D A Lewis

Publications (1)2.59 Total impact

• Source

  Available from: Vivek Naranbhai

  Article: Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.

  H A Hong, A S Loubser, D de Assis Rosa, V Naranbhai, W Carr, M Paximadis, D A Lewis, C T Tiemessen, C M Gray
  [show abstract] [hide abstract]
  ABSTRACT: The effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa.
  Tissue Antigens 09/2011; 78(3):185-94. · 2.59 Impact Factor