Mousheng Liu

Kunming University of Science and Technology, Yün-nan, Yunnan, China

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Publications (11)21.5 Total impact

  • Qinghai Xia, Yaling Yang, Mousheng Liu
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    ABSTRACT: The present work describes a simple and rapid extraction approach based on vortex-assisted acid-induced anionic surfactant sodium dodecyl sulfate-mediated extraction (vortex-assisted acid-induced cloud point extraction) prior to spectrofluorometry for the determination of four fluoroquinolones, including norfloxacin, ciprofloxacin, sarafloxacin, and gatifloxacin, in water samples (hospital wastewater, river water, lake water, and water from the inlet of a wastewater treatment plant). Sodium dodecyl sulfate and concentrated hydrochloric acid (12 mol/L) were used as extractants. The four fluoroquinolones were concentrated in the surfactant-rich phase after phase separation and were determined by a spectrofluorophotometer. Some parameters affecting the extraction efficiency, such as the type and amount of surfactant, the amount of acid, vortex treatment, and centrifugation, were optimized systematically. Under the optimized conditions, the proposed method showed good analytical features with linearity for all analytes in a wide range of 0.045–0.90 µg/mL and coefficients of determination no less than 0.9901. The limits of detection (S/N = 3) were 0.007, 0.010, 0.009, and 0.013 µg/mL for norfloxacin, ciprofloxacin, sarafloxacin, and gatifloxacin, respectively; good precisions expressed as the relative standard deviations were in the range of 0.9–3.7% (n = 6); and average recoveries at three spiking levels ranged from 83.0% to 96.7%. The results of the proposed method are comparable to those obtained from the high-performance liquid chromatography method (t-test, p = 0.05) and are reliable for determination of fluoroquinolones in environmental water samples.
    Spectroscopy Letters 01/2014; 47(3). · 0.67 Impact Factor
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    ABSTRACT: Glucocorticoids contamination has become a big environmental issue in China and other developing countries, due to increasing needs in medical prescription and farming. However, no highly sensitive and precise methods have been reported to quantify glucocorticoids so far. In the past several years, supramolecular solvent-based vortex-mixed microextraction (SS-BVMME) has been shown to be effective. However, the mechanism of SS-BVMME is still unknown. In this report, a novel method has been proposed for rapid quantification of trace amount of glucocorticoids, beclomethasone dipropionate (BD), hydrocortisone butyrate (HB) and nandrolone phenylpropionate (NPP) in water samples from the Green Lake. This method is simple, safe and cost effective. It contains two steps: supramolecular solvent-based vortex-mixed microextraction (SS-BVMME) technique and high performance liquid chromatography (HPLC) analysis. First, ionic liquids 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM]BF4) and n-butanol were mixed to form the supramolecular solvent. After mixing the supramolecular solvent with an aqueous sample to test, a homogenous mixture was formed immediately. BD, HB and NPP were then extracted based on their binding interactions, particularly hydrogen bond formed between their hydroxyl group and the supramolecular solvent. The overall process of sample preparation took only 20min and more than 5 samples could be simultaneously prepared. The minimum detectable concentrations of samples in this method were 0.09925, 0.5429 and 2.428ngmL(-1) for BD, HB and NPP, respectively. Product recoveries ranged from 88% to 103% with relative standard deviations from 0.6% to 4%. For the first time, we report that hydrogen bond plays a key role in SS-BVMME. We also improve the sensitivity significantly to quantify glucocorticoids, which may greatly benefit environmental safety management in China.
