Barada Kanta Mishra

Institute of Minerals and Materials Technology, Bhubaneswar, Orissa, India

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Publications (40)54.25 Total impact

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    ABSTRACT: Biological sequestration of cadmium (Cd) and retention of adsorbed cadmium as cadmium sulphide (CdS) nanoparticles inside the cell by a lipid-producing green algae Scenedesmus-24 is reported. The microalga was able to grow in the growth media containing 30 mg L−1 of cadmium without any growth inhibition. Adsorption of Cd(II) was dependent on the pH of the medium, initial concentration of cadmium, density of algal biomass (biosorbent dose), and contact time. The adsorption follows Langmuir isotherm pattern with an estimated maximum cadmium adsorption capacity at 50 mg g−1. The kinetics of adsorption followed Lagergren’s pseudo-second-order model. FTIR analysis revealed the presence of different functional groups on the algal biomass which may be responsible for adsorption of Cd(II). After adsorption, the bound metal ions were retained in the microalgal biomass as CdS nanoparticles. Presence of CdS nanoparticle was confirmed by XRD and TEM analysis. The results of the present study conclusively demonstrate that the microalga Scenedesmus-24 may be a promising candidate for sequestration of cadmium from cadmium polluted water and also its recovery as precious CdS nanoparticles.
    Journal of Applied Phycology 12/2014; · 2.33 Impact Factor
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    ABSTRACT: This study illustrates a density functional theory (DFT) based approach to design the amine collectors with regards to their interaction with the quartz surface. Mechanism of interaction of primary aliphatic amines and their ether and ester derivatives with the quartz surface has been established. Hydrogen bond geometries and adsorption energies as calculated using DFT reveal that ester amines are stronger surfactants compared to the ether amines and primary alkyl amines. Simulation studies carried out for the different amines with number of carbon atoms varying from 10 to 18 suggest that increasing the number of carbon atoms in the alkyl chain enhances the interaction, but not beyond C14 for primary amines and C16 for its ether and ester derivatives. Presence of both ether and ester group in the alkyl chain facilitates its interaction strength. Adsorption strength of C12 amine containing both ether and ester amine is almost equivalent to that of C16 ester amine.
    Minerals Engineering 12/2014; 69:57-64. · 1.21 Impact Factor
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    ABSTRACT: Polarization studies have indicated occurrence of three reactions on the cathode surface during precipitation of nickel hydroxide. Out of the three reactions, the nitrite forming reaction followed by conversion to ammonium ion might be the reaction path. The reaction scheme is well supported by the trends in variations in open-circuit potential. Detail prolonged galvanostatic precipitation studies have shown that (OH)−/Ni2+ ratio plays an important role in determining the phase of the nickel hydroxide. A ratio of (OH)−/Ni2+ higher than 6 with sufficient nickel ion in bath ensures β-nickel hydroxide with bigger particles and lesser H2O molecules within. Maintaining these ratios at lesser levels forms α-nickel hydroxide with finer size and more H2O molecules within. The mechanism of these products formations has been explained in this paper. It is also observed that finer size with higher surface area and higher H2O molecules in crystal structure results in higher discharge capacity.
    Journal of Power Sources 10/2014; · 5.26 Impact Factor
  • Korean Journal of Chemical Engineering 10/2014; · 1.06 Impact Factor
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    ABSTRACT: Two different microbial biosurfactants S9BS and CHBS were isolated from Lysinibacillus fusiformis S9 and Bacillus tequilensis CH. Cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (HEK-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT) assay and morphological changes were observed under inverted microscope. The biosurfactants exhibited positive cytotoxicity effect on HEK-293 cell line. It was found that LC50 of S9BS and CHBS were75 μg ml-1 and 100 μg ml-1 respectively. Further cell cycle and apoptosis analysis of biosurfactant treated HEK-293 cell line was done by FACS. In this study, cytotoxicity effect of glycolipid biosurfactant against HEK-293 cell lines is reported for the first time. Mechanism towards membrane permeabilization of biosurfactant treated cancer cell was hypothesized to be most likely by means of incorporation of its lipid moiety into the plasma membrane leading to formation of pores. Hence, these microbial biosurfactants can prove to be significant biomolecule for cancer treatment.
