seasonal variation of natural infection with Leishmania parasites in Rhombomys opimus (Rodentia: Gerbillinae) population of an endemic focus of ZCL in Iran was monitored. The study was conducted from October 2007 to October 2008 in the central part of the country. Nested polymerase chain reaction (PCR) assay was used for the detection and identification of Leishmania parasites, and the results were confirmed by PCR-restriction fragment length polymorphism (RFLP). The results showed that Leishmania infection rate was 55.8% (29 out of 52 gerbils) using nested PCR. The highest and lowest Leishmania infection rates were observed in fall and summer, respectively. Gerbils that were found to be infected only with L. major were 5.8%, and that with Leishmania turanica were 23.1%. A mixed natural infection was seen in the rodents with L. major and L. turanica (21.2%), with L. major and L. gerbilli (1.9%), and with all the three species (3.9%). Leishmania major infection alone was seen in fall and winter whereas mixed infection of L. major and L. turanica was observed in all seasons except in summer. Leishmania turanica infection was observed throughout the year. It is concluded that L. major, L. gerbilli, and L. turanica circulate in the population of R. opimus in central part of Iran. Leishmania major infection is usually accompanied by L. turanica in naturally infected gerbils with the highest rate in fall. It is recommended that the role of L. turanica in the epidemiology and transmission of ZCL should be reconsidered.
Bulletin de la Société de pathologie exotique 04/2010; 103(2):84-9.
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Aa Akhavan,
Mr Yaghoobi-Ershadi, H Mirhendi,
Mh Alimohammadian,
Y Rassi,
N Shareghi,
R Jafari,
Mh Arandian,
H Abdoli,
M Ghanei,
N Jalali-Zand,
A Khamesipour
[show abstract]
[hide abstract]
ABSTRACT: Background: Zoonotic cutaneous leishmaniasis (ZCL) is an expanding disease and public health problem in Iran. In the current study, natural Leishmania infection rate and seasonal fluctuation of the infection in Rhombomys opimus population of a hyperendemic focus of ZCL in Iran was investigated. Methods: The study was conducted from October 2006 to October 2008 in Esfahan Province, central part of Iran. An extensive sampling of rodents using Sherman traps was done in different seasons. Nested PCR assay was used for detection and identification of Leishmania species and the results were confirmed using PCR-RFLP. Results: Leishmania infection rate was 58.6% (34 of 58) using nested PCR. 44.8% of the gerbils were infected only with L. turanica and 1.7% with L. gerbilli alone. A mixed natural infection with L. major and L. turanica was seen in 12.1% of the rodents. L. major infection alone was not seen in R. opimus population in the study area. The highest and lowest Leishmania infection rates were observed in fall and spring respectively. L. turanica infection was observed throughout the year whereas mixed infections with L. major and L. turanica was not seen in spring. Conclusion: It is concluded that in the study area, L. major, L. gerbilli and L. turanica circulate in the population of R. opimus. Leishmania major infection usually accompanied by L. turanica in naturally infected gerbils with the highest rate in fall. It is recommended that the role of L. turanica in the epidemiology and transmission of ZCL be revisited.
Iranian Journal of Public Health 01/2010; 39:1-7. · 0.38 Impact Factor
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ABSTRACT: Trichinella larvae isolated from the thigh muscle of a wild boar, Sus scrofa, captured from Gilan Province, northern Iran, was processed for DNA analysis. Polymerase chain reaction (PCR) for amplification of the 5S rDNA fragment demonstrated a 700 bp band on agarose gel. Analysis of DNA sequencing by BLAST confirmed the isolate as T. murrelli. This report constitutes the first recorded occurrence of T. murrelli in Asia, and also the first occurrence in a wild boar host.
Journal of Helminthology 07/2009; 83(4):399-402. · 1.38 Impact Factor
