C L Jiang

Jilin University, Yung-chi, Jilin Sheng, China

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Publications (2)2.16 Total impact

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    ABSTRACT: Human influenza viruses are major concern as the leading cause of global pandemics. In infecting cells, they preferentially bind to sialyloligosaccharides containing terminal N-acetyl sialic acid linked to galactose by an α-2,6-linkage (NeuAcα2,6Gal). The amount of NeuAcα2,6Gal in Vero cells, which are predominantly used for production of influenza vaccines over the past 30 years, may not be as high as that in epithelial cells of human respiratory tract, what leads to the suboptimal virus growth in Vero cells. In this study, we stably transfected Vero cells with cDNA of human α-2,6-sialyltransferase (SIAT1), an enzyme catalyzing α-2,6-sialylation of galactose on glycoproteins. Overexpression of SIAT1 in the transfected Vero cells (Vero-SIAT1 cells) was confirmed by Western blot analysis and immunofluorescence microscopy. Vero-SIAT1 cells expressed 7 times higher amounts of NeuAcα2,6Gal, but 3 times lower amounts of NeuAcα2,3Gal as compared to parental Vero cells. Furthermore, the influenza viruses A (H1N1 and H3N2) and B grew in Vero-SIAT1 cells to the higher titers than in Vero cells. Taken together, these results imply that Vero-SIAT1 cells are useful not only for the propagation of human influenza viruses, but also for the preparation of influenza vaccines.
    Acta virologica 01/2011; 55(2):147-53. DOI:10.4149/av_2011_02_147 · 1.04 Impact Factor
  • H LI, CL Jiang, XH Yu, YG Wu, W LI, W KONG
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    ABSTRACT: The effect of repressors on ion channel gene expression was studied. The hKv4. 3 promoter and the sequence (+2— + 160, S160) of5′-UTR of the hKv4. 3 gene was cloned into the pB-gal-Basic vector. The transient expression of the pB-gal vector and the analysis of the relative activity of B-galactosidase were carried out. The analysis of the mRNA level was carried out with the RT-PCR method. S160 could intensively repress the expression of the hKv4. 3 gene with position-specificity. The level of mRNA did not alter obviously. A repressor (S160), in 5′-UTR of the hKv4. 3 gene was found and its repression to gene expression may play a role in the post-transcription process.
    Chemical Research in Chinese Universities 03/2006; 22(2):197-200. DOI:10.1016/S1005-9040(06)60075-5 · 1.12 Impact Factor