    Applied Biochemistry and Biotechnology 08/2014; · 1.89 Impact Factor
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    ABSTRACT: Light induced biosynthesis of polycrystalline silver nanoparticles (SNPs) using the aqueous extract of a diatom Amphora- 46 was studied. Rapid formation of stable SNPs were observed only on exposure of the reaction mixture to light. Strong surface plasmon resonance at 415 nm due to SNP formation was confirmed by spectroscopic analysis. TEM analysis confirmed the formation of polycrystalline spherical SNPs of an average size of 20-25 nm which was further corroborated by XRD, EDAX and SAED results. Compositional analysis using EDAX showed strong characteristic signal for silver. The XRD spectra shows four intense diffraction peaks at 38.48°, 44°, 64.74°, and 77.4° which correspond well to (111), (200), (220), and (311) plane of (fcc) polycrystalline SNP and the intensity of peak at (111) plane is more than the other peaks, suggesting that this plane is the predominant one. Both XRD and SAED result clearly indicated that the SNPs were polycrystalline in nature and were of high purity. The bio-molecule responsible for the reduction of silver ion was identified to be a photosynthesis pigment fucoxanthin, which is light sensitive and acts as a reducing agent. Furthermore, the synthesized SNPs possess significant antimicrobial activity against gram positive and gram negative bacteria. This study demonstrates for the first time, the involvement of photosynthetic pigment fucoxanthine isolated from Amphora- 46 in silver nanoparticle formation through a light dependent reaction.
    Journal of Saudi Chemical Society 07/2014; · 1.29 Impact Factor
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    ABSTRACT: Direct reduced iron (DRI) is obtained from iron ore by reduction using microwave assisted low temperature hydrogen plasma. The process includes steps of: (a) loading iron ore onto a sample holder (b) placing the sample holder inside a chamber followed by evacuating the chamber; (c) introducing hydrogen gas in the chamber at flow rate ranging between 100-500 standard cubic centimeters (sccm) followed by heating the sample holder and the iron ore at a temperature ranging between 300-800° C., a pressure ranging between 20-100 torr and a microwave power ranging between 500-1500 W to obtain direct reduced iron; and (d) cooling the direct reduced iron obtained in step (c) by flowing hydrogen at flow rate of about 300 sccm.
    Ref. No: US8728195 B2, Year: 05/2014
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    ABSTRACT: Bionanotechnology has revolutionized the nano material synthesis by providing a green synthetic platform using its biological systems. Among the biological systems microalgae has tremendous potential to uptake metal ions and produce nanoparticle by detoxification process. The present study explores the intracellular and extracellular biogenic synthesis of silver nanoparticles (SNPs) using a unicellular green microalga Scenedesmus. Biosynthesized SNPs were characterized by AAS, UV-Vis spectroscopy, TEM, XRD, FTIR, DLS, TGA studies and finally checked for antibacterial activity. Intracellular nanoparticle biosynthesis is initiated by high rate of Ag(+) ion accumulation in the microalgal biomass and subsequent formation of spherical crystalline SNPS (of average size of 15-20 nm) due to biochemical reduction of Ag(+) ions. The synthesized nanoparticles were intracellular as confirmed by the UV-Vis spectra of experimental medium. Furthermore, extracellular synthesis using boiled extract shows formation of well scattered, highly stable, spherical SNPs with average size of 5-10 nm. Size and morphology of the nanoparticle was confirmed by TEM. Crystalline nature of the SNPs was evident from the diffraction peaks of XRD and bright circular ring pattern of SAED. FTIR and UV-Vis spectra showed that the biomolecules, proteins and peptides, are mainly responsible for formation and stabilization of SNPs. Further, the synthesized nanoparticles exhibited high antimicrobial activity against pathogenic gram negative and gram positive bacteria. Use of such a microalgal system provides a simple, cost effective alternative template for biosynthesis of nanomaterials in large scale system with a great use in biomedical application.
