Publications (14)18.75 Total impact
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Article: Butyrylcholinesterase (BChE) Activity Is Associated with the Risk of Preeclampsia: Influence on Lipid and Lipoprotein Metabolism and Oxidative Stress.
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ABSTRACT: Abstract Objective: To determine the butyrylcholinesterase (BChE) activity and phenotypes in preeclampsia and its possible association with lipid and lipoprotein metabolism and oxidative stress in preeclamptic women. Methods: In a case-control study 101 pregnant women with normal pregnancy and 198 women with preeclampsia from Western Iran were studied. The serum BChE activity and phenotypes were measured using spectrophotometric method. The apolipoprotein E (APOE) genotypes were identified using PCR-RFLP. The serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined by HPLC and commercial kits, respectively. Results: The BChE activity and the frequency of non UU (non usual) BChE phenotype in preeclamptic women were significantly lower and higher, respectively compared to controls. There was a higher BChE activity in the presence of APOE ε3ε4 compared to ε3ε3 genotype in preeclamptic women. In addition, there were significant positive correlations between BChE activity and the levels of LDL-C, HDL-C, total cholesterol (TC) and TAC. However, there was a negative but significant correlation between BChE activity and MDA level. Conclusions: Our study for the first time indicated that BChE activity might be involved in the pathogenesis of preeclampsia through influence on lipid and lipoprotein metabolism and oxidative stress.The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 05/2013; · 1.36 Impact Factor -
Article: MMP-9 (-1562 C:T) polymorphism as a biomarker of susceptibility to severe pre-eclampsia.
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ABSTRACT: Aim: To investigate the role of MMP-9 (-1562 C:T) polymorphism as a biomarker for susceptibility to pre-eclampsia. Patients & methods: MMP-9 variants were detected in 160 women with mild and severe pre-eclampsia and 112 healthy pregnant women. Results: A significantly higher frequency of MMP-9 CT genotype was observed in both mild and severe pre-eclamptic women compared with controls. In the presence of CT + TT genotype the risk of severe pre-eclampsia increased 2.37-fold (p = 0.02). In women with early-onset pre-eclampsia the frequency of MMP-9 CT + TT genotype was significantly higher (p = 0.045) compared with those with late-onset pre-eclampsia. Conclusion: The MMP-9 variant could be a useful biomarker of susceptibility to severe pre-eclampsia and early-onset severe pre-eclampsia.Biomarkers in Medicine 02/2013; 7(1):93-8. · 0.86 Impact Factor -
Article: Synergistic effects of angiotensinogen -217 G->A and T704C (M235T) variants on the risk of severe preeclampsia.
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ABSTRACT: BACKGROUND: The rate-limiting step of the renin-angiotensin system is the enzymatic cleavage of angiotensinogen (AGT) by renin. The aims of the present study were to investigate the association between AGT T704C (M235T) and -217 G→A polymorphisms with the risk of preeclampsia and synergistic effects of both polymorphisms on the susceptibility to preeclampsia. METHODS: We studied AGT variants in 170 women with preeclampsia, including 84 women with mild and 86 women with severe forms of preeclampsia, and 100 age and parity matched controls. RESULTS: There was a trend towards increased risk of severe preeclampsia in the presence of -217 AA (odds ratio (OR)=1.5, 95% confidence interval (CI)= 0.38-5.84, p=0.57) and TC+CC genotypes (OR=1.32, 95% CI= 0.67-2.58, p=0.42). However, the interaction of both alleles of -217A and 704C highly increased the risk of severe preeclampsia, by 2.23-fold, although this did not reach statistical significance. The frequency of the CC genotype of the T704C polymorphism in early-onset preeclampsia tended to be higher (35%) compared with that in patients with late-onset preeclampsia (21.7%). CONCLUSIONS: The present study demonstrates that both variants of AGT -217 G→A and T704C might work in synergism to influence the risk of severe preeclampsia, which needs to be confirmed in studies with larger sample size.Journal of Renin-Angiotensin-Aldosterone System 11/2012; · 2.44 Impact Factor -
Article: MTHFR C677T and eNOS G894T Variants in Preeclamptic Women: Contribution to Lipid Peroxidation and Oxidative Stress.
