Jorge Arturo Fernández-Silva

Justus-Liebig-Universität Gießen, Gießen, Hesse, Germany

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Publications (2)2.18 Total impact

  • Jorge Arturo Fernández-Silva, Amir Abdulmawjood, Ömer Akineden, Michael Bülte
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    ABSTRACT: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of Johne's disease or paratuberculosis of ruminants and has been associated with Crohn's disease in humans. In this study, the genotypes of MAP obtained so far in South American countries using a combination of the subtyping methods Mycobacterial Interspersed Repeats Units-Variable Number of Tandem Repeats (MIRU-VNTR) and Multilocus Short Sequence Repeats (MLSSR) were analyzed. Through this analysis, seven different MIRU-VNTR genotypes and seven MLSSR genotypes have been detected. If both methods were combined, nine different genotypes were found. Results revealed the predominance of MIRU-VNTR genotype 1 (INMV 1) and MLSSR genotype A (7 g-10 g-4ggt) among MAP isolates from different host species in South America. These predominant MAP genotypes are also commonly detected in Europe and the United States. This predominance could be the result of higher animal infection ability or better culturability on solid media used for isolation. Further studies on molecular epidemiology of MAP must be carried out in South America to increase our knowledge of the global distribution of MAP.
    Tropical Animal Health and Production 02/2012; 44(6):1123-6. · 1.09 Impact Factor
  • Jorge Arturo Fernández-Silva, Amir Abdulmawjood, Omer Akineden, Michael Bülte
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    ABSTRACT: The objective of this study is the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by serum enzyme-linked immunosorbent assay (ELISA), fecal polymerase chain reaction (PCR), and fecal culture in Colombian dairy herds. Serum and fecal samples from asymptomatic cows (n = 307) of 14 dairy herds were tested for MAP by an unabsorbed ELISA test (ELISA-A). Serum and fecal samples from positive ELISA-A animals (n = 31) were further tested by an absorbed ELISA test (ELISA-B) and PCR. Fecal samples from animals of herds positive by ELISA-A and PCR (n = 105) were inoculated onto three different culture media. ELISA-A produced positive results in 10% of the serum samples and 71% of the herds. ELISA-B and PCR results were positive in two and six serum and fecal samples from positive ELISA-A animals, respectively. Fecal samples were negative for MAP on all culture media. The results of this study confirmed the presence of MAP in local dairy herds and the difficulties of MAP detection in asymptomatic animals by ELISA, PCR, and fecal culture.
    Tropical Animal Health and Production 04/2011; 43(8):1501-7. · 1.09 Impact Factor

Publication Stats

4 Citations
2.18 Total Impact Points

Institutions

  • 2012
    • Justus-Liebig-Universität Gießen
      • Institut für Tierärztliche Nahrungsmittelkunde
      Gießen, Hesse, Germany
  • 2011
    • University of Antioquia
      • Facultad de Ciencias Agrarias
      Antioquia, Departamento de Antioquia, Colombia