Spartaco Astolfi-Filho

Publications (4)10.89 Total impact

• Article: Microsatellite loci for population and parentage analysis in the Amazon River dolphin (Inia geoffrensis de Blainville, 1817).

  Waleska Gravena, Tomas Hrbek, Vera M S DA Silva, Spartaco Astolfi-Filho, Izeni P Farias
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  ABSTRACT: We developed specific primers for microsatellite DNA regions for the Amazon River dolphin or boto Inia geoffrensis, for use in population and conservation genetic studies. We also tested their transferability for two other species, Pontoporia blainvillei (sister taxon of I. geoffrensis) and Sotalia guianensis. A total of 12 microsatellite loci were polymorphic for the boto. An additional 25 microsatellite loci previously isolated from other cetacean species were also tested in the boto. The 26 polymorphic microsatellite loci indicate they will be excellent markers for studies of population structure and kinship relations of the boto.
  Molecular Ecology Resources 03/2009; 9(2):600-603. · 3.06 Impact Factor
• Source

  Available from: evoamazon.net

  Article: Isolation and characterization of microsatellite markers for the ornamental discus fish Symphysodon discus and cross-species amplification in other Heroini cichlid species.

  Manuella Villar Amado, Tomas Hrbek, Waleska Gravena, Cleiton Fantin, Enedina Nogueira DE Assunção, Spartaco Astolfi-Filho, Izeni P Farias
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  ABSTRACT: The discus fishes (Symphysodon spp.) are economically important ornamental species. Thirteen microsatellite markers were developed from a CT(12) - and CA(12) -enriched whole genomic DNA library of Symphysodon discus. Allelic variability was tested on 44 individuals of two species (S. discus and S. aequifasciatus). Allelic richness ranged from two to 11 alleles per locus and observed heterozygosities from 0.083 to 0.998. All loci were at Hardy-Weinberg equilibrium, and no pair of loci showed linkage disequilibrium within a species. Cross-species amplification was also successfully performed in the Neotropical cichlids Uaru amphiacanthoides, Hoplarchus psittacus, Hypselecara coryphaenoides, Pterophyllum sp., Mesonauta sp. and Heros sp.
  Molecular Ecology Resources 11/2008; 8(6):1451-3. · 3.06 Impact Factor
• Source

  Available from: evoamazon.net

  Article: Microsatellite DNA markers for Podocnemis unifilis, the endangered yellow‐spotted Amazon River turtle

  CLEITON FANTIN, CECÍLIA F. CARVALHO, TOMAS HRBEK, JACK W. SITES JR, LUIZ A. S. MONJELÓ, SPARTACO ASTOLFI-FILHO, IZENI P. FARIAS
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  ABSTRACT: We developed specific primers for microsatellite DNA regions of Podocnemis unifilis and tested their utility in population genetic and paternity studies on the species and other closely related Amazonian chelonians. Seventeen microsatellite loci were polymorphic in P. unifilis and all, plus two monomorphic microsatellites in P. unifilis, were polymorphic in at least one additional chelonian species, including Peltocephalus dumeriliana.
  Molecular Ecology Notes 10/2007; 7(6):1235 - 1238. · 2.38 Impact Factor
• Source

  Available from: Iracilda Sampaio

  Article: Isolation and characterization of DNA microsatellite primers for Cynoscion acoupa, the most exploited sciaenid fish along the coast of Brazil

  IZENI P. FARIAS, LARISSA B. MUNIZ, SPARTACO ASTOLFI-FILHO, IRACILDA SAMPAIO
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  ABSTRACT: The northern mangrove coast of Brazil comprises the Brazilian states of Amapá, Pará and Maranhão. Fishes from the coastal mangrove region form a principal component of the local fishery, and account for 58% of all fish landed. The most commonly landed fishes are those of the family Sciaenidae. Cynoscion acoupa is one of the most exploited sciaenid species, with over 600 tons landed each year. Therefore, we developed 17 microsatellite markers to investigate genetic variability and population structuring of this species along the coast of Brazil.
  Molecular Ecology Notes 08/2006; 6(3):660 - 663. · 2.38 Impact Factor