Eun Jung Lee

Yonsei University Hospital, Sŏul, Seoul, South Korea

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Publications (176)495.77 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Expression of insulin-like growth factor 1 receptor (IGF-1R) in non-small cell lung cancer (NSCLC) is associated with poor prognosis. The IGF-1R pathway activates downstream targets that bypass dependency in signals from the epidermal growth factor receptor (EGFR), which mediates resistance to EGFR tyrosine kinase inhibitors (TKIs). The aim of the present study was to determine the predictive role of IGF-1R expression in the response to EGFR-TKIs of NSCLC patients harboring activating EGFR mutations.
    Lung Cancer. 01/2015;
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    ABSTRACT: Considering the problems of small interfering RNA (siRNA) delivery using traditional viral and nonviral vehicles, a new siRNA delivery system to enhance efficiency and safety needs to be developed. Here human ferritin-based proteinticles are genetically engineered to simultaneously display various functional peptides on the surface of proteinticles: cationic peptide to capture siRNA, tumor cell targeting and penetrating peptides, and enzymatically cleaved peptide to release siRNA inside tumor cell. In the in vitro treatment of poly-siRNA-proteinticle complex, both of the tumor cell targeting and penetrating peptides are important for efficient delivery of siRNA, and the red fluorescent protein (RFP) expression in RFP-expressing tumor cells is notably suppressed by the delivered siRNA with the complementary sequence to RFP mRNA. It seems that the human ferritin-based proteinticle is an efficient, stable, and safe tool for siRNA delivery, having a great potential for application to in vivo cancer treatment. The unique feature of proteinticles is that multiple and functional peptides can be simultaneously and evenly placed and also easily switched on the proteinticle surface through a simple genetic modification, which is likely to make proteinticles appropriate for targeted delivery of siRNA to a wide range of cancer cells.
    Advanced Functional Materials 01/2015; · 10.44 Impact Factor
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    ABSTRACT: nodal dissemination of MALT lymphoma can mimic nodal lymphoma, especially when the primary site is grossly indistinct. Plasmacytic differentiation may often be present with MALT lymphoma; an associated extreme form such as Mott cell formation, which strongly suggests PCN or LPL, may also be observed in MALT lymphoma. An exhaustive evaluation, including PET-CT, protein electrophoresis, and immunohistochemical staining, should be conducted for an accurate diagnosis
    Blood Research. 12/2014; 49:275-278.
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    ABSTRACT: Background: Klebsiella pneumoniae is an uncommon cause of community-acquired bacterial meningitis. The clinical and laboratory characteristics of community-acquired K. pneumoniae meningitis (CA-KPM) remain to be elucidated. In this study, we compared the clinical and laboratory features, and outcomes, of CA-KPM with those of community-acquired Streptococcus pneumoniae meningitis (CA-SPM). Methods: This multi-center, retrospective cohort study was performed at 8 general hospitals in the Republic of Korea. Using a clinical microbiology computerized database, all patients whose cerebrospinal fluid (CSF) cultures yielded K. pneumoniae or S. pneumoniae between January 1997 and March 2013 were identified. Adult patients with clinical meningitis were identified by detailed review of medical records. Results: A total of 83 patients, 27 with CA-KPM and 56 with CA-SPM, were included. The proportion of males (KPM, 55.6% vs. SPM, 55.4%, P = 0.99) and median age (KPM, 59 year vs. 59 year, P = 0.23) were not significantly different between the groups. Diabetes mellitus (KPM, 48.1% vs. SPM, 21.4%; P = 0.01) and liver cirrhosis (KPM, 22.2% vs. SPM, 5.4%; P = 0.05) were more common in the CA-KPM group. Although the profiles of CSF analyses did not differ significantly between the groups, comatose mentality was more frequent in the CA-KPM group (KPM, 40.7% vs. SPM, 12.5%; P = 0.01). Septic shock (KPM, 44.4% vs. SPM, 89%; P < 0.001) and concomitant extrameningeal infection were also more common in the CA-KPM group (KPM, 37% vs. SPM, 7.1%; P = 0.001). Vancomycin plus cefotaxime/ceftriaxone was the most frequently used initial empirical therapy (KPM, 74.1% vs. SPM, 85.7%; P = 0.23) in both groups. However, 28-day mortality (KPM, 44.4% vs. SPM, 10.7%; P < 0.001) and in-hospital mortality (KPM, 51.9% vs. SPM, 14.3%; P < 0.001) were higher in the CA-KPM group. Conclusion: Diabetes mellitus and liver cirrhosis are more common in K. pneumoniae meningitis than S. pneumoniae meningitis, and the former is more likely to present severe manifestations and poor outcomes.
