Emily Rogena

Publications (4)19.78 Total impact

• Article: Targeted genomic sequencing of pediatric Burkitt lymphoma identifies recurrent alterations in anti-apoptotic and chromatin-remodeling genes.

  Lisa Giulino-Roth, Kai Wang, Theresa Y Macdonald, Susan Mathew, Yifang Tam, Maureen T Cronin, Gary Palmer, Norma Lucena-Silva, Francisco Pedrosa, Marcia Pedrosa, [......], Lorenzo Leoncini, Cristiana Bellan, Emily Rogena, Kerice A Pinkney, Mark A Rubin, Raul C Ribeiro, Roman Yelensky, Wayne Tam, Philip J Stephens, Ethel Cesarman
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  ABSTRACT: To ascertain the genetic basis of pediatric Burkitt lymphoma (pBL) we performed clinical-grade next generation sequencing (NGS) of 189 cancer-related genes on 29 formalin-fixed paraffin embedded (FFPE) primary pBL samples. Ninety percent of cases had at least one mutation or genetic alteration, most commonly involving MYC and TP53. EBV(-) cases were more likely than EBV(+) cases to have multiple mutations (p<0.0001). Alterations in tumor-related genes not previously described in BL were identified. Truncating mutations in ARID1A, a member of the SWI/SNF nucleosome remodeling complex, were seen in 17% of cases. MCL1 pathway alterations were found in 22% of cases and confirmed in an expanded panel. Other clinically relevant genomic alterations were found in 20% of cases. Our data suggest the role of MCL1 and ARID1A in BL pathogenesis and demonstrate that comprehensive genomic profiling may identify additional treatment options in refractory disease.
  Blood 10/2012; · 9.90 Impact Factor
• Source

  Available from: Ian Magrath

  Article: Diagnosis of Burkitt lymphoma using an algorithmic approach - applicable in both resource-poor and resource-rich countries.

  Kikkeri N Naresh, Hazem A H Ibrahim, Stefano Lazzi, Patricia Rince, Monica Onorati, Maria R Ambrosio, Chrystèle Bilhou-Nabera, Furrat Amen, Alistair Reid, Michael Mawanda, Valeria Calbi, Martin Ogwang, Emily Rogena, Bessie Byakika, Shahin Sayed, Emma Moshi, Amos Mwakigonja, Martine Raphael, Ian Magrath, Lorenzo Leoncini
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  ABSTRACT: Distinguishing Burkitt lymphoma (BL) from B cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma (DLBCL) and BL (DLBCL/BL), and DLBCL is challenging. We propose an immunohistochemistry and fluorescent in situ hybridization (FISH) based scoring system that is employed in three phases - Phase 1 (morphology with CD10 and BCL2 immunostains), Phase 2 (CD38, CD44 and Ki-67 immunostains) and Phase 3 (FISH on paraffin sections for MYC, BCL2, BCL6 and immunoglobulin family genes). The system was evaluated on 252 aggressive B-cell lymphomas from Europe and from sub-Saharan Africa. Using the algorithm, we determined a specific diagnosis of BL or not-BL in 82%, 92% and 95% cases at Phases 1, 2 and 3, respectively. In 3·4% cases, the algorithm was not completely applicable due to technical reasons. Overall, this approach led to a specific diagnosis of BL in 122 cases and to a specific diagnosis of either DLBCL or DLBCL/BL in 94% of cases that were not diagnosed as BL. We also evaluated the scoring system on 27 cases of BL confirmed on gene expression/microRNA expression profiling. Phase 1 of our scoring system led to a diagnosis of BL in 100% of these cases.
  British Journal of Haematology 07/2011; · 4.94 Impact Factor
• Article: Lymphomas in sub‐Saharan Africa – what can we learn and how can we help in improving diagnosis, managing patients and fostering translational research?

  Kikkeri N. Naresh, Martine Raphael, Leona Ayers, Nina Hurwitz, Valeria Calbi, Emily Rogena, Shahin Sayed, Omar Sherman, Hazem A.H. Ibrahim, Stefano Lazzi, [......], Bessie Byakika, Emma Moshi, Muheez Durosinmi, Babatunde J. Olasode, Olayiwola A. Oluwasola, Effiong E. Akang, Yetunde Akenòva, Melissa Adde, Ian Magrath, Lorenzo Leoncini
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  ABSTRACT: Approximately 30 000 cases of non-Hodgkin lymphoma (NHL) occur in the equatorial belt of Africa each year. Apart from the fact that Burkitt lymphoma (BL) is very common among children and adolescents in Africa and that an epidemic of human immunodeficiency virus (HIV) infection is currently ongoing in this part of the world, very little is known about lymphomas in Africa. This review provides information regarding the current infrastructure for diagnostics in sub-Saharan Africa. The results on the diagnostic accuracy and on the distribution of different lymphoma subsets in sub-Saharan Africa were based on a review undertaken by a team of lymphoma experts on 159 fine needle aspirate samples and 467 histological samples during their visit to selected sub-Saharan African centres is presented. Among children (<18 years of age), BL accounted for 82% of all NHL, and among adults, diffuse large B-cell lymphoma accounted for 55% of all NHLs. Among adults, various lymphomas other than BL, including T-cell lymphomas, were encountered. The review also discusses the current strategies of the International Network of Cancer Treatment and Research on improving the diagnostic standards and management of lymphoma patients and in acquiring reliable clinical and pathology data in sub-Saharan Africa for fostering high-quality translational research.
  British Journal of Haematology 06/2011; 154(6):696 - 703. · 4.94 Impact Factor
• Article: Reactivated Toxoplasmosis Presenting with Non-tender Hepatomegaly in a Patient with HIV Infection

  Hudson Lodenyo, Susan M. Sitati, Emily Rogena
  African Journal of Health Sciences (ISSN: 1022-9272) Vol 14 Num 1-2.