    Journal of Microbiology and Biotechnology 01/2014; · 1.40 Impact Factor
  • Industrial and Environmental Biotechnology, 01/2014: chapter 6;
  • Korean Journal of Chemical Engineering 01/2014; · 1.06 Impact Factor
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    ABSTRACT: A biogenic route was adopted towards the synthesis of gold nanoparticles using the extract of a novel strain, Talaromyces flavus . Reduction of chloroauric acid by the fungal extract resulted in the production of gold nanoparticle, which was further confirmed by the concordant results obtained from UV–visible spectroscopy, energy dispersive spectroscopy (EDS), and dynamic light scattering (DLS) analysis. Morphology and the crystal nature of the synthesized nanoparticles were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD) and selected area electron diffraction (SAED). A direct correlation was observed between nanoparticle formation and the concentration of reducing agent present in the fungal extract. The time-dependent kinetic study revealed that the bioreduction process follows an autocatalytic reaction. Crystalline, irregular, and mostly flowershaped gold nanoparticles with a mean hydrodynamic radius of 38.54±10.34 nm were obtained. pH played a significant role on production of mono-dispersed nanoparticle. FTIR analysis partially deciphered the involvement of –NH2, −SH, and –CO groups as the probable molecules in the bio-reduction and stabilization process. Compared to the conventional methods, a time-resolved, green, and economically viable method for floral-shaped nanoparticle synthesis was developed.
    Annals of Microbiology 11/2013; · 1.55 Impact Factor
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    ABSTRACT: Microorganisms are a scientific asset as they remarkably orchestrate life on earth. Microorganisms play a significant role in the various cycles of terrestrial and aquatic ecosystems. Specific microbes from the ancient Precambrian age have key impact in the biogeochemical cycles of carbon and iron due to their immense metabolic versatility. They possess the unique ability to utilize a wide range of soluble electron acceptors such as nitrate, nitrite, sulphur, trimethylamine N-oxide, dimethyl sulfoxide as well as insoluble oxidized metals such as iron and manganese, various radionuclides like uranium, plutonium, etc. Of these, the dissimilatory iron reducing bacteria (DIRB) has found widespread attention due to their ability to reduce Fe (III) as sole electron acceptor coupled to the oxidation of organic carbon source (electron donor) under anerobic/facultative anaerobic conditions. This ability of DIRB was exploited in our study to extract nickel and cobalt from chromite overburden (COB). Nickel is engrained within the goethite phase of COB which restrains its release, thus rendering conventional leaching methods inefficient. A phase change of this ore to its reduced form of hematite and magnetite can loosen the matrix, enhancing the extraction efficiency. COB reduction through thermal activation by roasting at high temperatures, through reduction-magnetic separation process with activated carbon powder as the reductant , solid-state deoxidisation method utilise an energy intensive approach makes it more uneconomic and less eco-friendly. Hence, nickel extraction using microbes has gained interest in the recent past. Several studies have focused on bioleaching of COB using fungi and chemolithotrophs. These microbial processes have exhibited issues of low extraction efficiency, laborious downstream processing due to substantial fungal biomass, and tedious requirement of pH maintenance in case of chemolithotrophs. Here, we subjected the COB to microbial reduction using DIRB followed by acid leaching for enhanced nickel-cobalt recovery. Various DIRB consortia were isolated from different marshy areas and screened the consortia capable of efficiently reducing Fe (III) of Chromite overburden (COB) under facultative anaerobic condition. DIRB was found to liberate the goethite-associated nickel as a result of the phase change in ore during its growth. The phase change was confirmed by XRD analysis, wherein the goethite and hematite peaks were observed in original COB while DIRB-treated COB showed a nickel oxide and magnetite peaks in addition to goethite and hematite. The phase change is pertained to the microbial reduction of Fe (III) to Fe (II). Morphological study using FESEM showed a variance from needle shaped goethite in original ore to granular magnetite in treated ore. About 27% nickel was obtained on bio-reduction of COB which was enhanced by 43% on treating with 8M sulphuric acid, resulting in 70% Ni recovery.
