Ying Xu

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Publications (2)5.39 Total impact

  • Article: Osterix is Required for Sonic Hedgehog-Induced Osteoblastic MC3T3-E1 Cell Differentiation.
    Ye Tian, Ying Xu, Qin Fu, Yufeng Dong
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    ABSTRACT: It has been shown that hedgehog (Hh) signaling plays an important role during bone development. However, the mechanism(s) by which Hh stimulates osteoblast differentiation are not fully elucidated. This study was performed to examine if Sonic hedgehog (Shh) affects osteoblast differentiation in osteoblastic MC3T3-E1 cells and determine the exact role of osterix (Osx) involved in Hh-induced osteoblast differentiation. Our real-time RT-PCR result shows that Shh significantly induced osteoblast differentiation by up-regulation of osteocalcin, alkaline phosphatase, bone sialoprotein, Type I collagen, runt-related transcription factor 2 (Runx2), and Osx RNA expression in MC3T3-E1 cells. ALP protein activity, Osx protein expression, as well as Osx promoter activity was also enhanced by Shh treatment in cell culture. Interestingly, Shh-induced Osx up-regulation was only slightly affected by knocking down Runx2 using siRNA in cells within 3 days of culture, however, knocking down Osx expression in cells totally blocked Shh-induced osteoblast differentiation. These findings demonstrate for the first time that Shh stimulates early osteoblast differentiation mainly through up-regulation of the expression of Osx in osteoblastic MC3T3-E1 cells in both Runx2-dependent and Runx2-independent manner.
    Cell biochemistry and biophysics 05/2012; · 3.34 Impact Factor
  • Article: Parathyroid hormone regulates osteoblast differentiation in a Wnt/β-catenin-dependent manner.
    Ye Tian, Ying Xu, Qin Fu, Ming He
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    ABSTRACT: Intermittent parathyroid hormone (PTH) administration shows an anabolic effect on bone. However, the mechanisms are not fully studied. Recent studies suggest that Wnt signaling is involved in PTH-induced bone formation. The current study was to examine if Wnt/β-catenin pathway is required during PTH-induced osteoblast differentiation. Osteoblastic MC3T3-E1 cells were treated with human PTH (1-34) (hPTH [1-34]) and expression levels of osteoblast differentiation markers were detected by real-time PCR. RNA levels of β-catenin, Runx2, Osteocalcin, Alkaline phosphatase, and Bone sialoprotein were significantly up-regulated after treatment with 10(-8) M of hPTH (1-34) for 6 h. Alkaline phosphatase activity and protein expression of β-catenin were also increased after 6 days of intermittent treatment with hPTH (1-34) in MC3T3-E1 cells. hPTH (1-34) significantly enhanced Topflash Luciferase activity after 6 h of treatment. More important, PTH-induced Alkaline phosphatase activity was significantly inhibited by knocking down β-catenin expression in cells using siRNA. Real-time RT-PCR results further showed down regulation of Runx2, Osteocalcin, Alkaline phosphatase, Bone sialoprotein gene expression in β-catenin siRNA transfected cells with/without PTH treatment. These results clearly indicate that PTH stimulates Wnt/β-catenin pathway in MC3T3-E1 cells and osteoblast differentiation markers expression was up-regulated by activation of Wnt/β-catenin signaling. Our study demonstrated that PTH-induced osteoblast differentiation mainly through activation of Wnt/β-catenin pathway in osteoblastic MC3T3-E1 cells.
    Molecular and Cellular Biochemistry 05/2011; 355(1-2):211-6. · 2.06 Impact Factor
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