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ABSTRACT: OBJECTIVES: We aimed to investigate the association between methylenetetrahydrofolate reductase (MTHFR) C677T and endothelial nitric oxide synthase (eNOS) G894T polymorphisms with lipid peroxidation, total antioxidant capacity (TAC) and the risk of preeclampsia in preeclamptic women. DESIGN AND METHODS: We screened a sample of 198 unrelated women with mild and severe forms of preeclampsia and 101 unrelated women with normal pregnancy with the eNOS and MTHFR variants using PCR-RFLP method. Also, the serum malondialdehyde (MDA) and TAC levels were determined using HPLC and commercial kits, respectively. RESULTS: The frequency of combined genotypes of MTHFR CT and TT (CT+TT) and T allele tended to be higher in severe preeclamptic women compared to controls. There was no significant difference for eNOS G894T genotype and allele frequencies between patients and controls. A significantly higher level of triglycerides was observed in the presence of combined genotypes of MTHFR CT and TT and also eNOS GT and TT (GT+TT) in preeclamptic women compared to controls with the same genotype. Also, the presence of MTHFR TT genotype in severe preeclamptic women was significantly associated with the increased serum MDA level compared to CC genotype. In severe preeclamptic women the presence of CT and combined genotypes of CT and TT was significantly associated with the decreased TAC level compared to CC genotype. Also, a higher MDA level was observed in mild preeclamptic women with eNOS TT genotype compared to those with GG genotype but the difference was not significant. CONCLUSION: Present study indicates that MTHFR C677T polymorphism through affecting on TG level, lipid peroxidation and oxidative stress might be involved in the pathogenesis of severe preeclampsia.Clinical biochemistry 10/2012; · 2.02 Impact Factor -
Article: Preeclampsia and angiotensin converting enzyme (ACE) I/D and angiotensin II type-1 receptor (AT1R) A1166C polymorphisms: association with ACE I/D polymorphism.
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ABSTRACT: BACKGROUND: The aim of the present study was to investigate the association between angiotensin converting enzyme (ACE) insertion/deletion (I/D) and angiotensin II type-1 receptor (AT1R) A1166C polymorphisms with the risk of preeclampsia and lipid peroxidation in preeclamptic women from Western Iran. METHODS: One hundred and ninety-eight preeclamptic women (128 women with mild and 70 with severe forms) and 100 age- and parity-matched controls were enrolled in this case-control study. RESULTS: The presence of D allele of ACE was associated with a 1.8-fold increased risk of preeclampsia (p=0.002) in total preeclamptic patients. The frequency of AT1R AC+CC genotypes was higher in mild preeclamptic women (32%) compared to controls (27.2%) (p>0.05). In mild preeclamptic women with ID genotype, the level of total antioxidant capacity (TAC) was significantly decreased compared to those with II genotype. Also, there was a trend toward increasing malondialdehyde (MDA) and decreasing TAC levels in mild and severe preeclamptic women with AT1R AA through CC genotypes. CONCLUSIONS: Our study indicates that lipid peroxidation and oxidative stress are involved in the development of preeclampsia that might be influenced by polymorphism in the renin-angiotensin-aldosterone system genes.Journal of Renin-Angiotensin-Aldosterone System 06/2012; · 2.44 Impact Factor -
Article: Apolipoprotein E Genotypes, Lipid Peroxidation, and Antioxidant Status among Mild and Severe Preeclamptic Women from Western Iran: Protective Role of Apolipoprotein ϵ2 Allele in Severe Preeclampsia.