    IDWeek 2014 Meeting of the Infectious Diseases Society of America; 10/2014
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    ABSTRACT: “Proteinticle” is nano-scale protein particle that is self-assembled inside cells with constant 3D structure and surface topology. The binding of IgG to B domain of Staphylococcal protein A (SPAB) molecules that are genetically inserted into the surface of proteinticle enables the variable domains of bound IgG to be well oriented to effectively capture antigens, accordingly forming a highly sensitive 3D IgG probe. The five different proteinticles that originate from human, bacteria, and virus and totally differ in size, shape, and surface structure were used for the surface display of SPAB. The dissociation constant (KD) in the binding of IgG to SPAB on the proteinticle surface was estimated based on Langmuir adsorption isotherm model: KD was 1-3 orders-of-magnitude lower compared to the previously reported KD in the binding of IgG to Staphylococcal protein A. The surface density and distribution of SPAB and especially the existence of hot (or highly congested) spots of SPAB, which depend on surface structure and number of subunits as well as size and shape of proteinticle, is of crucial importance for the effective binding of IgG to SPAB on proteinticles. Although the five different proteinticles were demonstrated as proof-of-concept here, SPAB-mediated immobilization of IgG on the other proteinticles would be very useful for the fabrication of sensitive 3D immunoassay platforms.
    Nanoscale 10/2014; · 6.74 Impact Factor
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    ABSTRACT: Non-destructive continuous monitoring of regenerative tissue is required throughout the entire periodof in vitro tissue culture. Microscopic electrical impedance tomography (micro-EIT) has the potentialto monitor the physiological state of tissues by forming three-dimensional images of impedancechanges in a non-destructive and label-free manner. We developed a new micro-EIT system andreport on simulation and experimental results of its macroscopic model.
    BioMedical Engineering OnLine 10/2014; 13(1):142. · 1.75 Impact Factor
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    ABSTRACT: Retinal ganglion cell (RGC) axons of binocular animals cross the midline at the optic chiasm (OC) to grow toward their synaptic targets in the contralateral brain. Ventral anterior homeobox 1 (Vax1) plays an essential role in the development of the OC by regulating RGC axon growth in a non-cell autonomous manner. Here, we identify an unexpected function of Vax1 that is secreted from ventral hypothalamic cells and diffuses to RGC axons, where it promotes axonal growth independent of its transcription factor activity. We demonstrate that Vax1 binds to extracellular sugar groups of the heparan sulfate proteoglycans (HSPGs) located in RGC axons. Both Vax1 binding to HSPGs and subsequent penetration into the axoplasm, where Vax1 activates local protein synthesis, are required for RGC axonal growth. Together, our findings demonstrate that Vax1 possesses a novel RGC axon growth factor activity that is critical for the development of the mammalian binocular visual system.
    eLife Sciences 09/2014; · 8.52 Impact Factor
  • Ontology & Neurotology 08/2014; · 1.60 Impact Factor
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    ABSTRACT: Two different protein nanoparticles that are totally different in shape and surface structure, i.e. Escherichia coli DNA-binding protein (eDPS) (spherical, 10 nm) and Thermoplasma acidophilum proteasome (tPTS) (cylindrical, 12 × 15 nm) were engineered for in vivo optical tumor detection: arginine–glycine–aspartic acid (RGD) peptide (CDCRGDCFC) was genetically inserted to the surface of each protein nanoparticle, and also near-infrared fluorescence dye was chemically linked to the surface lysine residues. The specific affinity of RGD for integrin (αvβ3) facilitated the uptake of RGD-presenting protein nanoparticles by integrin-expressing tumor cells, and also the protein nanoparticles neither adversely affected cell viability nor induced cell damage. After intravenously injected to tumor-bearing mice, all the protein nanoparticles successfully reached tumor with negligible renal clearance, and then the surface RGD peptides caused more prolonged retention of protein nanoparticles in tumor and accordingly higher fluorescence intensity of tumor image. In particular, the fluorescence of tumor image was more intensive with tPTS than eDPS, which is due presumably to longer in vivo half-life and circulation of tPTS that originates from thermophilic and acidophilic bacterium. Although eDPS and tPTS were used as proof-of-concept in this study, it seems that other protein nanoparticles with different size, shape, and surface structure can be applied to effective in vivo tumor detection.