    INTERNATIONAL CONFERENCE ON CONSERVING BIODIVERSITY FOR SUSTAINABLE DEVELOPMENT((INCCBSD 2013), NATIONAL INSTITUTE OF TECHNOLOGY,Rourkela; 08/2013
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    ABSTRACT: Biosurfactants are amphiphilic molecules having hydrophobic and hydrophilic moieties produced by various microorganisms. These molecules trigger the reduction of surface tension or interfacial tension in liquids. A biosurfactant-producing halophile was isolated from Lake Chilika, a brackish water lake of Odisha, India (19°41′39″N, 85°18′24″E). The halophile was identified as Bacillus tequilensis CH by biochemical tests and 16S rRNA gene sequencing and assigned accession no. KC851857 by GenBank. The biosurfactant produced by B. tequilensis CH was partially characterized as a lipopeptide using thin-layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance techniques. The minimum effective concentration of a biosurfactant for inhibition of pathogenic biofilm (Escherichia coli and Streptococcus mutans) on hydrophilic and hydrophobic surfaces was found to be 50 μg ml−1. This finding has potential for a variety of applications.
    Applied Biochemistry and Biotechnology 08/2013; · 1.89 Impact Factor
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    ABSTRACT: The effect of an adapted Dissimilatory Iron Reducing bacterial consortium (DIRB) towards bio-reduction of Sukinda Chromite overburden (COB) with enhanced recovery of nickel and cobalt is being reported for the first time. The remarkable ability of DIRB to utilize Fe (III) as terminal electron acceptor reducing it to Fe (II) proved beneficial for treatment of COB as compared to previous reports for nickel leaching. XRD studies showed goethite as the major iron-bearing phase in COB. Under facultative anaerobic conditions, goethite was reduced to hematite and magnetite with the exposure of nickel oxide. FESEM studies showed DIRB to be associated with COB through biofilm formation with secondary mineral precipitates of magnetite deposited as tiny globular clusters on the extra polymeric substances. The morphological and mineralogical changes in COB, post DIRB application, yielded a maximum of 68.5% Nickel and 80.98% Cobalt in 10 days using 8M H2SO4.
    Bioresource Technology 07/2013; · 5.04 Impact Factor
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    ABSTRACT: Recovery of metal value, especially from low-grade ores and overburden minerals using acidophilic bacteria through the process of bioleaching is an environmentally benign and commercially scalable biotechnology. In recent years, while the 'OMICS' landscape has been witnessing extensive application of computational tools to understand and interpret global biological sequence data, a dedicated bioinformatic server for analysis of bacterial information in the context of its bioleaching ability is not available. We have developed an on-line Bacterial Bioleaching Protein Finder (BBProF) System, which rapidly identifies novel proteins involved in a bacterial bioleaching process and also performs phylogenetic analysis of 16S rRNA genes. BBProF uses the features of Asynchronous Java Script and XML (AJAX) to provide an efficient and fast user experience with minimal requirement of network bandwidth. In the input module the server accepts any bacterial or archaeal complete genome sequence in RAW format and provides a list of proteins involved in the microbial leaching process. BBProF web server is integrated with the European Bioinformatics Institute (EBI) web services such as BLAST for homology search and InterProScan for functional characterization of output protein sequences. Studying evolutionary relationship of bacterial strains of interest using Muscle and ClustalW2 phylogeny web services from EBI is another key feature of our server, where 16S rRNA gene sequences are considered as input through a JQUERY interface along with the sequences present in the BBProF database library. Complete genome sequences of 24 bioleaching microorganism characterized by genomic and physiological study in the laboratory and their respective 16S rRNA gene sequences were stored in the database of the BBProF library. To our knowledge BBProF is the first integrated bioinformatic web server that demonstrates its utility in identifying potential bioleaching bacteria. We hope that the server will facilitate on-going comparative genomic studies of bioleaching microorganisms and also assist in identification and design of novel microbial consortia that are optimally efficient bioleaching agents.
