Publications (2)5.04 Total impact
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Article: Trehalose overproduction affects the stress tolerance of Kluyveromyces marxianus ambiguously.
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ABSTRACT: A thermotolerant Kluyveromyces marxianus mutant was developed by exposing yeast cultures repeatedly to 48°C incubation temperature, and the strain was characterized with a significantly increased trehalose content. Unexpectedly, the strain was sensitive to alcohol, osmotic and oxidative stress, which correlated with the increases in the trehalose concentrations. Intracellular glutathione levels declined in both wild-type and mutant cells when exposed to elevating incubation temperatures. Finally, we reached the surprising conclusion that neither trehalose nor glutathione metabolisms should be aimed at in future strain development programs with K. marxianus.Bioresource technology 07/2011; 102(14):7232-5. · 4.25 Impact Factor -
Article: Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans.
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ABSTRACT: Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli. Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns.Acta Microbiologica et Immunologica Hungarica 10/2008; 55(3):351-61. · 0.79 Impact Factor
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Institutions
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2008–2011
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University of Debrecen
- Department of Microbial Biotechnology and Cell Biology
Debrecen, Hajdu-Bihar, Hungary
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