    Biomaterials 08/2014; 35(24):6422–6429. · 8.31 Impact Factor
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    ABSTRACT: PGCS-NPs (40 nm) with excellent photo-thermal activity are developed, on the surface of which affibody peptides with specific affinity for EGFR and many small gold dots (1-3 nm) are densely presented. The IV-injected PGCS-NPs into EGFR-expressing tumor-bearing mice successfully perform targeted and photothermal therapy of cancer. It seems that the small gold dots released from disassembled PGCS-NPs are easily removed and never cause in vivo toxicity problems.
    Advanced Materials 07/2014; · 15.41 Impact Factor
  • Jin Sung Kim, Yong Joo Yoon, Eun Jung Lee
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    ABSTRACT: Abstract Conclusion: Antimicrobial agents (AMAs) and mast cells in chronic otitis media (COM) may play a protective role in the pathogenesis of COM. Among them, our results indicated that lysozyme may be the most important parameter of the mucosa infectivity in COM. Objectives: AMAs include alpha 1-antitrypsin (α1-AT), lysozyme, and lactoferrin and exhibit innate immune activity that is known to play a critical role in mucosal defenses. In addition, proteases expressed in mast cells contribute to mucosal defenses. The aim of this study was to identify AMAs responsible for COM as well as their association with middle ear mucosal diseases. Methods: Middle ear mucosa (MEM) was intraoperatively collected by biopsy from patients, diagnosed as COM without or with cholesteatoma, and immediately processed for immunohistochemical study to evaluate expression of AMAs and mast cell proteases (chymase and tryptase). Results: The AMAs and mast cell enzymes were observed prominently in the chronic middle ear diseases whereas immunoreactivity of α1-AT and lysozyme was not significantly increased in the COM with cholesteatoma compared with that in COM without cholesteatoma. Lactoferrin was not detected in the diseased MEMs.
    Acta Oto-Laryngologica 06/2014; · 0.99 Impact Factor
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    ABSTRACT: Lipofibromatous hamartomas (LFHs) are rare benign tumors that typically present as slow-growing firm masses in the subcutaneous soft tissue of the extremities. These often develop in young adults on the volar aspects of the hands, wrists, and forearms along major nerves and their branches. Only a single case of LFH growth along a cranial nerve has been reported.A 41-year-old man presented with a subcutaneous soft mass of the cheek. Histologically, the mass consisted of a proliferating fibrofatty tissue that surrounded and infiltrated the nerve structure and it was confirmed to be an LFH. After surgical excision, the patient experienced mild facial numbness and motor disturbance on the side ipsilateral to the resection. Because complete excision of the mass can cause sensory or motor disturbances, an accurate diagnosis and proper subsequent treatment, such as partial decompression, are necessary.
