Publications (2)0 Total impact
Article: [Improvement in peripheral blood T cell subset imbalance in a house with an elimination system for various allergens].[show abstract] [hide abstract]
ABSTRACT: We analyzed the change of peripheral blood T cell subsets after moving into a house with a Healthy Air system (HAS) elimination system for various allergens, e.g. pollens, house dusts, etc. The 20 subjects were divided into an allergic group (13 subjects) and control group (7 subjects). We measured complete blood counts (CBC), white blood cell differentiation (DIFF), CD4/CD8 ratio, Th1/Th2 ratio, and percentage of CD4+CD25+T-cells and regulatory T-cells in peripheral blood, and these data were compared before and 3 and 6 months after moving into the HAS house. There was no significant difference in CBC, DIFF, CD4/CD8 ratio, and Th1/Th2 ratio before and after the move. The mean levels and 95% confidence interval of CD4+CD25+T-cells in the allergic group were as follows: before, 16.66% (12.99-20.34%); at 3 months, 13.86% (10.49-17.22%); and at 6 months, 12.66% (9.28-16.05%), respectively. Those in the control group were as follows: before, 13.60% (5.27-21.93%); at 3 months, 12.51%(5.41-19.61%); and at 6 months, 11.77% (3.93-19.61%), respectively. CD4+CD25+T-cells were significantly decreased at 6 months after the move compared to before the move in the allergic group (p < 0.01). However, there was no significant difference between before and after the move in the control group. The mean levels of regulatory T-cells were not different between before and after the move in both groups. The mean level of CD4+CD25+T-cells in subjects that had improved allergic condition was significantly decreased at 6 months after the move compared to before the move (p < 0.05). These results suggest that decreases in allergens in the home environment may affect lymphocyte subsets.Rinsho byori. The Japanese journal of clinical pathology 05/2012; 60(5):414-21.
Article: [Evaluation of mental stress tests among medical students based on salivary sample collected just before the national license examination].[show abstract] [hide abstract]
ABSTRACT: We investigated salivary amylase (sAMY) and chromogranin A (sCgA) in students before the national license examination in order to investigate the relationship between stress biomarkers and the Profile of Mood States (POMS) psychological test. Fifty-one medical students that provided informed consent were tested for sAMY activity and sCgA concentration by means of the amylase monitor method (NIPRO) and an ELISA kit (Yanaihara), respectively. The POMS psychology test (shortened form) was purchased from Chiba Test Center, and all students fully answered the lifestyle questionnaires. Based on answers to the questionnaires, students were divided by mental burden into three groups: I all"; II "large"; and III "very large". Scores for "T-A", "D" and "A-H" on the POMS test were significantly higher in groups II and III when compared with group I. Mean TMD scores calculated from the 6 items on the POMS test increased significantly with mental burden. The mean levels and 95% confidence interval (CI) of sAMY activity in the 3 groups were as follows: I, 27.7 (95% CI: 13.7-41.7) KIU/L; II, 29.1(95% CI: 22.4-35.7) KIU/L; and III, 26.9 (95% CI: 15.2-38.6) KIU/L. Mean sCgA concentrations were: I, 4.4(95% CI: 0-9.4) pmol/mg; II, 4.3(95% CI: 2.0-6.7) pmol/mg; and III, 10.9 (95% CI: 6.8-15.0) pmol/mg. There were no significant differences between these mean levels. However, Spearman's rank-correlation coefficient analysis for "T-A", sAMY and sCgA showed a stronger correlation between "T-A" and sCgA than between "T-A" and sAMY (p < 0.05). In conclusion, sCgA was more useful biomarker to evaluate the psychological stress before the national license examination than sAMY.Rinsho byori. The Japanese journal of clinical pathology 02/2011; 59(2):138-43.