Heather L A Robson

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Publications (2)5.33 Total impact

  • Article: Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae).
    Michelle Waycott, Brendan L Jones, Jent Kornelis Van Dijk, Heather L A Robson, Ainsley Calladine
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    ABSTRACT: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
    American Journal of Botany 04/2011; 98(4):e81-3. · 2.66 Impact Factor
  • Article: Isolation and characterization of microsatellite loci in Santalum lanceolatum and Santalum leptocladum (Santalaceae).
    Brendan L Jones, Michelle Waycott, Heather L A Robson, Ainsley Calladine, Tony Page
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    ABSTRACT: • Premise of the study: Microsatellite primers were developed for the first time in the native Australian sandalwood species Santalum lanceolatum. • Methods and Results: Using an enrichment cloning protocol, five novel polymorphic codominant loci were developed and characterized in S. lanceolatum and S. leptocladum. In addition to these, three existing microsatellite loci from other sandalwood species were successfully amplified and characterized for S. lanceolatum and S. leptocladum. Among the eight loci, allelic diversity ranged from 4 to 29. • Conclusions: Primers will be useful for studies of clonality, genetic diversity and spatial genetic structure in wild populations. When coupled with other molecular techniques will help investigate the relationship between S. lanceolatum and S. leptocladum, species of commercial and conservation interest.
    American Journal of Botany 10/2010; 97(10):e97-8. · 2.66 Impact Factor
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