    Journal of Craniofacial Surgery 06/2014; 25(4):1553-1554. · 0.68 Impact Factor
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    ABSTRACT: Background: We recently showed that acute cholesterol depletion in the plasma membrane of NCI-H292 cells by methyl-β-cyclodextrin suppressed IL-1beta‐induced MUC5AC gene expression. Because cholesterol depletion is clinically used as an antihypersecretory method, chronic cholesterol depletion by lovastatin is more rational and safe than acute depletion. Therefore, we sought to investigate whether chronic cholesterol depletion by lovastatin is feasible and, if so, suppresses the expression of GMUC5AC in NCI-H292 cells. We also considered whether this alteration of MUC5AC expression is related to IL-1 receptor and mitogen-activated protein kinase (MAPK) activity. Methods: After NCI-H292 cells were pretreated with 10 μM of lovastatin for 1 hour, 10 ng/mL of IL-1β was added and cotreated with lovastatin for 24 hours. MUC5AC mRNA expression was then determined by real-time polymerase chain reaction. Cholesterol depletion by lovastatin was measured by modified microenzymatic fluorescence assay and filipin staining. The phosphorylation of IL-1 receptor, ERK, and p38 MAPK was analyzed by Western blot. Results: Cholesterol in the plasma membrane was significantly depleted by lovastatin treatment for 24 hours. IL-1beta0-induced MUC5AC mRNA expression was decreased by lovastatin and this decrease occurred IL-1 receptor specifically. Lovastatin suppressed the activation of p38 MAPK but not ERK1/2 in cells activated with IL-1beta. This result suggests that lovastatin-mediated suppression of IL-1beta‐induced MUC5AC mRNA operated only viathe p38 MAPK-dependent pathway. Conclusion: Chronic cholesterol depletion in the plasma membrane of NCI-H292 cells may be considered an antihypersecretory method, because it effectively inhibits mucin gene expression of human airway epithelial cells.
    American journal of rhinology & allergy 05/2014; 28(3). · 2.18 Impact Factor
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    ABSTRACT: Abstract Background: Recently, robot-assisted neck dissection (ND) using a transaxillary approach in thyroid cancer patients with lateral neck metastases (LNM) was demonstrated to be feasible. The aim of this study was to compare the surgical outcomes of a modified transaxillary and retroauricular (TARA) versus a conventional transcervical approach in papillary thyroid carcinoma (PTC) patients with LNM. Patients and Methods: In total, 47 patients with PTC underwent total thyroidectomy with central compartment ND and modified radical ND except Level I. Twenty-two NDs were performed via the TARA approach, and 25 unilateral NDs were performed via the conventional transcervical approach. Results: The TARA and the open ND groups consisted of 22 and 25 patients, respectively. The operation time for ND in the TARA group was longer than that in the open ND group (209.4±38.2 minutes versus 143.1±30.5 minutes; P=.000). The mean scar satisfaction score in the TARA group was higher than in the conventional ND group (3.9±1.0 versus 2.8±1.0; P=.000). There were no differences in the mean number of retrieved lymph nodes. Conclusions: The robot-assisted ND via the TARA approach can be an alternative option that produces excellent esthetic results for the management of LNM in PTC patients.
    Journal of Laparoendoscopic & Advanced Surgical Techniques 04/2014; · 1.19 Impact Factor
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    ABSTRACT: Seborrheic keratosis (SK) is common benign epithelial tumor of the skin that can be associated with other cutaneous tumors such as basal cell carcinoma, squamous cell carcinoma and melanoma. On the other hand, melanocytic nevus (MN) is another very common disease, showing anecdotal association with other cutaneous tumors such as trichoepithelioma, syringoma, basal cell carcinoma, trichilemmal cyst and epidermoid cyst. Although it has recently been reported that somatic mutation of BRAF gene is implicated in MN quite frequently, their pathogenic mechanisms, especially the association with other cutaneous tumors, are still elusive. Despite the high frequency of both tumors, however, collision tumors of SK and MN are extremely rare that only a few case reports have been documented so far. Hereby, we report five cases of simultaneous occurrence of SK and MN in 14-year-old female, 36-year-old female, 39-year-old female, 58-year-old male, and 62-year-old male patients. Additional molecular tests for BRAF mutation (V600E) on micro-dissected tissue of the 58-year-old man revealed positivity on the MN and negativity on the SK. Although these results cannot give direct evidence that both tumors have different pathogenic mechanisms, it seems to be more relevant that these collision tumors may occur by chance. Introduction Seborrheic keratosis (SK) is one of the most common benign epithelial tumors of the skin. Despite its frequency, many aspects of SK, especially its pathogenetic mechanism, remain elusive. Not a small number of cutaneous malignant tumors such as basal cell carcinoma, squamous cell carcinoma and melanoma have been documented to be found with SK [1]. Benign tumors that have very occasionally been documented include cutaneous ganglioneuroma, sebaceoma, eccrine poroma and trichilemmoma [1]. However, whether these combined tumors and SK share the same pathogenic mechanism is unclear. Likewise, melanocytic nevus (MN) is another very common disease, showing anecdotal association with other cutaneous tumors such as trichoepithelioma, syringoma, basal cell carcinoma, trichilemmal cyst and epidermoid cyst [1]. Although it has recently been reported that somatic mutation of BRAF gene is implicated in MN quite frequently, their pathogenic mechanisms, especially the association with other cutaneous tumors, are still elusive [2]. Considering the frequency of these two tumors, there have been surprisingly few additional reports of the cases in the literature that SK and MN are combined in the same lesion [3-9]. Some of the cases even showed additional concurrent occurrence of other tumors such as basal cell carcinoma, which suggests the hypothesis that multipotential differentiation capacity of the follicular germ may explain the coexistence of these tumors [6,8].
