Bernadeta Dobosz

Publications (2)1.51 Total impact

• Source

  Available from: Ryszard Krzyminiewski

  Article: Free Radicals, Salicylic Acid and Mycotoxins in Asparagus After Inoculation with Fusarium proliferatum and F. oxysporum.

  Bernadeta Dobosz, Kinga Drzewiecka, Agnieszka Waskiewicz, Lidia Irzykowska, Jan Bocianowski, Zbigniew Karolewski, Marian Kostecki, Zdzislaw Kruczynski, Ryszard Krzyminiewski, Zbigniew Weber, Piotr Golinski
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  ABSTRACT: Electron paramagnetic resonance was used to monitor free radicals and paramagnetic species like Fe, Mn, Cu generation, stability and status in Asparagus officinalis infected by common pathogens Fusarium proliferatum and F. oxysporum. Occurrence of F. proliferatum and F. oxysporum, level of free radicals and other paramagnetic species, as well as salicylic acid and mycotoxins content in roots and stems of seedlings were estimated on the second and fourth week after inoculation. In the first term free and total salicylic acid contents were related to free radicals level in stem (P = 0.010 and P = 0.033, respectively). Concentration of Fe(3+) ions in porphyrin complexes (g = 2.3, g = 2.9) was related to the species of pathogen. There was no significant difference between Mn(2+) concentrations in stem samples; however, the level of free radicals in samples inoculated with F. proliferatum was significantly higher when compared to F. oxysporum.
  Applied Magnetic Resonance 09/2011; 41(1):19-30. · 0.75 Impact Factor
• Source

  Available from: Ryszard Krzyminiewski

  Article: EPR Study of Iron Ion Complexes in Human Blood.

  Ryszard Krzyminiewski, Zdzisław Kruczyński, Bernadeta Dobosz, Anna Zając, Andrzej Mackiewicz, Ewa Leporowska, Sandra Folwaczna
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  ABSTRACT: Electronic states of iron ion complexes in human blood from patients with melanoma have been investigated by electron paramagnetic resonance (EPR). The measurements were performed at liquid nitrogen temperature (77 K) on an X-band EPR spectrometer. Numerous types of iron paramagnetic centers have been identified. In several kinds of protein complexes exemplified by methemoglobin, transferrin or ferritin, various forms of trivalent iron have been found. Three groups of patients with typical EPR spectra have been individualized. These groups differed in types and concentration of paramagnetic centers in peripheral blood. A good correlation has been found between the EPR results, the total iron ion complexes concentration and transferrin saturation.
  Applied Magnetic Resonance 05/2011; 40(3):321-330. · 0.75 Impact Factor