    Journal of Chromatography A 08/2013; · 4.61 Impact Factor
  • Qinghai Xia, Yaling Yang, Mousheng Liu
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    ABSTRACT: A simple and sensitive spectrofluorimetric method was developed for the determination of four fluoroquinolone antibacterials namely norfloxacin (NOR), ofloxacin (OFL), ciprofloxacin (CIP) and gatifloxacin (GAT) in honey through charge transfer (CT) complex formation with 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), and then the inclusion complexes of FQs-DDQ with β-cyclodextrin (β-CD) were formed, which resulted in drastic fluorescence enhancement. The effect of several parameters including the concentration of reactants, reaction temperature, time and ultrasonic treatment on the efficiency of the proposed method involving CT reaction and inclusion interaction was systematically investigated. Under the optimum conditions, the limits of detection (LODs) for four FQs in honey varied from 11.6 to 15.4 μg/kg (signal-to-noise ratio (S/N) = 3). The intra- and interday relative standard deviations (RSDs) were 1.6-4.0 % (n = 5) for four FQs. The calibration graph was linear from 42.8 to 1346.8 μg/kg with correlation coefficients not less than 0.9905. The recoveries of four FQs at three different spiked concentrations in honey samples ranged from 80.9 % to 92.8 %. The results indicated that the method was successfully applied for analyzing FQs in honey.
    Journal of Fluorescence 03/2013; · 1.79 Impact Factor
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    ABSTRACT: A cloud-point extraction (CPE) method using Tergitol TMN-6 (TMN-6) non-ionic surfactant was developed for the extraction and preconcentration of propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) in edible oils. The optimum conditions of CPE were 1.5 % (v/v) Tergitol TMN-6, 1 % (w/v) NaCl, ultrasound-assist 15 min at 49 KHz, 20 min equilibrated at 45 °C, and centrifugation for 10 min at 3,000 rpm. The surfactant-rich phase was then analyzed by reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet detection at 280 nm, under gradient separation, using methanol and 1.5 % (v/v) acetic acid. Under the study conditions, four synthetic phenolic antioxidants (SPAs) were successfully separated within 24 min. Limits of detection in the studied edible oils were in the range of 1.6 to 9.0 ng mL−1. The high recoveries of the spiked edible oils were obtained in the range 90–98 %. The CPE method has been shown to be a potentially useful methodology for the preconcentration of the target analytes, with a preconcentration factor of 25. This method was compared with cloud point extraction (using Triton X-114) and liquid–liquid extraction (using methanol).
    Food Analytical Methods 01/2013; 6(1). · 1.97 Impact Factor
  • Qinghai Xia, Yaling Yang, Mousheng Liu
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    ABSTRACT: An aluminium sensitized spectrofluorimetric method coupled with salting-out assisted liquid-liquid ultrasonic extraction for the determination of four widely used fluoroquinolones (FQs) namely norfloxacin (NOR), ofloxacin (OFL), ciprofloxacin (CIP) and gatifloxacin (GAT) in bovine raw milk was described. The analytical procedure involves the fluorescence sensitization of aluminium (Al(3+)) by complexation with FQs, salting-out assisted liquid-liquid ultrasonic extraction (SALLUE), followed by spectrofluorometry. The influence of several parameters on the extraction (the salt species, the amount of salt, pH, temperature and phase volume ratio) was investigated. Under optimized experimental conditions, the detection limits of the method in milk varied from 0.009μg/mL for NOR to 0.016μg/mL for GAT (signal-to-noise ratio (S/N)=3). The relative standard deviations (RSD) values were found to be relatively low (0.54-2.48% for four compounds). The calibration graph was linear from 0.015 to 2.25μg/mL with coefficient of determinations not less than 0.9974. The methodology developed was applied to the determination of FQs in bovine raw milk samples. The main advantage of this method is simple, accurate and green. The method showed promising applications for analyzing polar analytes especially polar drugs in various sample matrices.
    Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 05/2012; 96:358-64. · 1.98 Impact Factor
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    ABSTRACT: Desenvolveu-se um procedimento de microextração por emulsificação assistida por ultrassom e aperfeiçoada por surfactante (UASEME) combinado com cromatografia líquida de alta eficiência com detector de arranjo de diodos para a determinação de 17b-estradiol (βE2), estrona (E1) e dietilestilbestrol (DES) em água. Ultrassom foi aplicado para auxiliar a emulsificação e Triton X-100 foi usado como dispersor e emulsificante. Tetracloreto de carbono foi usado como solvente extrator. Empregando otimização global, o procedimento foi caracterizado por um intervalo linear aceitável de 10 a 1000 ng mL-1 para βE2, E1 e DES (r > 0,997), exatidão e precisão validadas (RSD 0,85-1,28% (n = 5)) e alta sensibilidade com limites de detecção de 0,200, 0,100 e 0,125 ng mL-1 para βE2, E1 e DES, respectivamente. O procedimento foi aplicado para a análise de amostras típicas de água, com fatores de enriquecimento de 85,29, 173,45 e 97,05 para βE2, E1 e DES, respectivamente, e boas recuperações (≥ 89,82%). De maneira geral, o procedimento desenvolvido foi simples e confiável, com potencial de aplicação em larga escala para análises da água.
    Journal of the Brazilian Chemical Society 04/2012; 23(4):694-701. · 1.28 Impact Factor
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    ABSTRACT: A spectrofluorimetric method is presented for the determination of 2 synthetic phenolic antioxidants (SPAs), butylated hydroxyanisole (BHA), and propyl gallate (PG) in foodstuffs. The proposed method is based on the derivatization of SPAs with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in phosphate buffer of pH 9.0 to yield a highly fluorescent brown product. The optimum experimental conditions have been studied carefully. Linear calibration curves were obtained over the concentration range of 0.20 to 40 μg mL⁻¹ for BHA, and 0.80 to 50 μg mL⁻¹ for PG, using NBD-Cl reagent. The detection limits were 18 ng mL⁻¹ for BHA, 55 ng mL⁻¹ for PG. Intra-day and inter-day relative standard deviations at 3 different concentrations were determined. The high recovery values indicate the accuracy of the proposed methods, and low relative standard deviation values indicate good precision. The results presented in this report show that the applied spectrofluorimetric method is acceptable for the determination of the 2 SPAs in the foodstuffs. Other SPAs, tertiary butyl hydroquinone and butylated hydroxytoluene in foodstuffs do not interfere with the proposed method. PRACTICAL APPLICATIONS: In this spectrofluorimetric method, NBD-Cl as a derivation agent is used to detect synthetic phenolic antioxidants. The method specificity has been greatly improved; there was no interference from other commonly used phenolic substances.
    Journal of Food Science 03/2012; 77(4):C401-7. · 1.78 Impact Factor
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    ABSTRACT: A novel and efficient analytical methodology is proposed for extracting and preconcentrating three kinds of estrogens (17β-estradiol (βE2), estrone (E1), and diethylstilbestrol (DES)) in human urine prior to high-performance liquid chromatography (HPLC) analysis. It is based on the induction of micellar organized medium by using a nonionic surfactant (Tergitol TMN-6) to extract the target estrogens. Ultrasound was applied to enhance the extraction efficiency. Parameters affecting the extraction of target analytes including the concentration of surfactant, temperature, extraction time, sample pH, ionic strength, and centrifuging time were investigated. Under the optimum conditions, the linear range of βE2, E1, and DES was from 5.0 to 1000 ng/ml. All correlation coefficients of the calibration curves were higher than 0.997. The relative standard deviations (RSD, n=5) were 2.36-5.27% and the limits of detection (LOD) were 0.1, 0.2, and 0.1 ng/ml for βE2, E1, and DES in human urine, respectively. The results indicated that the method was successfully applied for analyzing βE2, E1, and DES in human urine.