    Our Dermatology Journal. 04/2014; 5(2):179-182.
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    ABSTRACT: Ginseng is one of the most important folk medicines in the East Asian countries. Ginseng has various bioactive effects on human health including its potential activity of improving the glucose homeostasis and insulin sensitivity. To test the efficacy of ginseng adventitious root extract on hyperglycemia in streptozotocin-induced diabetic rats. Tissue culture raised mountain ginseng adventitious root (TCMGARs) extract enriched with ginsenosides was used as experimental material. Streptozotocin-induced diabetic 'Sprague Dawley' male rats were used as experimental systems and were fed with Tissue culture raised mountain ginseng adventitious root extract. Field cultivated Korean ginseng root extract fed rats were used as positive control and several indices such as body weight, blood glucose level and other serological indicators were tested. Chemical profile showed TCMGARs were rich in varied ginsenosides especially Rb1, Rb2, Rc, Rd, Rg3, and Rh2 when compared to filed cultivated Korean ginseng. TCMGARs extract at dosage levels of 250 and 500mg/kg body weight significantly lowered the blood glucose, total cholesterol and triglyceride content in streptozotocin-induced diabetic rats. The data of in vivo experiments on anti-glycemic effects of TCMGARs proves their efficacy and also their use as dietary supplement.
    Journal of ethnopharmacology 04/2014; · 2.32 Impact Factor
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    ABSTRACT: The Warthin-like variant of papillary thyroid carcinoma (WL-PTC) was first introduced by Apel et al. 1 in 1995 as an extremely rare subtype of papillary thyroid carcinoma (PTC) that is charac-terized by papillary architecture, lined by Hürthle cells, with classical cytologic features of PTC cells, and a heavy lymphocytic stroma of the papillary core. WL-PTC is now classified in the World Health Organization terminology as a special subtype of an oncocytic variant of PTC...
    The Korean Journal of Pathology 04/2014; 48(2):170-3. · 0.17 Impact Factor
  • The Korean Journal of Pathology 04/2014; 48(2):162-3. · 0.17 Impact Factor
  • Yeon Sup Yoon, Yong Joo Yoon, Eun Jung Lee
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    ABSTRACT: Osteomas of the middle ear are rare benign neoplasms. To date, only a few cases have been reported. Osteomas of the middle ear are small, single, usually unilateral, peduncular growths, off-white in color, with a smooth or multilobular surface, asymptomatic or causing functional disorders. The most common symptom is conductive hearing loss because of impingement of the ossicular chain. Some cases are asymptomatic and are diagnosed incidentally. We present two cases of incidentally detected middle ear osteoma. Based on a review of the main articles in the literature and analysis of two cases managed in our department, we describe the clinical spectrum, etiology, and management of middle ear osteomas.