    Geomicrobiology 07/2013; · 1.61 Impact Factor
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    ABSTRACT: Acetyl-CoA carboxylase (ACCase), a biotin-dependent enzyme that catalyses the first committed step of fatty acid biosynthesis, is considered as a potential target for improving lipid accumulation in oleaginous feedstocks, including microalgae. ACCase is composed of three distinct conserved domains, and understanding the structural details of each catalytic domain assumes great significance to gain insights into the molecular basis of the complex formation and mechanism of biotin transport. In the absence of a crystal structure for any single heteromeric ACCase till date, here we report the first heteromeric association model of ACCase from an oleaginous green microalga, Chlorella variabilis, using a combination of homology modelling, docking and molecular dynamic simulations. The binding site of the docked biotin carboxylase (BC) and carboxyltransferase (CT) were predicted to be contiguous but distinct in biotin carboxyl carrier protein (BCCP) molecule. Simulation studies revealed considerable flexibility for the BC and CT domains in the BCCP-bound forms, thus indicating the adaptive behaviour of BCCP. Further, principal component analysis revealed that in the presence of BCCP, the BC and CT domains exhibited an open-state conformation via the outward clockwise rotation of the binding helices. These conformational changes might be responsible for binding of BCCP domain and its translocation to the respective active sites. Various rearrangements of inter-domain hydrogen bonds (H-bonds) contributed to conformational changes in the structures. H-bond interactions between the interacting residue pairs involving Glu201BCCP/Arg255BC and Asp224BCCP/Gln228CT were found to be essential for the intermolecular assembly. The present findings are consistent with previous biochemical studies.
    Applied biochemistry and biotechnology 05/2013; · 1.94 Impact Factor
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    ABSTRACT: Optimization studies of plasma smelting of red mud were carried out. Reduction of the dried red mud fines was done in an extended arc plasma reactor to recover the pig iron. Lime grit and low ash metallurgical (LAM) coke were used as the flux and reductant, respectively. 2-level factorial design was used to study the influence of all parameters on the responses. Response surface modeling was done with the data obtained from statistically designed experiments. Metal recovery at optimum parameters was found to be 79.52%.
    Plasma Science and Technology 05/2013; 15(5):459. · 0.51 Impact Factor
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    ABSTRACT: This article depicts the solid particle erosion response of plasma-sprayed composite coatings using an industrial waste product (i.e., copper slag) and aluminum. The influence of five operating parameters—that is, impact velocity, erodent size, erodent temperature, impingement angle, and aluminum content of feedstock with four different levels each—on performance output (i.e., erosion rate) are studied using Taguchi's L16 orthogonal array design. Out of the five parameters, impact velocity has been found to be most influential factor on the erosion wear rate of coated samples. Maximum erosion takes place at an impingement angle of 60°, showing the semiductile response of the coating to solid particle erosion. In addition, a multilinear mathematical equation is proposed in order to predict the wear rate. The percentage of error between experimental data and predicted data was small, with a very high correlation coefficient (r2) of 0.997 showing the correctness of the mathematical equation used.
    Tribology Transactions 03/2013; 56(2). · 0.91 Impact Factor
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    ABSTRACT: This paper presents the application of the Taguchi experimental design in developing nanostructured yittria stabilized zirconia (YSZ) coatings by plasma spraying process. This paper depicts dependence of adhesion strength of as-sprayed nanostructured YSZ coatings on various process parameters, and effect of those process parameters on performance output has been studied using Taguchi's L16 orthogonal array design. Particle velocities prior to impacting the substrate, stand-off-distance, and particle temperature are found to be the most significant parameter affecting the bond strength. To achieve retention of nanostructure, molten state of nanoagglomerates (temperature and velocity) has been monitored using particle diagnostics tool. Maximum adhesion strength of 40.56 MPa has been experimentally found out by selecting optimum levels of selected factors. The enhanced bond strength of nano-YSZ coating may be attributed to higher interfacial toughness due to cracks being interrupted by adherent nanozones.
    The Scientific World Journal 01/2013; 2013:527491. · 1.73 Impact Factor