Publications (2)0 Total impact
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Article: [Analysis of DNA methylation in different chicken tissues with MSAP].
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ABSTRACT: With methylation sensitive amplified polymorphism (MSAP), the DNA methylation levels and patterns of CCGG sites in genomes was analyzed among four different tissues and between parents and offsprings from three groups of adult chicken, White Leghorn, White Plymouth Rock, and their F1 hybrids. The results indicated that the degree of methylation was approximate 29.7% in muscle, 27.5% in liver, 27.5% in heart, and 26.1% in kidney. There was significantly different in the level of methylation in the 3 different groups and in 4 different tissues (P<0.05). The fully-methylated sites were less than the hemi-methylated sites among the 4 tissues, which was different from that of plants. The two tissue-specific MSAP fragments were isolated, sequenced, and characterized, both of which were located in the coding regions. These results clearly demonstrated that there was difference in the methylation level among various tissues and different groups, which suggested that the genetic factor may have effect on the individual methylation level.Hereditas (Beijing) 06/2011; 33(6):620-6. -
Article: [Comparative assessment of SSR diversity in aromatic rice germplasm].
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ABSTRACT: The genetic diversity in 370 aromatic rice germplasms introduced from home and abroad, was investigated by using a total of 60 SSR markers. 361 alleles were detected, with the number of alleles (Na) per loci ranging from 2 to 10. The Nei's genetic diversity index (He) among loci varied widely from 0.104 at RM308 to 0.885 at RM2634 with an average value of 0.663. There was an obvious difference in SSR allelic diversity between indica and japonica rice. Indica rice showed more variation than japonica rice both for Na and He. Meanwhile, the genetic diversity of the landraces was higher than that of the improved varieties, and Na of the improved varieties was 86.5% of the landraces. Analysis of molecular variance indicated that 43.08% of the variation was from genetic differences between subspecies. It was also demonstrated that the genetic differentiation among different rice ecological regions ranged from 1.69% to 14.40%. There was significant differentiation between Southern China and Southwestern China, Central China and Southwestern China, respectively. Cluster analysis showed that 370 aromatic rice varieties were mainly classified into indica and japonica, and aromatic rice varieties from the same areas or the neighboring provinces were basically assigned to the same group.Hereditas (Beijing) 05/2009; 31(4):412-9.