    American journal of otolaryngology 03/2014; · 1.08 Impact Factor
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    ABSTRACT: In the present study, we aimed to investigate anti-obesity effect of CAPE in vivo, and the mechanism by which CAPE regulates body weight in vitro. To confirm anti-obesity effect of CAPE in vivo, mice were fed with a high fat diet (HFD) with different concentrations of CAPE for 5 weeks. CAPE significantly reduced body weight gain and epididymal fat mass in obese mice fed a HFD. In accordance with in vivo results, Oil red O staining results showed that CAPE significantly suppressed MDI-induced adipogenesis of 3T3-L1 preadipocytes. FACS analysis results showed that CAPE delayed MDI-stimulated cell cycle progression, thereby contributing to inhibit mitotic clonal expansion (MCE) which is prerequisite step for adipogenesis. Also, CAPE regulated expression of cyclin D1 and phosphorylation of ERK and Akt which are upstream of cyclin D1. These results suggest that CAPE exerts anti-obesity effect in vivo, presumably through inhibiting adipogenesis at early stage of adipogenesis.
    Journal of Agricultural and Food Chemistry 03/2014; · 3.11 Impact Factor

Publication Stats

1k Citations
495.77 Total Impact Points


  • 2014
    • Yonsei University Hospital
      Sŏul, Seoul, South Korea
  • 2012–2014
    • Chungbuk National University
      • Research Center for the Development of Advanced Horticultural Technology
      Chinsen, North Chungcheong, South Korea
    • Kyung Hee University
      • Department of Medicine
      Sŏul, Seoul, South Korea
  • 2011–2014
    • Korea Advanced Institute of Science and Technology
      • Department of Biological Sciences
      Sŏul, Seoul, South Korea
    • Korea Food Research Institute
      Sŏul, Seoul, South Korea
  • 2009–2014
    • Korea University
      • Department of Chemical and Biological Engineering
      Sŏul, Seoul, South Korea
    • Gyeongsang National University
      • Department of Internal Medicine
      Chinju, South Gyeongsang, South Korea
    • Eulji University
      Daiden, Daejeon, South Korea
  • 2008–2014
    • Chonbuk National University
      Tsiuentcheou, North Jeolla, South Korea
    • Sunchon National University
      • Department of Biology
      Junten, South Jeolla, South Korea
  • 2002–2014
    • Catholic University of Korea
      • • Department of Hospital Pathology
      • • Department of Pediatrics
      • • College of Medicine
      Sŏul, Seoul, South Korea
  • 2013
    • CHA University
      • School of Medicine
      Sŏul, Seoul, South Korea
  • 2011–2013
    • Inha University
      • • Department of Plastic Surgery
      • • Department of Materials Science and Engineering
      Seoul, Seoul, South Korea
  • 2010–2013
    • Kyungpook National University
      • • Department of Chemistry
      • • School of Medicine
      Daikyū, Daegu, South Korea
    • University of Minnesota Duluth
      Duluth, Minnesota, United States
  • 2004–2013
    • Inha University Hospital
      Sinhyeon, South Gyeongsang, South Korea
  • 2011–2012
    • Chonbuk National University Hospital
      Sŏul, Seoul, South Korea
  • 2010–2012
    • Kyung Hee University Medical Center
      Sŏul, Seoul, South Korea
  • 2009–2012
    • Asan Medical Center
      Sŏul, Seoul, South Korea
  • 2007–2012
    • Yonsei University
      • • College of Life Science and Biotechnology
      • • Department of Mathematics
      • • Department of Biology
      Sŏul, Seoul, South Korea
  • 2001–2011
    • Seoul National University
      • • Department of Agricultural Biotechnology
      • • Department of Health Policy and Management
      • • Department of Materials Science and Engineering
      • • Department of Pathology
      • • Division of Chemistry and Molecular Engineering
      Sŏul, Seoul, South Korea
  • 2008–2010
    • Ulsan University Hospital
      Urusan, Ulsan, South Korea
  • 2008–2009
    • Seoul National University Hospital
      • Department of Internal Medicine
      Sŏul, Seoul, South Korea
  • 2007–2008
    • Sungkyunkwan University
      • Department of Nuclear Medicine
      Sŏul, Seoul, South Korea
  • 2003–2005
    • Kyungsung University
      • Department of Food Science and Biotechnology
      Tsau-liang-hai, Busan, South Korea
    • Seegene Institute of Life Sciences
      Sŏul, Seoul, South Korea