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ABSTRACT: Objective. The aim of this study was to examine the association of apolipoprotein E (APOE) genotypes and oxidative stress with the risk of mild and severe preeclampsia. Methods. In a case-control study, 198 women with preeclampsia including 128 women with mild and 70 women with severe preeclampsia and 101 control pregnant women from Western Iran were studied. The APOE genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The serum level of malondialdehyde (MDA) and total antioxidant capacity (TAC) were determined using high-performance liquid chromatography and commercial kits, respectively. Results. The frequency of APOE ϵ2 allele in severe preeclamptic women (2.1%) was significantly (p = 0.008) lower than that in controls (9.4%). The presence of APOE ϵ2 allele was associated with around five times decreased risk of severe preeclampsia [OR = 0.21 (95% CI = 0.6-0.73, p = 0.014)]. A significantly higher serum level of MDA was observed in women with severe (10.87 ± 4.61 μM) and mild (9.81 ± 3.67 μM) preeclampsia compared with that in controls. A trend toward decrease serum level of MDA was observed according to the APOE alleles as ϵ2 < ϵ3 < ϵ4 (9.23, 10.22, and 10.43 μM, respectively). In preeclamptic women, an inverse correlation was detected between serum levels of MDA and HDL-C (r = -0.16, p = 0.029). However, there was a direct correlation between serum level of MDA with diastolic blood pressure (r = 0.15, p = 0.037). Conclusion. Our study in a population with Kurdish ethnic background indicates a protective role for APOE ϵ2 allele against severe preeclampsia that might be through high antioxidant capacity of this allele.Hypertension in Pregnancy 06/2012; 31(4):405-18. · 1.69 Impact Factor -
Article: Concomitant presence of endothelial nitric oxide 894T and angiotensin II-converting enzyme D alleles are associated with diabetic nephropathy in a Kurdish population from Western Iran.
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ABSTRACT: The present study investigated the influence of insertion (I)/deletion (D) polymorphism of the angiotensin II-converting enzyme (ACE) gene in combination with endothelial nitric oxide (eNOS) G894T polymorphism on the predisposition to diabetic nephropathy (DN). Using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) method, the ACE and eNOS polymorphisms were genotyped in 72 microalbuminuric, 68 macroalbuminuric and 72 normoalbuinuric type 2 diabetes mellitus (T2DM) patients from Western Iran. The presence of eNOS T or ACE D allele was not associated with increased risk of macroalbuminuria (odds ratio (OR) = 1.36, P = 0.27 and OR = 1.6, P = 0.062, respectively). However, in the presence of both alleles there was a trend towards increased risk of macroalbuminuria (fivefold, P = 0.05). Our study indicates that the concomitant presence of both ACE D and eNOS T alleles tends to be associated with an elevation risk of macroalbuminuria compared with the presence of each polymorphism alone. This risk could be attributed to the increasing activity of ACE and angiotensin II level in the presence of D allele and decreasing NO production in the presence of T allele accelerating diabetic nephropathy.Nephrology 02/2012; 17(2):175-81. · 1.31 Impact Factor -
Dataset: Association of Factor V Leiden Mutation with Pediatric Acute lymphoblastic Leukemia in Kermanshah Province
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Dataset: Interaction of thymidylate synthase polymorphism with MTHFR variants modify the risk of childhood acute lymphoblastic leukemia
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ABSTRACT: A genetic susceptibility to acute lymphoblastic leukemia (ALL) has been suggested. The present study was conducted to examine the influence of interaction between methylenetetrahydrofolate reductase (MTHFR) variants and thymidylate synthase (TS) 28-base pair (bp) repeat polymorphism on the risk of pediatric ALL. Sixty eight ALL children with the mean age of 8.19±4.0 years and 70 age-and sex-matched healthy children were studied for TS 28-bp repeat and MTHFR C677T and A1298C variants using PCR and PCR-RFLP, respectively. The presence of TS 2R allele had a protective effect on the risk of ALL that did not reach a statistical significance [OR=0.5 (95% CI 0.19-1.24, p=0.13)]. Also, the effect of MTHFR 677T allele on the risk of ALL was similar to TS 2R allele [OR=0.5 (95% CI 0.18-1.4, p=0.19)]. However, there was a trend toward increased risk of ALL in the presence of both TS 2R and MTHFR 677T alleles [OR=1.92 (95% CI 0.7-5.3, p=0.2)]. In contrast, the overall distribution of interaction between TS 2R and MTHFR 1298C alleles in ALL patients compared to controls was not significant. Our results suggest that the interaction between polymorphisms of different genes instead of the one polymorphism in a single gene might determine the susceptibility to pediatric ALL. -
Article: Strong interaction between T allele of endothelial nitric oxide synthase with B1 allele of cholesteryl ester transfer protein TaqIB highly elevates the risk of coronary artery disease and type 2 diabetes mellitus.
