Priscilla D′Mello

Publications (2)0 Total impact

• Article: Antioxidant and antiinflammatory activity of Vitex negundo

  R Kulkarni, A Virkar, Priscilla D′Mello
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  ABSTRACT: Reactive oxygen species are implicated in various inflammatory disorders. Vitex negundo is mentioned in Ayurveda as useful in treating arthritic disorders. The present work was undertaken to evaluate the antioxidant potential and anti-inflammatory activity of the plant. The total methanol extract of the plant was standardized in terms of total polyphenols. The standardized extract in a dose of 100 mg/kg caused a comparable reduction in edema with that of diclofenac sodium (25 mg/kg) when evaluated for antiinflammatory activity by carrageenan-induced rat paw edema method. The extract also exhibited a strong free radical scavenging activity by 1,1-diphenyl-2-picrylhydrazyl method and caused a significant reduction in the formation of thiobarbituric acid reacting substances when evaluated for its lipid peroxidation inhibitory activity. The results strongly suggest that radical quenching may be one of the mechanisms responsible for its antiinflammatory activity.
  Indian Journal of Pharmaceutical Sciences. 01/2008;
• Article: Standardization of selected Indian medicinal herbal raw materials containing polyphenols as major phytoconstituents

  Y Palav, Priscilla D′mello
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  ABSTRACT: The fruits of Terminalia chebula, Terminalia bellerica and Emblica officinalis are important herbal raw materials containing polyphenols. They form the major constituents of widely used Ayurvedic formulations like Triphala churna . The extracts of these materials were standardized with respect to their total polyphenol contents as determined by Prussian blue method using gum acacia and phosphoric acid as stabilizers. The antioxidant activities were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and inhibition of lipid peroxide formation induced by Fe2+-ascorbate system. They were found to strongly correlate with total polyphenol contents. The EC50 value (µg/ml) for free radical scavenging activity by DPPH method and IC50 value (µg/ml) for lipid peroxidation inhibitory activity along with the total polyphenol contents can be used as quality control parameters for standardization of herbal raw materials containing polyphenols as major phytoconstituents.
  Indian Journal of Pharmaceutical Sciences. 01/2006;