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ABSTRACT: To investigate the role of CD14 promoter - 159 C-> T polymorphism on ratios of serum lipids and its interaction on the ratios with a high-carbohydrate/low-fat (HC/LF) diet in a young and healthy Chinese Han population.
After a washout diet for seven days, fifty six healthy young subjects (22.89 +/- 1.80 years) were given the HC/LF diet for six days. Twelve-hour fasting venous blood samples were collected in the mornings of the first, the eighth and the fourteenth days. The serum lipid profiles and the CD14 -159 C->T polymorphism were analyzed. The ratios of triglyceride/high density lipoprotein-cholesterol (TG/HDL-c), log (TG/HDL-c), total cholesterol/high density lipoprotein-cholesterol (TC/HDL-c) and low density lipoprotein-cholesterol/high density lipoprotein-cholesterol (LDL-c/HDL-c) were calculated. RESULTS :The male carriers of the C allele had significantly higher TG/HDL-c and log (TG/HDL-c) than the female carriers at baseline, after the washout diet and after the HC/LF diet, higher TC/HDL-c at baseline and after the washout diet, and higher LDL-c/HDL-c only after the washout diet. The female subjects with the TT genotype had higher TG/HDL-c and log (TG/HDL-c) than the female carriers of the C allele at baseline, after the washout diet and after the HC/LF diet, higher LDL-c/HDL-c at baseline and after the HC/LF diet, and higher TC/HDL-c only after the washout diet. Compared with that before the HC/LF diet, TC/HDL-c was significantly decreased after the HC/LF diet regardless of gender and the genotype of the CD14 -159 polymorphism. LDL-c/HDL-c was significantly decreased in both the male and female carriers of the C allele. TG/HDL-c and log (TG/HDL-c) were significantly increased only in the female carriers of the C allele.
In the subjects with C allele, the HC/LF diet is a minor factor and its effects on the lipid ratios can be masked by the effects of the C allele at CD14 -159. The interaction between the HC/LF diet and the C allele at CD14 -159 can decrease LDL-c/HDL-c in both males and females and increase TG/ HDL-c and log (TG/HDL-c) in the females.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 05/2012; 43(3):395-400.
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ABSTRACT: To investigate the effects of a high-carbohydrate diet on the lipid and apolipoprotein ratios in healthy young adults with different genotypes of the polymorphism at -75 site in the promoter region of the gene of apolipoprotein AI (APOA1).
Fifty-six subjects aged (22.89 +/- 1.80) years were given a wash-out diet for 7 days, followed by a high-carbohydrate diet for 6 days. The wash-out diet contained 15% protein, 31% fat, and 54% carbohydrate. The high-carbohydrate diet contained 15% protein, 15% fat, and 70% carbohydrate. Twelve-hour fasting serum lipids and apolipoproteins B100 and AI were measured on the mornings of the 1st, the 8th, and the 14th days from the beginning of the wash-out diet. The ratios of triglyceride (TG)/high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC)/HDL-C, low-density lipoprotein cholesterol (LDL-C)/HDL-C, and apolipoprotein B100 (APOB100)/apolipoprotein AI (APOAI) were calculated. The genome DNA was extracted and the polymorphism of APOA1 -75 G/A was determined by polymerase chain reaction followed by restriction fragment length polymorphism assay.
At baseline, the lipid and apolipoprotein ratios showed no significant differences between the GG genotype and the A carriers in males (P > 0.05), whereas the female A carriers had a significantly higher ratio of LDL-C/ HDL-C compared with the female subjects with the GG genotype (P < 0.05). Following the high-carbohydrate diet, significant decreases of TC/HDL-C were found in all the groups, regardless of sex and genotype (P < 0.01). LDL-C/HDL-C experienced significant decreases in both the genotypes in males (P < 0.05), while in females, significant decrease of LDL-C/HDL-C was only observed in A carriers (P < 0.01).
The A allele of the -75 G/A polymorphism in APOA1 may have specific effects on the LDL-C/HDL-C ratio in females.
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 02/2012; 34(1):1-7.
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Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] 02/2012; 40(2):169-72.