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ABSTRACT: The present study was conducted to investigate the possible outcome of interaction between endothelial nitric oxide (NOS3) G894T and cholesteryl ester transfer TaqIB variants on the risk of coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM). The sample included a total of 207 CAD patients (102 CAD patients with T2DM and 105 CAD patients without T2DM). There were also 101 patients with T2DM and 92 age- and sex-matched healthy individuals as controls. All study participants were from Western Iran. The sample was genotyped by polymerase chain reaction-restriction fragment length polymorphism. The presence of NOS3 T allele was not associated with the risk of CAD or T2DM, and the CETP B1 allele was only significantly associated with the increased risk of CAD in total CAD patients (odds ratio (OR) = 5.1, p = 0.019). However, the concomitant presence of both CETP B1 and NOS3 T alleles significantly increased the risk of CAD in total CAD patients (OR = 18.1, p < 0.001), in CAD patients without T2DM (OR = 27.1, p = 0.03), and in CAD patients with T2DM (OR = 13.5, p = 0.002). Also, the presence of both alleles increased the risk of T2DM (OR = 12, p = 0.004). Our findings, for the first time, indicate that NOS3 T allele strongly interacts with CETP B1 allele to augment the risk of CAD and T2DM in the population of Western Iran.Human genomics 01/2012; 6(1):20. -
Article: Thrombophilic mutations and susceptibility to preeclampsia in Western Iran.
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ABSTRACT: The aim of the present study was to investigate the frequency and the possible association between thrombophilic mutations of factor V Leiden (FVL) and prothrombin G20210A with preeclampsia among Kurdish population of Western Iran. We studied 198 women with preeclampsia including 128 women with mild and 70 women with severe forms and 101 healthy pregnant women with uncomplicated pregnancy. Among cases there were 23 women with early onset preeclampsia and 175 cases with late-onset preeclampsia. The sample was genotyped by polymerase chain reaction-restriction fragment-length polymorphism using Mnl I and Hind III for FVL and prothrombin G20210A, respectively. The frequency of heterozygous FVL mutation was 7.6% among all preeclamptic women (8.6% in mild and 5.7% in severe preeclamptic women) and 7.9% in controls (P > 0.05). However, the prevalence of heterozygous FVL were 10.5 and 3.9% among severe preeclamptic women with early onset and late-onset preeclampsia, respectively (P > 0.05). The prevalence of prothrombin G20210A were 1.6, 2.9, and 3% among women with mild preeclamsia, severe preeclampsia and controls, respectively (P > 0.05). The level of serum triglycerides (TG) was significantly higher among women with preeclampsia compared to healthy pregnant women that was not associated with the two thrombophilic mutations. Our results indicate that neither FVL nor prothrombin G20210A could be a risk factor for preeclampsia in our population. However, high prevalence of FVL in preeclamptic women with early onset compared to those with late-onset preeclampsia may suggest a role for this mutation in predisposition to early onset preeclampsia that need to be confirmed with larger sample size.Journal of Thrombosis and Thrombolysis 11/2011; 33(1):109-15. · 1.48 Impact Factor -
Article: Lack of association between MTHFR C677T and A1298C polymorphisms and risk of childhood acute lymphoblastic leukemia in the Kurdish population from Western Iran.
