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ABSTRACT: Ultrasonic extraction (USE) of polysaccharide from cordyceps cephalosporium mycelia was introduced and compared with hot water extraction. Crude polysaccharides were investigated for morphological and thermodynamic properties. Two major fractions (USEP40-1, USEP70-1) were purified and investigated for IR spectra, molecular weights, monosaccharide compositions and antioxidant activities. The results suggested that USE gained higher extraction efficiency due to its intense extraction process. USE caused a more compact arrangement in crude polysaccharides. IR spectra of USEP40-1 and USEP70-1 were different at around 1746 cm(-1). The molecular weights of USEP40-1 and USEP70-1 were 61.4 kDa and 25.1 kDa. USEP40-1 and USEP70-1 were composed of D-mannose, D-glucose, D-galactose, L-rhamnose, and L-arabinose with molar ratio of 11.52:5.54:8.75:2.45:2.59 and 11.50:6.74:5.75:4.46:2.39, respectively. Different ABTS and hydroxyl radical scavenging activities were observed in USEP40-1 and USEP70-1.
International journal of biological macromolecules 05/2012; 51(1-2):64-9. · 2.37 Impact Factor
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ABSTRACT: Polysaccharides from cultivated Saussurea involucrata (CSIP) were purified, two major fractions (CSIP1-2 and CSIP2-3) were investigated for their molecular weights, monosaccharide compositions and in vitro antioxidant activities. The results suggested that the molecular weights of CSIP1-2 and CSIP2-3 were approximately 163.5 kDa and 88.6 kDa, respectively. CSIP1-2 was composed of glucose, galactose, xylose, rhamnose, arabinose and galacturonic acid with a molar ratio of 1.651:0.39:0.062:8.331:1.759:40.426. CSIP2-3 was composed of glucose, galactose, xylose, rhamnose, arabinose and galacturonic acid with a molar ratio of 0.762:0.657:0.112:5.587:0.318:44.655. Different scavenging activities on superoxide radical, DPPH radical and hydroxyl radical were observed in CSIP1-2 and CSIP2-3 at tested concentrations.
International journal of biological macromolecules 11/2011; 50(3):849-53. · 2.37 Impact Factor
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ABSTRACT: Polysaccharides were extracted from Asparagus officinalis. A novel ultrasonic circulating extraction (UCE) technology was applied for the polysaccharide extraction. Three-factor-three-level Box-Behnken design was employed to optimize ultrasonic power, extraction time and the liquid-solid ratio to obtain a high polysaccharide yield. The optimal extraction conditions were as follows: ultrasonic power was 600 W, extraction time was 46 min, the liquid-solid ratio was 35 mL/g. Under these conditions, the experimental yield of polysaccharides was 3.134%, which was agreed closely to the predicted value. The average molecular weight of A. officinalis polysaccharide was about 6.18×10(4) Da. The polysaccharides were composed of glucose, fucose, arabinose, galactose and rhamnose in a ratio of 2.18:1.86:1.50:0.98:1.53. Compared with hot water extraction (HWE), UCE showed time-saving, higher yield and no influence on the structure of asparagus polysaccharides. The results indicated that ultrasonic circulating extraction technology could be an effective and advisable technique for the large scale production of plant polysaccharides.
International journal of biological macromolecules 08/2011; 49(2):181-7. · 2.37 Impact Factor