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ABSTRACT: To investigate the effects of a low-fat and high-carbohydrate (LF-HC) diet on the physiological and biochemical indexes in healthy youth with different body mass index (BMI).
Seven overweight participants [BMI=(27.82 +/- 1.64) kg/m2] and 49 age-matched controls [BMI = (20.06 +/- 2.41) kg/ m2] were given a washout diet for 7 d, followed by a LF-HC diet for 6 d. The washout diet contained 31.1% fat and 54.1% carbohydrate, and the LF-HC diet contained 14.8% fat and 70.1% carbohydrate of total energy. Anthropometric measurements were conducted on the mornings of the first, eighth and fourteenth days. Serum samples were prepared from twelve-hour fasting venous blood. Biochemical indexes including lipids; glucose and insulin were measured with routine methods. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated.
At baseline, the control group had lower levels of body mass (P = 0.000), BMI (P = 0.000), waist-hip ratio (P = 0.000), systolic blood pressure (P = 0.001), diagnostic blood pressure (P = 0.016) and triglycerides (P = 0.006), and a higher level of HDL cholesterol (P = 0.005) than the overweight group. When compared with those before the ILF-HC diet, total cholesterol (P < 0.05) and LDL cholesterol (P < 0.05) decreased, and insulin (P < 0.05) and HOMA-IR (P < 0.05) increased in both the control group and the overweight group after the LF-HC diet. Increased triglycerides (P = 0.000) were observed only in the control subjects, and HDL cholesterol (P = 0.018) increased only in the overweight subjects after the LF-HC diet.
The responses of serum TG and HDL-C to the LF-HC diet are related to BMI in healthy youth.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 01/2012; 43(1):9-14.
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ABSTRACT: To investigate the effects of the Gln27Glu polymorphism of beta2-adrenergic receptor (beta2AR) on serum lipid and apolipoprotein ratios and its interaction with high-carbohydrate/low-fat (HC/LF) diet on the ratios in healthy youth.
After on a washout diet for seven days, fifty six healthy young subjects were given the HC/LF diet for six days. The 12 hour-fasting serum lipids and apolipoproteins (apo) AI and B100 were measured on the 1st, the 8th and the 14th days. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, LDL-C/HDL-C and apoAI/apoB100 were calculated. The polymorphism of Gln27Glu was analyzed by PCR-RFLP method.
No significant differences were found of the lipid and apolipoprotein ratios at baseline and before the HC/LF diet between the subjects with the CC genotype (wide type) and the G carriers (mutation carriers) in the whole study sample or the males and the females separately. The G carriers had a significantly higher level of log (TG/HDL-C) (P=0.038) than the subjects with the CC genotype did after the HC/LF diet in the whole study sample but not in the males and the females separately. Significant decreases of LDL-C/HDL-C and TC/HDL-C were observed in all the subjects after the HC/LF diet when compared with those before the HC/LF diet (P<0.05), but a significant increase of apoAI/apoB100 (P=0.021) only in the subjects with the CC genotype. When gender was taken into account, significant decreases of LDL-C/HDL-C and TC/HDL-C were found after the HC/ LF diet in the male subjects regardless of genotypes (P<0.05). Significant increases of TG/HDL-C and log (TG/ HDL-C) and a significant decrease of TC/HDL-C were found in all the female subjects (P<0.05), while a significant decrease of LDL-C/HDL-C (P=0.037) was only observed in the female subjects with the CC genotype.
The G allele of beta2AR Gln27Glu variation can inhibit the decrease of LDL-C/HDL-C in females after HC/LF diet intervention.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 11/2011; 42(6):775-9.
