Xingfei Pan

Publications (3)10.13 Total impact

• Article: Increased interleukin-32 expression in chronic hepatitis B virus-infected liver.

  Qihuan Xu, Xingfei Pan, Xin Shu, Hong Cao, Xuejun Li, Ka Zhang, Jianxi Lu, Yong Zou, Xueling Li, Hongcan Liu, Yeqiong Zhang, Daofeng Yang, Qin Ning, Guanxin Shen, Gang Li
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  ABSTRACT: The role of interleukin-32 (IL-32) in chronic hepatitis B (CHB) remains unclear. In order to identify the role of IL-32 in CHB, we detected the expression levels of IL-32 in liver samples of CHB patients and analyzed the correlation between IL-32 and liver inflammation/fibrosis. Real-time PCR and immunohistochemistry were used to detect the expression levels of IL-32 in liver tissues of patients with CHB. The correlation between hepatic IL-32 expression and the severity of liver inflammation/fibrosis was assessed using Spearman's correlation. Hepatic IL-32 expression was increased in CHB patients and increased with the severity of liver inflammation/fibrosis. Moreover, hepatic IL-32 expression was significantly positively correlated with serum ALT level and negatively related with serum ALB level. Our results suggest that IL-32 could be implicated in HBV-related liver inflammation/fibrosis. We believe that IL-32 might play important roles in the pathogenesis of CHB.
  The Journal of infection 06/2012; 65(4):336-42. · 4.13 Impact Factor
• Article: Novel AAV-based genetic vaccines encoding truncated dengue virus envelope proteins elicit humoral immune responses in mice.

  Xueling Li, Hong Cao, Qiang Wang, Biao Di, Ming Wang, Jianxi Lu, Lijie Pan, Li Yang, Mingzhu Mei, Xingfei Pan, Gang Li, Lili Wang
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  ABSTRACT: The envelope protein of dengue virus is involved in host cell attachment for entry and induction of protective immunity. Current efforts are focused on producing a tetravalent vaccine by mixing four monovalent vaccine components. In this work, we developed a genetic vaccine based on a novel adeno-associated viral (AAV) vector expressing the carboxy-terminal truncated envelope protein (79E) of dengue virus. The expression of the recombinant 79E protein in HEK 293 cells was confirmed by Western blot. Vectors packaged with novel AAV capsids (AAV2/8 or AAV2/rh32.33) were injected into C57BL/6 mice intramuscularly. Dengue virus antigen was produced in the mice and induced long-lasting antibody responses against the dengue virus still detectable 20 weeks after immunization. AAV2/8 vaccine induced higher anti-dengue virus antibody levels than AAV2/rh32.33 vaccine or AAV plasmid. Furthermore, the anti-dengue antibodies could neutralize homogeneous dengue virus. These results demonstrated that the AAV vaccines possessed appropriate immunogenicity and could be used for the development of an effective dengue vaccine.
  Microbes and Infection 05/2012; 14(11):1000-7. · 3.10 Impact Factor
• Article: Interleukin-32 expression induced by hepatitis B virus protein X is mediated through activation of NF-κB.

  Xingfei Pan, Hong Cao, Jianxi Lu, Xin Shu, Xiaojia Xiong, Xiaolv Hong, Qihuan Xu, Huifen Zhu, Gang Li, Guanxin Shen
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  ABSTRACT: HBV replicates noncytopathically in hepatocytes, but HBV or proteins encoded by HBV genome could induce cytokines, chemokines expression by hepatocytes. Moreover, liver damage in patients with HBV infection is immune-mediated and cytokines play important roles in immune-mediated liver damage after HBV infection. Interleukin-32 (IL-32) is a proinflammatory cytokine and plays a critical role in inflammation. However, the role of HBV in IL-32 expression remains unclear. In the present study, we demonstrate that hepatitis B virus protein X (HBx) increases IL-32 expression through the promoter of IL-32 at positions from -746 to +25 and in a dose-dependent manner. Furthermore, we demonstrate that increase of NF-κB subunits p65 and p50 in Huh7 cells also augments IL-32 expression, and the NF-κB inhibitor blocks the effect of HBx on IL-32 induction. These results indicate that NF-κB activation is required for HBx-induced IL-32 expression. In conclusion, IL-32 is induced by HBx in Huh7 cells. Our results suggest that IL-32 might play an important role in inflammatory response after HBV infection.
  Molecular Immunology 07/2011; 48(12-13):1573-7. · 2.90 Impact Factor