    Analytical Biochemistry 02/2012; 421(2):378-84. · 2.58 Impact Factor
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    ABSTRACT: A new, simple and rapid method based on dispersive liquid-liquid microextraction (DLLME) was developed for extracting and preconcentrating copper (Cu), nickel (Ni), lead (Pb) and cadmium (Cd) in water samples prior to flame atomic absorption spectrometry (FAAS) analysis. 1-(2-thiazolylazo)-naphthol (TAN) was used as chelating reagents, and non-ionic surfactant Triton X-114 and CCl(4) as disperser solvent and extraction solvent, respectively. Some influential factors relevant to DLLME, such as the concentration of TAN, type and volume of disperser and extraction solvent, pH and ultrasound time, were optimized. Under the optimal conditions, the calibration curve was linear in the range of 10-800 μg L(-1) for Cu and Ni, 10-500 μg L(-1) for Pb, and 10-1,000 μg L(-1) for Cd, respectively. The limits of detection for the four metal ions were below 0.5 μg L(-1), with the enhancement factors of 105, 66, 28 and 106 for Cu, Ni, Pb and Cd, respectively. The relative standard deviations (RSD, n = 6) were 2.6-4.1%. The proposed method was applied to determination of Cu, Ni, Pb and Cd in water samples and satisfactory relative recoveries (93.0-101.2%) were achieved.
    Water Science & Technology 01/2012; 67(2):247-53. · 1.10 Impact Factor
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    ABSTRACT: A cloud-point extraction (CPE) method using Triton X-114 (TX-114) nonionic surfactant was developed for the extraction and preconcentration of propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) from edible oils. The optimum conditions of CPE were 2.5% (v/v) TX-114, 0.5% (w/v) NaCl and 40 min equilibration time at 50 °C. The surfactant-rich phase was then analyzed by reversed-phase high-performance liquid chromatography with ultraviolet detection at 280 nm, using a gradient mobile phase consisting of methanol and 1.5% (v/v) acetic acid. Under the studied conditions, 4 synthetic phenolic antioxidants (SPAs) were successfully separated within 24 min. The limits of detection (LOD) were 1.9 ng mL(-1) for PG, 11 ng mL(-1) for TBHQ, 2.3 ng mL(-1) for BHA, and 5.9 ng mL(-1) for BHT. Recoveries of the SPAs spiked into edible oil were in the range 81% to 88%. The CPE method was shown to be potentially useful for the preconcentration of the target analytes, with a preconcentration factor of 14. Moreover, the method is simple, has high sensitivity, consumes much less solvent than traditional methods, and is environment-friendly. Practical Application: The method established in this article uses less organic solvent to extract SPAs from edible oils; it is simple, highly sensitive and results in no pollution to the environment.
    Journal of Food Science 01/2011; 76(1):C98-103. · 1.78 Impact Factor
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    ABSTRACT: In this work, a 4-chloro-7-nitrobenzodioxazole (NBD-Cl) derivatization coupled with the ultrasound-assisted cloud point extraction (UACPE) method prior to high-performance liquid chromatography with fluorescence detection (HPLC-FLD) analysis was developed for the determination of four widely used fluoroquinolones (FQs) including norfloxacin (NOR), ciprofloxacin (CIP), sarafloxacin (SAR), and gatifloxacin (GAT) in eggs. The derivatives of FQs with NBD-Cl were extracted into the Triton X-114 surfactant-rich phase, which was analyzed by reversed-phase HPLC-FLD. Ultrasound was applied to accelerate the phase separation in extraction and enhance the extraction efficiency of target analytes. Variable parameters affecting the derivatization and UACPE procedure were systematically evaluated and optimized. Under the optimum conditions, four FQs were successfully separated within 30 min through an Agilent TC-C18 column. Good recoveries of 86.2–103.5 %, which were calculated using a range of spiked samples at three concentrations, were obtained by UACPE. The calibration graphs were linear over the range of 1.2–73.0 μg kg−1 for four FQs with correlation coefficients (R) no less than 0.9957. The limits of detection were 0.2, 0.5, 0.3, and 0.4 μg kg−1 for NOR, CIP, SAR, and GAT, respectively. The precisions indicated by relative standard deviations ranged from 0.6 to 4.3 % for both intraday and interday analysis. The proposed method proved to be a selective, sensitive, and eco-friendly approach which was successfully applied to analyze FQs in eggs at the local farmers market, and none of the target analytes were detected in these samples.
    Food Analytical Methods · 1.97 Impact Factor