Françoise Borcard, Aurélien Godinat,
Davide Staedler,
Horacio Comas Blanco,
Anne-Laure Dumont,
Catherine Chapuis-Bernasconi,
Corinne Scaletta,
Lee Ann Applegate,
Franziska Krauss Juillerat,
Urs T Gonzenbach,
Sandrine Gerber-Lemaire,
Lucienne Juillerat-Jeanneret
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ABSTRACT: The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne derivatives with cellular azido-expressing proteins. The incorporation via a tetraethylene glycol linker of a dipeptide and a reporter biotin allowed the proof of concept for the introduction of cell-specific peptide ligands and allowed us to follow the reaction in living cells. Tuning the conditions of the click reaction resulted in chemical functionalization of living human fetal osteoblasts with excellent cell survival.
Bioconjugate Chemistry 06/2011; 22(7):1422-32. · 4.93 Impact Factor