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ABSTRACT: Polymorphism in genes involved in folate metabolism may influence the susceptibility to acute lymphoblastic leukemia (ALL). The aim of the present study was to determine the role of the two most common polymorphisms of the 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene, MTHFR C677T and A1298C, and their interaction on the susceptibility to ALL. Seventy-two children with ALL and 109 age- and sex-matched healthy children from Western Iran were screened for MTHFR C677T and A1298C variants by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The frequencies of MTHFR 677T and 1298C alleles in patients were 29.9% and 43.1%, respectively, that were higher than those in controls (24.8% and 38.1%, respectively). Logistic regression analysis was performed and its result in the odds ratios (ORs) for possession of either MTHFR 677T or 1298C allele was found to be 1.98 [95% confidence interval (CI) 0.72-5.4, p = 0.18] and 1.48 (95% CI 0.59-3.69, p = 0.4), respectively. Also the concomitant presence of both MTHFR 677T and 1298C alleles was not associated with the risk of ALL [OR = 2.12 (95% CI 0.8-5.7, p = 0.13)]. Our results in a homogenous population with Kurdish ethnic background indicated that neither the MTHFR 677T allele nor the MTHFR 1298C allele is associated with increased risk of ALL.Genetic Testing and Molecular Biomarkers 10/2011; 16(3):198-202. · 1.11 Impact Factor -
Article: Association between cholesteryl ester transfer protein TaqIB variants and risk of coronary artery disease and diabetes mellitus in the population of western Iran.
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ABSTRACT: To shed light on the previously inconsistent results about the association of cholesteryl ester transfer protein TaqIB (CETP TaqIB) variants, high-density lipoprotein cholesterol (HDL-C) levels, and the risk of coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM). To determine the frequency of CETP TaqIB variants and to examine the possible association between CETP TaqIB polymorphism with CAD and T2DM, we studied 207 unrelated patients with CAD, 101 patients with T2DM, and 92 controls. The CETP TaqIB variants were detected by polymerase chain reaction-restriction fragment length polymorphism. Logistic regression analysis indicated that the B1 allele of CETP was significantly associated with increased risk of CAD (odds ratio, OR 1.65 [95% confidence interval, CI 1.2-2.3, p=0.005]) and T2DM (OR 1.7 [95% CI 1.13-2.54, p=0.005]). Adjusted logistic regression analysis for the effects of age, sex, hypertension, diabetes, and hyperlipidemia was performed; and a significant association was found between the B1 allele and risk of CAD (OR 1.9 [95% CI 1-3.6, p=0.049]) in patients with CAD. There were no associations between the CETP alleles and the levels of triglycerides, total cholesterol, low-density lipoprotein cholesterol, and HDL-C in studied groups. The results of the present study revealed that the CETP B1 allele is associated with increased risk of CAD and T2DM independent of plasma HDL-C level in our population.Genetic Testing and Molecular Biomarkers 06/2011; 15(11):813-9. · 1.11 Impact Factor -
Article: Thymidylate synthase and methionine synthase polymorphisms are not associated with susceptibility to childhood acute lymphoblastic leukemia in Kurdish population from Western Iran.
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ABSTRACT: In order to determine the influence of polymorphism in thymidylate synthase (TS 28-bp repeat) and methionine synthase (MS A2756G) genes on the susceptibility to acute lymphoblastic leukemia (ALL), 73 children with ALL and 128 age and sex matched unrelated healthy individuals from the Kermanshah Province of Iran were screened. The genotyping of TS 28-bp repeat and MS A2756G polymorphisms were performed by polymerase chain reaction (PCR) and PCR-RFLP, respectively. The frequency of TS 2R allele in patients and controls were 41.5 and 38%, respectively (Odds ratios (OR) = 1.13, 95%CI 0.73-1.74, P = 0.56). The allelic frequency of G allele of MS was higher (25%) in patients compared with healthy subjects (23%) (OR = 1.09, 95%CI 0.67-1.75, P = 0.71). Considering MS AA and TS 3R3R genotypes as reference indicated that individuals with MS GG + TS 2R2R genotypes have 1.3-fold increase in the risk of ALL (OR = 1.3, 95%CI 0.6-2.7, P = 0.5). Our results showed that neither TS 28-bp repeat nor MS A2756G polymorphisms are risk factors for susceptibility to ALL in Western Iran.Molecular Biology Reports 06/2011; 39(3):2195-200. · 2.93 Impact Factor
Top co-authors
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Institutions
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2011–2012
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Kermanshah University of Medical Sciences
- Medical Biology Research Center (MBRC)
Kermānshāh, Ostan-e Kermanshah, Iran
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