Gudrun Abel

Publications (3)6.46 Total impact

• Article: Isolation and characterisation of a cysteine protease (phytolacain G), from Phytolacca americana roots.

  Ursula Sussner, Gudrun Abel, Ralf Schulte, Wolfgang Kreis
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  ABSTRACT: Protein extracts obtained from dried and fresh roots of Phytolacca americana L. (Phytolaccaceae) were examined in order to identify and characterise individual proteins. The extracts were compared with a commercial pokeweed mitogen standard using SDS polyacrylamide gel electrophoresis (SDS-PAGE). A dominant protein, present in both the extracts and the pokeweed mitogen standard, was isolated by subsequent ammonium sulphate fractionation, anion exchange chromatography, gel filtration and SDS-PAGE. In this way it was purified 140-fold with about 20 % yield and 70-fold with about 13 % yield from dried and fresh roots, respectively. Its molecular mass as determined by gel filtration and SDS-PAGE was estimated to be about 25 kDa. Subsequent isoelectric focussing revealed one single protein band at pH 6.0. LysC digestion of the 25 kDa protein yielded several peptides which were subjected to micro-sequencing. Comparison with published sequences revealed that the protein isolated was phytolacain G, a cysteine protease previously isolated from unripe fruits of P. americana L. The enzyme showed a high affinity towards the oxidised insulin B-chain and was completely inhibited by trans-epoxysuccinyl- L-leucylamido(4-guanidino)-butane (E64). The purified phytolacain G showed "lectin-like" activities such as haemagglutination and mitogenic effects towards human lymphocytes.
  Planta Medica 11/2004; 70(10):942-7. · 2.15 Impact Factor
• Article: Isopimpinellin - a furocoumarin from Heracleum sphondylium with chromosome damaging activity.

  Gudrun Abel, Clemens Erdelmeier, Beat Meier, Otto Sticher
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  ABSTRACT: Effects on chromosomes were studied in human lymphocytes IN VITRO as a result of treatment by isopimpinellin plus UV-A. Isopimpinellin was isolated from HERACLEUM SPHONDYLIUM with centrifugal accelerated layer chromatography (CLC). No induction of sister chromatid exchanges was found. Isopimpinellin plus UV-A exhibited, however, a weak clastogenic potency, which was expressed by an enhancement of the gap and break rates by a factor of 2 to 3. The relation between clastogenic and phototoxic activity of furocoumarins is critically discussed.
  Planta Medica 07/1985; 51(3):250-252. · 2.15 Impact Factor
• Article: [Vergleich der genotoxischen Aktivität von Aristolochiasäure in verschiedenen Testsystemen.]

  Oskar Schimmer, Gudrun Abel, Günter Robisch, Waltraud Göggelmann
  Planta Medica 07/1982; 45(7):136. · 2.15 Impact Factor