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ABSTRACT: Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) diet may affect the ratios of serum lipids and apolipoproteins (apo) differently in subjects with different genotypes of the SstI polymorphism in the apoCIII gene (APOC3). Fifty-six healthy university students (27 males and 29 females, 22.89 ± 1.80 years) were given a washout diet of 54% carbohydrate for 7 days, followed by a high-CHO diet of 70% carbohydrate for 6 days without total energy restriction. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apoB100, apoAI, and the APOC3 SstI polymorphism were analyzed. The ratios of serum lipids and apoB100/apoAI were calculated. At baseline, the TG/HDL-C ratio was significantly higher in females, but not in males, with the S2 allele. The differences in the TG/HDL-C ratio between genotypes remained the same after the washout and the high-CHO diet in females. When compared with those before the high-CHO diet, the TC/HDL-C (male S2 carriers: 3.13 ± 1.00 vs 2.36 ± 0.65, P = 0.000; male subjects with the S1S1 genotype: 2.97 ± 0.74 vs 2.09 ± 0.55, P = 0.000; female S2 carriers: 2.68 ± 0.36 vs 2.24 ± 0.37, P = 0.004; female subjects with the S1S1 genotype: 2.69 ± 0.41 vs 2.09 ± 0.31, P = 0.000) and LDL-C/HDL-C (male S2 carriers: 1.44 ± 0.71 vs 1.06 ± 0.26, P = 0.012; male subjects with the S1S1 genotype: 1.35 ± 0.61 vs 1.01 ± 0.29, P = 0.005; female S2 carriers: 1.18 ± 0.33 vs 1.00 ± 0.18, P = 0.049; female subjects with the S1S1 genotype: 1.18 ± 0.35 vs 1.04 ± 0.19, P = 0.026) ratios were significantly decreased after the high-CHO diet regardless of gender and of genotype of the APOC3 SstI polymorphism. However, in female S2 carriers, the TG/HDL-C (1.38 ± 0.46 vs 1.63 ± 0.70, P = 0.039) ratio was significantly increased after the high-CHO diet. In conclusion, the high-CHO diet has favorable effects on the TC/HDL-C and LDL-C/HDL-C ratios regardless of gender and of genotype of the APOC3 SstI polymorphism. Somehow, it enhanced the adverse effect of the S2 allele on the TG/HDL-C ratio only in females.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas / Sociedade Brasileira de Biofisica ... [et al.] 06/2011; 44(6):524-30. · 1.08 Impact Factor
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ABSTRACT: To investigate the interaction of the C-60G polymorphism of hormone sensitive lipase gene (LIPE) with a high carbohydrate (high-CHO) diet on plasma lipids and glucose in a young and healthy Chinese Han population.
27 males and 29 females were given a washout diets of 31% fat, 54% carbohydrate and 15% protein for 7 days, followed by the high-CHO diet of 15% fat, 70% carbohydrate and 15% protein for 6 days, without total energy restriction. Plasma lipid profiles, glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-IR) and body mass index (BMI) at baseline, before and after the high-CHO diets as well as the LIPE C-60G polymorphism were analyzed.
The females with the CC genotype had significantly higher levels of high-density lipoprotein cholesterol (HDL-C) (P < 0.01, P < 0.05) and apolipoprotein A-I (apo A-I ) (P < 0.01, P < 0.01) than the males with the same genotype both before and after the high-CHO diet. After the high-CHO diet, only the females with the CC genotype had significantly higher levels of total cholesterol (TC) (P < 0.01) and low-density lipoprotein cholesterol (LDL-C) (P < 0.05) than their male counterparts. Compared with those before the high-CHO diet, the males with the CC genotype had significantly lower levels of BMI (P < 0.05), TC (P < 0.01) and LDL-C (P < 0.01), but significantly higher levels of HDL-C (P < 0.01) and apo A-I (P < 0.05) after the high-CHO diet. The males with the CG genotype experienced significant decreases of BMI (P < 0.05) and TC (P < 0.01). The females with the CC genotype experienced significant decreases of TC (P < 0.01) and LDL-C (P < 0.01) but significant increases of triglyceride (TG) (P < 0.01) and insulin (P < 0.05). The females with the CG genotype experienced a significant decrease of TC (P < 0.05) but a significant increase of TG (P < 0.05).
LIPE C-60G variation can inhibit the decrease of LDL-C and the increases of HDL-C and apo A-I in young healthy males induced by the high-CHO diet, and can inhibit the decrease of LDL-C and the increase of insulin in young healthy females induced by the same diet.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 05/2011; 42(3):335-9.
